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[1]张雅娟,曹永策,李曙光,等.夏大豆重组自交系群体NJRIMN开花期和株高QTL定位[J].大豆科学,2018,37(06):860-865.[doi:1011861/jissn1000-98412018060860]
　ZHANG Ya-juan,CAO Yong-ce,LI Shu-guang,et al.Mapping QTL for Flowering Time and Plant Height in a Summer-sowing Soybean RIL Population NJRIMN [J].Soybean Science,2018,37(06):860-865.[doi:1011861/jissn1000-98412018060860]

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夏大豆重组自交系群体NJRIMN开花期和株高QTL定位

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷期数: 2018年06期页码: 860-865 栏目: 出版日期: 2018-11-20

Title:: Mapping QTL for Flowering Time and Plant Height in a Summer-sowing Soybean RIL Population NJRIMN

作者:: 张雅娟¹; 曹永策¹; 2; 李曙光¹; 常芳国¹; 孔杰杰¹; 盖钧镒¹; 赵团结¹; （1.南京农业大学大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室(综合)/作物遗传与种质创新国家重点实验室/江苏省现代作物生产协同创新中心，江苏南京 210095； 2.延安大学生命科学学院，陕西延安 716000)

Author(s):: ZHANG Ya-juan¹; CAO Yong-ce¹; 2; LI Shu-guang¹; CHANG Fang-guo¹; KONG Jie-jie¹; GAI Jun-yi¹; ZHAO Tuan-jie¹; (1Soybean Research Institute of Nanjing Agricultural University/ National Center for Soybean Improvement/ Key Laboratory for Biology and Genetic Improvement of Soybean (General), Ministry of Agriculture/ National Key Laboratory for Crop Genetic and Germplasm Enhancement/ Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210095, China; 2 College of Life Sciences, Yan’an University, Yan’an 716000, China)

关键词:: 夏大豆; 开花期; 株高; QTL定位; 性状相关

Keywords:: Summer-sowing soybean; Flowering time; Plant height; QTL mapping; Trait correlation

DOI:: 1011861/jissn1000-98412018060860

文献标志码:: A

摘要:: 大豆开花期和株高是存在相关性的重要育种目标性状，但江淮夏大豆该性状研究相对较少。为进一步解析这2个相关性状的遗传基础，本研究以夏大豆重组自交系群体NJRIMN为试验材料，利用QTLNetwok 21软件基于混合线性模型的复合区间作图法（MCIM）和WinQTLCart 25软件的多性状复合区间作图法（MT-CIM），对该供试材料5个环境下开花期和株高性状进行QTL定位。研究结果表明利用MCIM法定位到8个开花期加性QTL，其中qFT-6-2和qFT-11-1存在显著的加性与环境互作效应；还定位到11对开花期上位性QTL，其与环境的互作均不显著。加性效应共解释了7130%的开花期表型变异，而上位性效应只占888%。定位到6个株高加性QTL，其中qPH-6-1、qPH-12-1和qPH-19-2存在显著的加性与环境互作效应；还定位到4对株高上位性QTL，其中qPH-8-1和qPH-16-1存在显著的上位性与环境互作效应。加性效应共解释了4104%的株高表型变异，上位性效应可解释1445%的表型变异。共发现4个同时控制开花期和株高的QTL，其中位于6、10和19号染色体上的位点分别与E1、E2和Dt1基因位置重叠。

Abstract:: Flowering time and plant height of soybean are important target traits for soybean breeding with significant correlation However, relatively less studies of the traits for summer-sowing soybeans in the region between lower Yangtze River and Huai river were conducted Mapping QTLs for these two traits by using recombinant inbred line populations can provide deep knowledge for their genetic basis QTL mapping for flowering time and plant height in NJRIMN was conducted by using WinQTLCart25 with multiple-trait composite interval mapping (MT-CIM) and mixed-model based composite interval mapping (MCIM) via QTLNetwok21 The results showed that 8 additive QTLs for flowering time were detected by MCIM method, among which qFT-6-2 and qFT-11-1 had significant additive and environmental interaction effects Eleven pairs of epistatic QTLs were also mapped, but there was no significant interaction with environment The additive effects totally explained 7130% of the phenotypic variation for flowering time, while epistatic effects accounted for only 888% Six additive QTLs for plant height were mapped, among which qPH-6-1, qPH-12-1 and qPH-19-2 had significant additive and environmental interaction effects Four pairs of epistatic QTLs were also mapped, and there were significant epistasis and environmental interaction effects in the epistasis between qPH-8-1 and qPH-16-1 The additive effects totally explained 4104% of the phenotypic variation for flowering time, while epistatic effects accounted for 1445% Five QTLs simultaneously controlling flowering time and plant height were found, and the three QTLs on chromosome 6, 10 and 19 were overlapped with E1, E2 and Dt1 gene

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备注/Memo

收稿日期：2018-07-01

基金项目：国家重点研发计划课题（2016YFD0100201）；长江学者和创新团队发展计划资助（PCSIRT-17R55）；中央高校基本科研业务费专项资金资助（KYT201801）；江苏省现代作物生产协同创新中心项目（JCIC-MCP）。

第一作者简介：张雅娟（1992-），女，硕士，主要从事大豆分子育种研究。E-mail: 2283519197@qqcom。

通讯作者：赵团结（1969-），男，博士，教授，主要从事大豆遗传育种研究。E-mail: tjzhao@njaueducn。

更新日期/Last Update: 2018-12-04