[1]刘文博,陈丽茹,王睿鹏,等.不同容重大豆品种对人工老化胁迫的反应机制研究[J].大豆科学,2021,40(01):59-67.[doi:DOI：10.11861/j.issn.1000-9841.2021.01.0059]
　LIU Wen-bo,CHEN Li-ru,WANG Rui-peng,et al.Study on Response Mechanism of Soybean Cultivars with Different Bulk Densities to Artificial Aging Stress[J].Soybean Science,2021,40(01):59-67.[doi:DOI：10.11861/j.issn.1000-9841.2021.01.0059]
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不同容重大豆品种对人工老化胁迫的反应机制研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第40卷 期数: 2021年01期 页码: 59-67 栏目: 出版日期: 2021-01-20
Title:
Study on Response Mechanism of Soybean Cultivars with Different Bulk Densities to Artificial Aging Stress
文章编号:
2021,40(1)：059-067
作者:
刘文博; 陈丽茹; 王睿鹏; 张婧; 姚兴东; 谢甫绨; 关欣
（沈阳农业大学 大豆研究所，辽宁 沈阳110866）
Author(s):
LIU Wen-bo;  CHEN Li-ru;  WANG Rui-peng;  ZHANG Jing;  YAO Xing-dong;  XIE Fu-ti;  GUAN Xin
（Soybean Research Institute, Shenyang Agricultural University, Shenyang 110866,China）
关键词:
容重;  大豆种子;  发芽;  人工老化
Keywords:
Bulk density;  Soybean seeds;  Germination;  Artificial aging
DOI:
DOI：10.11861/j.issn.1000-9841.2021.01.0059
文献标志码:
A
摘要:
为探讨经过人工老化处理对不同容重大豆品种种子发芽和种子内抗氧化酶的影响，为大豆种子合理储存提供理论依据。以容重不同的32个大豆品种为试材，经过3种人工老化处理（高温高湿、低温和渗透胁迫）后，测定了这32个大豆品种的种子活力、种子细胞膜透性、脂质过氧化和抗氧化酶活性，从而分析不同容重品种对人工种子老化处理的响应机制。结果表明：未经人工处理时，容重大的品种，发芽指数高、种子活力大。经过人工处理后，容重大的品种，发芽指数下降幅度小，同时种子浸出液的外渗可溶性糖和丙二醛（MDA）含量上升幅度也小。3种人工老化处理会使大豆种子中SOD活性增加、POD活性减弱。容重越大的品种，SOD活性越高，但3种人工老化处理对CAT活性的影响则不同。说明不同容重大豆品种的种子其抗老化和逆境的能力不同，大豆种子的容重对种子活力、萌发机制和生理生化质变有直接的影响。
Abstract:
In order to investigate the effect of artificial aging treatment on seed germination and antioxidant enzymes in soybean varieties with different bulk density, the theoretical basis for rational storage of soybean seeds was provided. With 32 soybean varieties with different bulk densities as test materials, through three kinds of artificial aging treatment (high temperature and high humidity, low temperature and osmotic stress), the seed activity, cell membrane permeability, lipid peroxidation and antioxidant enzyme activity of the 32 soybean varieties were measured and the response mechanism of different bulk density cultivars to artificial seed aging treatment was analyzed.The results show that, without manual treatment, the varieties with large capacity had high germination index and seed vigor, after artificial treatment, the germination index of the varieties with large capacity decreased slightly, and the content of exosmosis soluble sugar and malondialdehyde (MDA) in the seed leach solution also increased slightly.Three kinds of artificial aging treatment increased SOD activity and decreased POD activity in soybean seeds.The activities of SOD were higher in the varieties with larger bulk density, but the effects of three artificial aging treatments on the activities of CAT were different.The ability of anti-aging and anti-adversity of soybean seeds with different bulk density were different, and the bulk density of soybean seed had direct influence on seed vigor, germination mechanism and physiological and biochemical changes.

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备注/Memo

国家重点研发计划（2017YFD0101306-04）。

更新日期/Last Update: 2021-02-09