HE De-xin,LI Zhi-gang,ZHAO Li-mei,et al.Relationship Between Endogenous Hormones, Gene Expression and Senescence in Soybean Male Sterile Lines[J].Soybean Science,2020,39(02):205-211.[doi:10.11861/j.issn.1000-9841.2020.02.0205]
大豆不育系内源激素及基因表达与衰老的关系
- Title:
- Relationship Between Endogenous Hormones, Gene Expression and Senescence in Soybean Male Sterile Lines
- Keywords:
- Soybean male sterile line; Endogenous hormone; Gene; Green retention
- 文献标志码:
- A
- 摘要:
- 为探究大豆不育系产生持绿现象的原因，选用异交结实率不同的大豆不育系和配套保持系为材料，通过测量鼓粒期大豆叶片中生理生化指标含量以及调控衰老相关基因GmSARK、GmCYN1和GmSGR1的相对表达量，分析植物内源激素和基因表达与衰老之间的关系。结果表明：不育系的赤霉素、细胞分裂素含量高于其同型保持系；脱落酸含量、GmSARK、GmSGR1、GmCYN1基因的相对表达量低于其同型保持系；生长素、乙烯含量与其同型保持系间无显著性差异。大豆不育系与其同型保持系脱落酸、乙烯和细胞分裂素共同调节大豆衰老基因的表达程度，进而影响大豆衰老，是引起大豆不育系持绿现象的部分原因。
- Abstract:
- In order to investigate the causes of green persistence in soybean sterile lines, this study selected soybean sterile lines and supporting maintenance lines with different outcrossing and seed setting rates as materials, measured the content of physiological and biochemical indicators in soybean leaves during the drum kernel stage and regulating aging-related genes GmSARK, and the relative expression of and cytokinin in male sterile lines were higher than those in their homologous maintainers. The relative expressions of abscisic acid content, GmSARK, GMSGR1 and GMCYN1 genes were lower than those in their homologous maintainers. There was no significant difference in the contents of auxin and ethylene between their homologous maintainers. Abscisic acid, ethylene and cytokinin of soybean male sterile lines and their homologous maintainers may regulate the expression of senescence genes in soybean, and then affect the senescence of soybean, which is part of the reason for the green phenomenon of soybean male sterile lines.
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备注/Memo
收稿日期：2019-09-17