DU Yu-qian,WANG Ming-jiu,SUO Rong-zhen,et al.Comparison of Salt Tolerance of Forage Soybean Varieties(Lines) Hybrided by Cultivated Soybean and Wild Soybean at Germination and Seedling Stages[J].Soybean Science,2022,41(04):405-412.[doi:10.11861/j.issn.1000-9841.2022.04.0405]
栽培大豆与野生大豆杂交饲用大豆品种（系）萌发期和苗期耐盐性比较
- Title:
- Comparison of Salt Tolerance of Forage Soybean Varieties(Lines) Hybrided by Cultivated Soybean and Wild Soybean at Germination and Seedling Stages
- 文献标志码:
- A
- 摘要:
- 为比较不同饲用大豆萌发期和苗期的耐盐性差异，筛选耐盐性较强的饲用大豆种质，本研究以野生大豆与栽培大豆的杂交后代为试验材料，以其父本作为对照材料，探究杂交后代在耐盐性方面与父本的差异。杂交后代编号为品系500、3-2-1棕黑、8-4-1棕、27-2-1和8-3黑绿；品种材料为S001草实兼用杂交野大豆（简称S001）；对照材料为父本野生大豆。分别观测不同浓度NaCl（50，100，150和200 mmol?L-1）处理对7个材料种子萌发和幼苗生长的影响。结果表明：随着NaCl浓度不断升高，不同材料种子的发芽率、发芽势、发芽指数、发芽活力和芽长均呈现下降趋势，各材料之间存在差异；随着盐胁迫强度的加大，苗期饲用大豆的超氧化物酶（SOD）活性、过氧化物歧化酶（POD）活性、可溶性糖（SS）和丙二醛（MDA）含量均升高，不同材料各抗盐生理指标的升降幅度不同。对萌发期与苗期盐胁迫下的所有指标进行主成分分析和隶属函数综合比较，得出7个材料萌发期与苗期耐盐性的排序为S001＞野生大豆＞8-3黑绿＞8-4-1棕＞品系500＞3-2-1棕黑＞27-2-1。在盐胁迫下，S001的耐盐性超过其父本野生大豆，S001可用于进一步研究野生大豆改良栽培大豆耐盐性的分子遗传机制和耐盐栽培大豆新品种选育的优良大豆种质材料。
- Abstract:
- In order to compare the salt tolerance of different forage soybeans at germination stage and seedling stage， and find higher salt tolerance of forage soybean germplasm， the hybrid offspring of wild soybean and cultivated soybean were used as experimental materials， and the male parent was used as control material to explore the difference of salt tolerance between hybrid offspring and male parent. Hybrids numbered Line 500， 3-2-1 brown-black， 8-4-1 brown， 27-2-1 and 8-3 black-green. The variety material was forage soybean S001 (referred to as S001) and the control material was male wild soybean. The effects of different concentrations of NaCl (50，100，150 and 200 mmol?L-1) on seed germination and seedling growth of seven materials were observed. The results showed that with the increase of NaCl concentration， the germination rate， germination potential， germination index， germination vigor and germination length of seeds of different materials showed a downward trend， and there were differences among materials. With the increase of salt stress intensity， superoxide dismutase (SOD) activity， peroxidase (POD) activity， soluble sugar (SS) and malondialdehyde (MDA) content of feed soybean at seedling stage increased， and the fluctuation ranges of salt resistance physiological indexes of different materials were different. The comprehensive comparison of principal component analysis and membership function of all indexes measured under salt stress at germination stage and seedling stage of all materials showed that the order of salt tolerance of seven materials at germination stage and seedling stage was S001>wild soybean>8-3 black green>8-4-1 brown>Line 500>3-2-1 brown black>27-2-1.Under salt stress， the salt tolerance of S001 was higher than that of its parent wild soybean. S001 can be used for further study on the molecular genetic mechanism of salt tolerance of cultivated soybean improved by wild soybean and used as a excellent soybean germplasm material for breeding new varieties of salt-tolerant cultivated soybean.
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备注/Memo
收稿日期：2021-12-23