WAN Hui-na,YU Yue-hua,WANG Yi,et al.Bioinformatics and Expression Analysis of GmNAC131 Gene in Soybean[J].Soybean Science,2021,40(02):232-240.[doi:10.11861/j.issn.1000-9841.2021.02.0232]
侵蚀黑土玉米和大豆根部伴生细菌群落结构分析
- Title:
- Bioinformatics and Expression Analysis of GmNAC131 Gene in Soybean
- Keywords:
- Soybean; GmNAC131 gene; NAC transcription factor; Abiotic stress
- 文献标志码:
- A
- 摘要:
- 为分析东北侵蚀黑土中大豆和玉米根部伴生细菌群落结构多样性，促进土壤侵蚀过程中其根部伴生细菌群落结构响应规律的研究，本研究结合LNA-PCR技术和高通量测序方法，模拟东北黑土侵蚀土壤，分析玉米播种期和抽穗期、大豆开花期根部细菌群落结构多样性和差异。结果表明：土壤表层剥离后大豆根部细菌群落多样性降低，而玉米根部细菌群落多样性变化不显著。变形菌门（Proteobacteria）、放线菌门（Actinobacteria）、拟杆菌门（Bacteroidetes）和厚壁菌门（Firmicutes）为大豆和玉米根部伴生细菌共有的优势菌门。属水平上，Acinetobacter、Rhodanobacter、Steroidobacter和Dyella主要在玉米根部获得，而Bacillus、Arenimonas、Variovorax、Propionibacterium、g__norank_p__和Candidate_division_TM7主要在大豆根部发现。本研究首次发现Candidate division TM7也是植物伴生细菌的成员，其序列主要划分为α、β和γ 3个簇，有97.5%的序列属于α簇。研究证实了植物根部伴生细菌群落结构不仅具有宿主特异性，且易受土壤侵蚀影响。
- Abstract:
- NAC genes have the resistant function to the biological and abiotic stress in many crops. In order to explore the function in soybean, this study cloned a NAC gene GmNAC131 from Williams 82 and analyzed the characteristics of the gene and its coding protein sequence, the protein structure and function, and the expression level of the gene in different tissues by bioinformatics. We analyzed the induced expression of abiotic stress at different time periods by fluorescence quantitative. The cDNA length of GmNAC131 was 1 945 bp, and the open reading frame (ORF) was 1 053 bp. GmNAC131 encoded 350 amino acids, with a molecular weight of 39.49 ku and an isoelectric point of 7.62, contained 3 exons and 2 introns. GmNAC131 contained a highly conserved NAC domain at amino acids from 16 to 166. There was no signal peptide, no transmembrane structure, and the protein composition was hydrophobic. GmNAC131 was located in the nucleus with 8 glycosylation sites and 34 phosphorylation sites. GmNAC131 had a high similarity with NAC proteins of Glycine soja, Vigna unguiculata, Mucuna pruriens, and Cajanus cajan, and shared the same branch with GsNAC. Transcriptome data showed that GmNAC131 gene was expressed in different tissues of soybean, with the highest expression in root and the lowest expression in leaf. The expression of GmNAC131 gene fluctuated during 24 h of low temperature stress at 4 ℃, the expression peaked at 12 h under 250 mmol?L-1 NACL stress, reached its maximum at 0.5 h under 30% PEG6000 stress and reached its maximum at 6 h under 100 mol?L-1 ABA stress.It suggested that the gene might be involved in abiotic stresses such as salt tolerance and drought tolerance in soybean.
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备注/Memo
收稿日期：2020-10-22