[1]张诗尧,赵元,鲍男,等.大豆球蛋白直接ELISA 检测方法的建立与初步应用[J].大豆科学,2016,35(04):660-665.[doi:10.11861/j.issn.1000-9841.2016.04.0660]
　ZHANG Shi-yao,ZHAO Yuan,BAO Nan,et al.Development of dcELISA Method for Ｒapid Detection Glycinin of Soybean[J].Soybean Science,2016,35(04):660-665.[doi:10.11861/j.issn.1000-9841.2016.04.0660]
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大豆球蛋白直接ELISA 检测方法的建立与初步应用

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年04期 页码: 660-665 栏目: 出版日期: 2016-07-20
Title:
Development of dcELISA Method for Ｒapid Detection Glycinin of Soybean
作者:
张诗尧; 赵元; 鲍男; 娜仁高娃; 刘丹丹
吉林农业大学动物科学科技学院/动物生产及产品质量安全教育部重点实验室/吉林省动物营养与饲料科学重点实验室，吉林长春130118
Author(s):
ZHANG Shi-yao; ZHAO Yuan; BAO Nan; NA-Ｒen-Gao-Wa; LIU Dan-dan
College of Animal Science and Technology，Jilin Agricultural University /Key Laboratory of Animal Production，Product Quality and Security，Ministry ofEducation /Jilin Key Laboratory of Animal Nutrition and Feed Science，Changchun 130118，China
关键词:
大豆球蛋白;  酶联免疫吸收试验;  大豆抗原;  过敏
Keywords:
Glycinin;  ELISA;  Soybean antigen;  Allergy
分类号:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.04.0660
文献标志码:
A
摘要:
以大豆球蛋白特异性多克隆抗体为一抗，辣根过氧化酶标记抗兔IgG( HPＲ) 为酶标二抗，TMB 为底物，建立一种检测大豆球蛋白直接ELISA 方法。应用该方法对在吉林省部分地区收集的25 个样本进行初步检测，并与竞争ELISA 方法进行对比，每个样品进行6 个重复，结果显示，约64%的样本使用直接ELISA 法的蛋白检出显著高于竞争ELISA 法，且标准差的符合率达到72%以上。本试验建立的直接ELISA 方法具有较高的敏感性和特异性，适于食品及饲料行业中过敏蛋白的批量检测。
Abstract:
The present study presents a direct enzyme-linked immunosorbent assay( ELISA) that allows for the detection oftrace amount of glycinin in soybean. In this assay，rabbit anti-glycinin polyclonal antibody( Pab) was used as primary antibodyand goat anti-mouse igG-horseradish peroxidae( HＲP) was used as secondary antibody. Twenty-five samples collected in partsof Jilin province were preliminary tested by this detection method and compared with competitive ELISA method. Ｒesultsshowed amounts of glycinin in about 64% samples detected by the dcELISA were significantly higher than that detected bycompetitive ELISA and the coincidence rate of the standard deviation( SD) was more than 72%. The method showed a goodsensitivity and specificity and was suitable for quick and large quantitative detection of glycinin in food and feed industry.

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备注/Memo

基金项目: 国家自然科学基金青年基金( 31572439 ) ; 吉林省自然科学基金( 20160101348JC ) ; 国家“十二五”科技支撑计划( 2013BAD17B0) ; 教育厅“十二五”科学技术研究资助项目( 2015198) 。第一作者简介: 张诗尧( 1991-) 女，硕士，主要从事动物营养与饲料科学研究。E-mail: 1151352731@ qq. com。通讯作者: 赵元( 1981-) 女，博士，副教授，主要从事饲料抗营养因子研究。E-mail: zhaoyuan4CL52@126. com。

更新日期/Last Update: 2016-08-23