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[1]高鑫宇,刘丽君,刘博,等.PEG 模拟干旱对大豆抗氧化酶活性及抗氧化能力的影响[J].大豆科学,2016,35(04):616-619,636.[doi:10.11861/j.issn.1000-9841.2016.04.0616]
　GAO Xin-yu,LIU Li-jun,LIU Bo,et al.Effect of Drought Stress Simulated by PEG on Antioxidant Enzyme Activitiesand Antioxidant Capacity in Soybean[J].Soybean Science,2016,35(04):616-619,636.[doi:10.11861/j.issn.1000-9841.2016.04.0616]

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PEG 模拟干旱对大豆抗氧化酶活性及抗氧化能力的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷期数: 2016年04期页码: 616-619,636 栏目: 出版日期: 2016-07-20

Title:: Effect of Drought Stress Simulated by PEG on Antioxidant Enzyme Activitiesand Antioxidant Capacity in Soybean

作者:: 高鑫宇; 刘丽君; 刘博; 马玉玲; 王瑞奇; 董守坤; ( 东北农业大学农学院，黑龙江哈尔滨150030)

Author(s):: GAO Xin-yu; LIU Li-jun; LIU Bo; MA Yu-ling; WANG Ｒui-qi; DONG Shou-kun; ( College of Agriculture，Northeast Agricultural University，Harbin 150030，China)

关键词:: 干旱; 大豆; 抗氧化酶; 抗氧化能力

Keywords:: Drought; Soybean; Antioxidant enzyme; Antioxidant capacity

分类号:: S565. 1

DOI:: 10.11861/j.issn.1000-9841.2016.04.0616

文献标志码:: A

摘要:: 以黑农44 和黑农65 大豆为试验材料，采用20% 浓度的PEG-6000 模拟干旱胁迫，研究干旱胁迫对大豆叶片开花期抗氧化酶活性及抗氧化能力的影响。结果表明: 随处理时间的延长，大豆叶片超氧化物歧化酶( SOD) 、过氧化物酶( POD) 和过氧化氢酶( CAT) 活性、总抗氧化能力( T-AOC) 、超氧阴离子清除能力( SASC) 和羟自由基清除能力( HＲS) 均随着处理时间的延长而呈先升高后降低的趋势; 在处理时期内，对照组与处理组中，抗旱品种黑农44 的各项指标均高于敏感型品种黑农65。相关性分析可知: 黑农44 和黑农65 的CAT 活性与SOD 活性，SASC 与CAT 活性，HＲS 与SOD 活性，HＲS 与SASC 均呈极显著正相关，HＲS 与T-AOC 显著正相关; 耐旱型品种黑农44 的T-AOC 与POD活性，SASC 与POD 活性，HＲS 与CAT 活性均呈极显著正相关，而敏感型品种黑农65 T-AOC 与POD 活性呈现显著正相关，SASC 与POD 活性，HＲS 与CAT 活性正相关均不显著; 黑农44 的SASC 与SOD 活性显著正相关，黑农65 则表现为极显著正相关，耐旱型品种黑农44 抗氧化酶和抗氧化能力之间的相关程度高于敏感型黑农65。试验表明抗旱大豆品种具有较强的抗氧化酶活性和抗氧化能力。

Abstract:: In this experiment，Heinong 44 ( HN44) and Heinong 65 ( HN65) soybean were used as tested materials，20%concentrations of PEG-6000 was used to simulate drought stress，thus to study the effect of drought stress on soybean leavesantioxidant enzyme activities and antioxidant capacity during anthesis. The results showed that with the treatment time prolonging，soybean leaves superoxide dismutase ( SOD) ，peroxidase ( POD) and catalase ( CAT) activity，total antioxidant capacity(T-AOC) ，superoxide anion scavenging capacity( SASC) and hydroxyl free radical scavenging capacity( HＲS) firstly increasedand then decreased with stress time prolong. In the processing period，the indexes of drought resistant varieties HN44were higher than that of the sensitive varieties HN65 in control and treatment group. Correlation analysis showed that the CATand SOD activity，SASC and CAT activity，HＲS and SOD activity，HＲS and SASC presented extremely remarkable positivecorrelation in HN44 and HN65. However，HＲS was significantly positive correlated with T-AOC in HN44 and HN65，in addition，the T-AOC and POD activity，SASC and POD activity，HＲS and CAT activity presented extremely remarkable positivecorrelation in drought resistant varieties HN44，while the sensitive varieties HN65 T-AOC and POD activity had significantpositive correlation，but the SASC and POD activity，HＲS and CAT activity were positively correlated，but there was not significantdifferent. There was significant positive correlation between SASC and SOD activity in HN44，but there was extremelyremarkable positive correlation in HN65. The degree of correlation between antioxidant enzymes and antioxidant capacity inHN44 is higher than that of sensitive HN65. Thus we concluded that drought resistant soybean varieties had strong antioxidantenzyme activities and antioxidant capacity.

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备注/Memo

基金项目: 国家“十二五”科技支撑计划( 2014BAD11B01) ; 黑龙江省科技攻关项目( GA14B101) ; 教育部博士学科点基金( 20122325120013) 。第一作者简介: 高鑫宇( 1991-) ，男，硕士，主要从事大豆栽培生理研究。E-mail: gaoxinyu19910429@163． com。通讯作者: 董守坤( 1978-) ，男，博士，副研究员，硕导，主要从事大豆栽培生理及保护性耕作研究。E-mail: shoukundong@163． com。

更新日期/Last Update: 2016-08-22