[1]陈雪丽,李伟群,王英,等.黑土区连作大豆根际土壤氨氧化古菌群落结构特征及其驱动因子分析[J].大豆科学,2016,35(04):620-625.[doi:10.11861/j.issn.1000-9841.2016.04.0620]
　CHEN Xue-li,LI Wei-qun,WANG Ying,et al.Characterization of Ammonia Oxidizing Archaea Community and Its DrivingFactors in the Ｒhizosphere of Continuous Cropping Soybean in Black Soil[J].Soybean Science,2016,35(04):620-625.[doi:10.11861/j.issn.1000-9841.2016.04.0620]
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黑土区连作大豆根际土壤氨氧化古菌群落结构特征及其驱动因子分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年04期 页码: 620-625 栏目: 出版日期: 2016-07-20
Title:
Characterization of Ammonia Oxidizing Archaea Community and Its DrivingFactors in the Ｒhizosphere of Continuous Cropping Soybean in Black Soil
作者:
陈雪丽; 李伟群; 王英; 王玉峰; 王晓军
( 黑龙江省农业科学院土壤肥料与环境资源研究所/黑龙江省土壤环境与植物营养重点实验室/黑龙江省肥料工程技术研究中心，黑龙江哈尔滨150086)
Author(s):
CHEN Xue-li; LI Wei-qun; WANG Ying; WANG Yu-feng; WANG Xiao-jun
( Institute of Soil Fertilizer and Environment Ｒesources，Heilongjiang Academy of Agricultural Sciences /Key Laboratory of Soil Environment and Plant Nutritionof Heilongjiang Province /Fertilizer Engineering Technology Ｒesearch Center of Heilongjiang Province，Harbin 150086，China)
关键词:
关键词: 黑土;  大豆连作;  根际;  群落多样性;  氨氧化古菌
Keywords:
Black soil;  Soybean continuous cropping;  Ｒizosphere;  Diversity of soil microorganism community;  Ammonia oxidizingarchaea
分类号:
S154. 3
DOI:
10.11861/j.issn.1000-9841.2016.04.0620
文献标志码:
A
摘要:
摘要: 连作导致大豆根圈微环境中物质代谢过程发生变化，其中土壤氮素转化相关的一些功能菌群落组成常用来表征土壤氮素转化能力对连作制度的响应。氨氧化是氮素转化过程中的第一步也是限速步骤，通常氨氧化细菌和氨氧化古菌被认为是氨氧化过程的引擎。本文利用黑龙江省农业科学院土壤肥料与环境资源研究所的大豆长期连作试验田为研究平台，采用qPCＲ 和高通量测序等方法，分析连作大豆根际氨氧化古菌的群落丰度和结构组成。结果表明: 连作2 年和连作17 年处理的氨氧化古菌丰度较其它处理低; 连作大豆根际氨氧化古菌种类来自泉古菌门( Crenarchaeota)和奇古菌门两大类( Thaumarchaeota) ，仍有42%为未分类的古菌种类。氨氧化古菌群落结构与环境因子相关关系分析结果表明: 轮作和连作2 年大豆根际土壤氨氧化古菌群落结构组成与土壤总氮含量、总碳含量呈正相关关系而连作4 年以上处理的氨氧化古菌群落结构组成与pH 和碳氮比呈正相关关系。
Abstract:
Continuous cropping resulted in the changing of metabolism process in the rhizosphere of soybean，while the communitycomposition of nitrogen related functional genes were always used to evaluate the response of the nitrogen conversion capabilityof soil to continuous cropping system. Ammonia oxidizing was the first step in the course of nitrogen transformation andalso the limited speed step，so that ammonia oxidizing bacteria( AOB) and ammonia oxidizing archaea were always consideredthe driver of the ni trogen transformation process. In this study，the methods of qPCＲ and Illumina Miseq sequencing were usedto analyze the ammonia oxidizing archaea( AOA) community composition and quantities in rhizosphere of soybean based on thesoybean continuous cropping located observation in black soil. The results showed that the abundance of Archaea amoA gene inSC2 and SC17 were lower than that in other treatments. AOA in the rhizosphere of soybean belongs to two phylums，Crenarchaeotaand Thaumarchaeota，and still have unclassified AOA accounted for 42%. The correlated analysis between AOA compositionand environment factors indicated that the AOA compositions of MS ( maize-soybean rotation) and SC2 were positiverelated to the contents of total nitrogen and total carbon，then that of treatments with more than continuous cropping years of 4showed positive related to pH and C/N ratio.

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备注/Memo

基金项目: 国家自然科学基金( 41301270) ; 黑龙江省人力资源和社会保障厅留学回国人员科技项目择优资助( 2015) ; 哈尔滨市青年科技创新人才专向基金( 2012ＲFQYN035) ; 哈尔滨市应用技术研究与开发项目( 2014ＲFQYJ082) 。第一作者简介: 陈雪丽( 1980-) ，女，博士，助理研究员，主要从事土壤微生物生态研究。E-mail: xuelichen99@163． com。

更新日期/Last Update: 2016-08-23