[1]常 玮,王 娟,于 洋,等.基于F1家系关联群体的野生大豆优异基因挖掘方法研究[J].大豆科学,2018,37(01):50-57.[doi:10.11861/j.issn.1000-9841.2018.01.0050]
　CHANG Wei,WANG Juan,YU Yang,et al.Study of Mining Excellent Genes in Glycine soja by F1 Family Association Population[J].Soybean Science,2018,37(01):50-57.[doi:10.11861/j.issn.1000-9841.2018.01.0050]
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基于F1家系关联群体的野生大豆优异基因挖掘方法研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年01期 页码: 50-57 栏目: 出版日期: 2018-01-20
Title:
Study of Mining Excellent Genes in Glycine soja by F1 Family Association Population
作者:
常 玮; 王 娟; 于 洋; 陈吉宝
（南阳师范学院 农业工程学院，河南 南阳 473061）
Author(s):
CHANG Wei;  WANG Juan;  YU Yang;  CHEN Ji-bao
(College of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China)
关键词:
野生大豆; 优异基因; F1家系关联群体; 关联分析
Keywords:
Glycine soja;  Excellent genes;  F1 family association population;  GWAS
分类号:
565.1
DOI:
10.11861/j.issn.1000-9841.2018.01.0050
文献标志码:
A
摘要:
摘 要：为了研究F1家系关联群体在野生大豆优异基因挖掘中的效果，以大豆HapMap数据为基础，以大豆食心虫抗性为例，分别考虑不同遗传模型、基因数目、群体大小及遗传力大小，模拟自交系群体基因型及表型，并采用顶交的方式模拟生成F1家系关联群体，模拟其表型，在基因型与表型模拟的基础上，采用全基因组关联分析的方法比较二者的检测效力。经比较，F1家系关联群体在4种遗传模型条件下的平均检出数目分别为3.82，3.82，3.80和3.78，均显著高于自交系群体的平均检出数目（2.08，2.09，2.08和1.87）。分别在一定的群体大小、目标基因数目及遗传力大小下，比较自交系群体和F1家系关联群体的检测效力，结果表明，二者均随群体大小增大、基因数目的减少及遗传力的增大而提升，且在相同条件下，F1家系关联群体的检测效力高于自交系群体。通过顶交的方式构建F1家系关联群体，可有效降低不良基因干扰，因此可大幅提高检测效力，从而使F1家系关联群体检测效果优于野生大豆资源群体。
Abstract:
In this study, we evaluated the detection effect of F1 family association population based on soybean HapMap data, and made a case study of soybean pod borer resistance. The value of genotype and phenotype of both inbred line population and F1 family association population were simulated under 4 different conditions, including genetic models(complete dominance and incomplete dominance), the number of genes, population size and heritability. Based on the simulation results, the detection effect of the two populations in GWAS was compared. As a result, the average numbers of detected genes by F1 family association population in 4 different genetic models were 3.82, 3.82, 3.80 and 3.78, respectively, which were significantly higher than those of inbred line population(2.08, 2.09, 2.08 and 1.87). The result under different conditions also showed that the detection effect of both population were increased with the increasing of population size and heritability, reduced with the increasing of genes number, and the detection effect of F1 population is higher than that of inbred population under the same conditions. Constructing the F1 family association population by Top Cross could effectively reduce the interference of unfavorable allele, thereby make the F1 population a better choice for genome-wide association study when mining the excellent alleles in Glycine soja.

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备注/Memo

收稿日期：2017-06-07

基金项目：河南省科技攻关计划（农业领域）（162102110139）。
第一作者简介：常玮(1984-)，男，博士，讲师，主要从事大豆分子育种研究。E-mail：weichang0@126.com。

更新日期/Last Update: 2018-03-13