REN Zi-yi,WEI Zhen,JIANG Ning,et al.Protective Effects of Equol on Depression-like Behavior of Mice Induced by Chronic Sleep Deprivation[J].Soybean Science,2022,41(03):323-329.[doi:10.11861/j.issn.1000-9841.2022.03.0323]
雌马酚对慢性睡眠干扰所致小鼠抑郁样行为的改善作用
- Title:
- Protective Effects of Equol on Depression-like Behavior of Mice Induced by Chronic Sleep Deprivation
- Keywords:
- equol; antidepressant; oxidative stress; inflammatory factor; corticosterone
- 文献标志码:
- A
- 摘要:
- 为探究雌马酚对慢性睡眠干扰所致小鼠抑郁样行为的改善作用，根据体重将60只ICR雄性小鼠随机分为空白对照组、慢性睡眠干扰模型组、雌马酚低剂量组（10 mg?kg-1）、雌马酚中剂量组（20 mg?kg-1）和雌马酚高剂量组（40 mg?kg-1）。睡眠干扰造模15 d后，依次进行空场实验、悬尾实验和强迫游泳实验3种动物行为学实验，对小鼠海马组织中的超氧化物歧化酶、过氧化氢酶、肿瘤坏死因子α、白细胞介素-1β水平和小鼠血清中的皮质酮水平进行测定。结果显示：雌马酚给药组小鼠在悬尾和强迫游泳实验中的不动时间显著减少，海马组织中的SOD、CAT水平提高，海马组织中的IL-1β、TNF-α和血清中的皮质酮含量降低。该结果表明雌马酚可以显著改善慢性睡眠干扰所致的小鼠抑郁样行为，且该改善作用可能与雌马酚可提高小鼠抗氧化能力、下调脑内促炎因子水平、调节下丘脑-垂体-肾上腺轴功能有关。
- Abstract:
- To explore the effect of equol(Eq) on depression-like behavior of mice induced by chronic sleep deprivation(CSD), we randomly subjected sixty ICR male mice to five groups, the control group， the CSD model group， the low-dose equol group， the medium-dose equol group and the high-dose equol group. The chronic sleep deprivation model was established by continuous sleep deprivation for 15 days. The effects of equol on depression-like behavior of CSD model mice were evaluated by open field， tail suspension and forced swimming behavioral tests. The superoxide dismutase(SOD)， catalase(CAT)， the tumor necrosis factor-α(TNF-α) and interleukin-1beta(IL-1β) activity in hippocampus and corticosterone(CORT) in serum of mice were measured. The results of behavioral tests showed that compared with control group， the immobile time of CSD model mice in the tail suspension and forced swimming tests were both significantly increased， the SOD and CAT activities in hippocampus of CSD mice was significantly decreased， the IL-1β and TNF-α content in hippocampus and the CORT in serum of CSD mice were significantly increased. The results indicated that estrol could significantly improve the depressive behavior of mice induced by chronic sleep disturbance， and the improvement may be related to improving antioxidant capacity， down regulating the level of proinflammatory factors in the brain and regulating the function of hypothalamic pituitary adrenal axis.
参考文献/References:
［1］BREITENSTEIN B， SCHEUER S， HOLSBOER F. Are there meaningful biomarkers of treatment response for depression?[J]. Drug Discovery Today，2014，19(5): 539-561.[2]李凌江，马辛.中国抑郁障碍防治指南(第二版)解读:概述[J].中华精神科杂志，2017，50(3): 167-168. (LI L J， MA X. Interpretation of Chinese guidelines for the prevention and treatment of depressive disorder(Second Edition):Overview[J]. Chinese Journal of Psychiatry， 2017，50(3): 167-168.)[3]过伟峰，曹晓岚，盛蕾，等.抑郁症中西医结合诊疗专家共识[J].中国中西医结合杂志，2020，40(2): 141-148. (GUO W F， CAO X L， SHENG L， et al. Expert consensus on diagnosis and treatment of depression with integrated traditional Chinese and Western medicine[J]. Chinese Journal of the Combination of Chinese and Western Medicine， 2020，40(2): 141-148.)[4]CATENA-DELL′OSSO M， BELLANTUONO C， CONSOLI G， et al. Inflammatory and neurodegenerative pathways indepression: A new avenue for antidepressant development?[J]. Current Medicinal Chemistry， 2011， 18(2): 245-255.[5]冯玉，杨志寅，吉峰.脑源性神经营养因子与抑郁症的研究进展[J].中华行为医学与脑科学杂志，2013(1): 84-86. (FENG Y， YANG Z Y， JI F. Research progress of brain-derived neurotrophic factor and depression[J].Chinese Journal of Behavioral Medical and Brain Science，2013(1): 84-86.)[6]卢丞文，刘颖.大豆异黄酮生理功能的研究进展[J].农业与技术，2017，37(17): 4-5. (LU C W， LIU Y. Research progress on physiological function of soybean isoflavones[J]. Agriculture and Technology， 2017， 37(17): 4-5.)[7]姜雪，迟玉杰，许岩，等.大豆异黄酮代谢终产物-雌马酚的研究进展[J].食品工业科技， 2012， 33(19): 401-403，408. (JIANG X， CHI Y J， XU Y， et al. Research progress of equol， the end product of soybean isoflavone metabolism[J]. Science and Technology of Food Industry， 2012， 33(19): 401-403，408.)[8]郑庆虎，周辉，陈祯祥，等.雌马酚的生物学作用及其在医学领域的研究进展[J].中国皮肤性病学杂志， 2018， 32(1): 105-109. (ZHENG Q H， ZHOU H， CHEN Z X， et al. Biological function of equol and its research progress in medical field[J]. The Chinese Journal of Dermatovenereology，2018，32(1): 105-109.)[9]SETCHELL K D R， BROWN N M， LYDEKING-OLSEN E. The clinical importance of the metabolite equol-A clue to the effectiveness of soy and its isoflavones[J]. The Journal of Nutrition， 2002，132(12): 3577-3584.[10]王萌，任国峰.雌马酚健康效应的研究进展[J].卫生研究，2012，41(5): 874-876.(WANG M， REN G F. Research progress on health effects of equol[J].Journal of Hygiene Research，2012，41(5): 874-876.)[11]LU C， GAO R J， ZHANG Y Y， et al. S-equol， a metabolite of dietary soy isoflavones， alleviates lipopolysaccharide-induced depressive-like behavior in mice by inhibiting neuroinflammation and enhancing synaptic plasticity[J]. Food & Function， 2021，13: 5770-5778.[12]LU C， WANG Y， LV J， et al. Ginsenoside Rh2 reverses sleep deprivation-induced cognitive deficit in mice [J]. Behavior Brain Research， 2018:109-115. [13]JIANG N，WANG H X，LI C C，et al. The antidepressant-like effects of the water extract of Panax ginseng and Polygala tenuifolia are mediated via the BDNF-TrkB signaling pathway and neurogenesis in the hippocampus[J]. Journal of Ethnopharmacology，2021： 267.[14]李孟秋. 黄秋葵种子抗抑郁活性及机制研究[D].天津：天津科技大学，2016: 20.(LI M Q.Antidepressant activity and mechanism of okra seeds[D].Tianjin:Tianjin Unversity of Science and Technology，2016: 20.)[15]李鹏，刘瑛丽，骆彤.历代医家茶疗治疗睡眠障碍浅谈[J].中医药临床杂志，2019，31(4): 630-633.(LI P，LIU Y L，LUO T.Discussion on the treatment of sleep disorders by tea therapy of ancient doctors[J].Clinical Journal of Traditional Chinese Medicine，2019，31(4): 630-633.)[16]DANIELA T D，GIUSEPPE C G，LUIGI D L，et al. Long-term impact of earthquakes on sleep quality[J]. PLoS One，2013，8(2):e55936.[17] RIEMANN D，BERGER M，ULRICH V.Sleep and depression-results from psychobiological studies: An overview[J]. Biological Psychology，2001，57(1):67-103. [18]武宏伟.人参皂苷水解产物对慢性应激所致学习记忆功能减退的改善作用[D].北京:北京协和医学院，2015.(WU H W.Effect of ginsenoside hydrolysate on learning and memory impairment induced by chronic stress[D].Beijing: Beijing Union Medical College，2015.)[19]高江晖，卜兰兰，石哲，等.不同时间睡眠干扰所致小鼠的类抑郁样行为[J].中国实验动物学报，2011，19(5): 405-409.(GAO J H，BU L L，SHI Z，et al.Depression like behavior induced by sleep disturbance at different time in mice[J].Acta Laboratorium Animalis Scientia Sinica，2011，19(5): 405-409.)[20]HAMMEN C. Stress and depression[J]. Annual Review of Clinical Psychology， 1(1): 293-319.[21]RAZA M U. The regulation of corticosteroid receptors in response to chronic social defeat[J]. Neurochemistry International， 2017,108:397-409.[22]ZAHORODNA A， TOKARSKI K， BIJAK M. Repeated corticosterone administration increases excitatory effect of 5-HT4 receptor agonist in the rat hippocampus[J]. Polish Journal of Pharmacology， 2000， 52(2): 107-109.[23]RAEDLER T J. Inflammatory mechanisms in major depressive disorder[J]. Current Opinion in Psychiatry，2011，24(6):519-525.[24]张永艳. 抑郁症状与血清氧化应激以及炎症反应的相关性研究[D].石家庄:河北医科大学，2018: 25-26.(ZHANG Y Y.Correlation between depressive symptoms and serum oxidative stress and inflammatory reaction[D].Shijiazhuang:Hebei Medical University，2018: 25-26.)[25]MOHAMMADI A B，TORBATI M，FARAJDOKHT F,et al.Sericin alleviates restraint stress induced depressive and anxiety-like behaviors via modulation of oxidative stress， neuroinflammation and apoptosis in the prefrontal cortex and hippocampus[J]. Brain Research，2019，1715:47-56.[26]MEYSAM A，YONG-KU K，GISELLI S，et al. Evidence for additionally increased apoptosis in the peripheral blood mononucle-ar cells of major depressive patients with a high risk for suicide[J]. American Journal of Medical Genetics. Part B， Neuropsychiatric genetics：The official publication of the International Society of Psychiatric Genetics，2018，177(4):388-396.[27]TAN X N，DU X X，JIANG Y T，et al. Inhibition of autophagy in microglia alters depressive-like behavior via BDNF pathway in postpartum depression[J]. Frontiers in Psychiatry，2018，9:434.[28]ELSABET A G，NIEVES C B，JSSICA N V，et al. Psychological status in depressive patients correlates with metabolic gene expression[J]. CNS Neuroscience & Therapeutics，2017，23(10):843-845.
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备注/Memo
收稿日期：2021-10-08