SHEN Jia-cheng,ZHANG Xiao-li,HUANG Jian-li,et al.Genome Wide Association Analysis on Water-Soluble Protein in Soybean[J].Soybean Science,2020,39(04):509-517.[doi:10.11861/j.issn.1000-9841.2020.04.0509]
大豆水溶性蛋白质的全基因组关联分析
- Title:
- Genome Wide Association Analysis on Water-Soluble Protein in Soybean
- Keywords:
- Soybean ［Glycine max (L.) Merr.］; Water-soluble protein; Genome-wide association analysis; Mining for candidate gene
- 文献标志码:
- A
- 摘要:
- 为进一步解析中国大豆种质水溶性蛋白的遗传基础，为大豆高水溶性蛋白质的分子标记辅助选择育种及品质改良提供理论依据，本研究以224份大豆种质为试验材料，于2017和2018年对大豆水溶性蛋白质含量进行测定，利用1 514个高质量的SNP标记分别对2017、2018年水溶性蛋白质含量及两年均值进行全基因组关联分析，共检测到18个显著关联的SNP标记，这些SNP标记涉及16个位点，有8个位点至少被检测到2次，其余8个位点仅被检测到1次，表明其受环境因素影响较大。16个位点中有7个尚未见报道，分别位于8、11、13、14和15号染色体上，是新发现的控制大豆水溶性蛋白的位点。对表型变异解释率较高且稳定关联的2个位点qWSPC7和qWSPC8-1候选区间内的基因进行预测，共获得25个候选基因，其中有7个基因（Glyma.07g195000、Glyma.08g103100、Glyma.08g108900、Glyma.08g105100、Glyma.08g107800、Glyma.08g107700和Glyma.08G115800）在大豆籽粒、根或根瘤中具有较高的表达水平。这些基因可作为水溶性蛋白质的候选基因，可能具有调控大豆水溶性蛋白质的功能。
- Abstract:
- In order to further analyze the genetic basis of water-soluble protein in Chinese soybean collection, this study investigated the water-soluble protein content phenotype of 224 soybean collection of 2017 and 2018 years. Genome-wide association study (GWAS) of WSPC was performed in 2017, 2018 and the average across of the two years with 1 514 high-quality SNP markers.A total of 18 significantly associated SNPs were detected. These SNPs involved 16 sites, with 8 sites were stably detected in at least two times, and the remaining 8 loci were detected only in a single environment, indicating that it was greatly affected by environmental factors.Seven of the 16 loci have not been reported, located on chromosomes 8, 11, 13, 14, and 15 respectively. They are newly discovered loci that control the water-soluble protein of soybean. Prediction of genes in the two candidate regions of qWSPC7 and qWSPC8-1 with high phenotypic variation interpretation rates and stable associations obtained a total of 25 candidate genes, of which 7 genes (Glyma.07g195000, Glyma.08g103100, Glyma.08g108900, Glyma.08g105100, Glyma.08g107800, Glyma.08g107700 and Glyma.08G115800) had higher expression levels in soybean seed, roots or nodules. These genes can be used as candidate genes for water-soluble proteins and may have the function of regulating soybean water-soluble proteins. This study provides theoretical basis for molecular marker-assisted selection breeding and quality improvement of soybean high water-soluble proteins.
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