ZHAO Xiao-yan,CAO Yue,DONG Rui-meng,et al.Isolation, Identification and Growth Promoting Effect of an Endophytic Bacteria YDX14 in Wild Soybean[J].Soybean Science,2021,40(02):224-231.[doi:10.11861/j.issn.1000-9841.2021.02.0224]
一株野生大豆内生细菌YDX14菌株的分离、鉴定及促生效应研究
- Title:
- Isolation, Identification and Growth Promoting Effect of an Endophytic Bacteria YDX14 in Wild Soybean
- 文献标志码:
- A
- 摘要:
- 为筛选具有促生效应的内生细菌资源，从野生大豆叶片中分离筛选出1株内生细菌菌株YDX14，采用形态学观察和分子生物学分析方法对该菌株进行鉴定；通过测定菌株溶磷能力、产 IAA 能力、产 ACC 脱氨酶能力等研究菌株的促生特性；通过水培促生试验测定菌株对小麦幼苗的促生作用，并采用单因素和正交试验确定菌株的最佳发酵条件。结果表明：YDX14菌落为白色圆形，表面粘稠有光泽，边缘整齐，呈杆状，大小为(2.0～3.2) μm×(0.9～1.2) μm，是一种有芽孢，有荚膜，有鞭毛的革兰氏阴性细菌，为普罗威登斯菌属（Providencia）菌株；菌株具有溶磷能力，溶磷量为 46 mg?L-1；且具有产 IAA 能力，达到19.97 mg?L-1；ACC酶活性达到8.59 μmol?mg-1?h-1；菌株处理小麦10 d后，促生效果最显著，株高、根长、叶干重与根干重分别增加了58.46%、81.89%、54.87%和29.9%；可溶性糖和可溶性蛋白含量均高于对照；其最佳发酵条件是以牛肉膏蛋白胨培养基为基础，葡萄糖1%、酵母膏1%、氯化钙2%、磷酸二氢钾0.1%，pH5.0。
- Abstract:
- Abstract: by morphological observation and molecular biological analysis. The growth-promoting characteristics of the strain were studied by measuring the ability to dissolve phosphorus, producing IAA, and producing ACC deaminase. The growth-promoting effect of strain on wheat seedlings was determined by hydroponic growth-promoting test, and the optimum fermentation conditions of strain were determined by a single factor experiment and orthogonal experiment. The results showed that YDX14 colony was white and round, with a sticky and shiny surface, neat edge and rod shape, and its size was (2.0 - 3.2) μm × (0.9 - 1.2) μm. It was a gram-negative bacterium with spores, capsules, and flagella, belonging to Providencia. The strain had the ability to dissolve phosphorus, and the amount of dissolving phosphorus was 46 mg?L-1. The IAA production capacity reached 19.97 mg?L-1. The ACC enzyme activity reached 8.59 μmol?mg-1?h-1. After 10 days of wheat treatment, the growth-promoting effect was the most significant, and the plant height, root length, leaf and root dry weight increased by 58.46%, 81.89%, 54.87% and 29.9% respectively. The contents of soluble sugar and soluble protein were higher than those of control. The optimum fermentation conditions were based on beef paste peptone medium, glucose 1%, yeast extract 1%, calcium chloride 2%, potassium dihydrogen phosphate 0.1%, and pH5.0.
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备注/Memo
收稿日期：2020-12-18