[1]贾宝辉,李娜,于希森,等.大豆胚尖转化体系优化和cry1C*基因转化大豆的研究[J].大豆科学,2015,34(05):910-913,917.[doi:10.11861/j.issn.1000-9841.2015.05.0910]
　JIA Bao-hui,LI Na,YU Xi-sen,et al.Optimization of Soybean Embryonic Tip Regeneration System and Transferring cry1C*?Gene into Soybean[J].Soybean Science,2015,34(05):910-913,917.[doi:10.11861/j.issn.1000-9841.2015.05.0910]
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大豆胚尖转化体系优化和cry1C*基因转化大豆的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年05期 页码: 910-913,917 栏目: 出版日期: 2015-10-25
Title:
Optimization of Soybean Embryonic Tip Regeneration System and Transferring cry1C*?Gene into Soybean
作者:
贾宝辉; 李娜; 于希森; 李涵哲; 李洋; 刘玉婷; 孟凡立; 李文滨
东北农业大学 农学院/大豆生物学教育部重点实验室，黑龙江 哈尔滨 150030
Author(s):
JIA Bao-hui;  LI Na; YU Xi-sen;  LI Han-zhe;  LI Yang;  LIU Yu-ting;  MENG Fan-li;  LI Wen-bin
Agronomy College of Northeast Agricultural University/Key Laboratory of Soybean Biology, Ministry of Education, Harbin 150030, China
关键词:
大豆(Glycine max L); 胚尖; 遗传转化; cry1C
Keywords:
Soybean(Glycine max L);  Embryonic tip;  Genetic transformation;  cry1C*
DOI:
10.11861/j.issn.1000-9841.2015.05.0910
文献标志码:
A
摘要:
以抗大豆食心虫品系东农8004的胚尖为外植体，通过对芽诱导和芽伸长培养基进行优化，建立8004的胚尖转化体系，利用该体系进行cry1C*基因的转化，对获得的抗性再生植株进行分子鉴定和抗虫性鉴定。结果表明：在芽诱导培养基中附加1.67 mg.L-16-BA，丛生芽诱导率最高，达到80%，在芽伸长培养基中附加0.5 mg.L-1 GA3和0.1 mg.L-1.IAA，芽伸长率最高为54%。共获得94株PPT(100 μg.mL-1 )抗性再生植株，PCR检测阳性14株，阳性率为14.89%。对4株T0代阳性植株进行大豆食心虫抗性鉴定，其中C-1植株的食心虫抗性明显高于野生型8004，说明在大豆中表达cry1C*基因能提高大豆对食心虫的抗性。
Abstract:
Abstract: Through the optimization of shoot induction medium and shoot elongation medium, established a embryonic tips transformation system with embryonic tips of Dongnong 8004 and transformed cry1C* gene into soybean by this system. The resistant plants were identified by molecular and insect resistance methods. The results showed that the percentage of shoots induced reached 80% in shoot induction medium with 1.67 mg.L-16-BA and the rate of shoot elongation reached 54% in shoot elongation medium with 0.5 mg?L-1?GA3?and 0.1 mg,L-1?IAA. A total of 94 resistant plants with 100 μg.mL-1PPT were determined by PCR, which included 14 positive lines of transformation and the rate was 14.89%.We found that the cry1C* gene expressed in soybean which could improve the resistance in pod borer. After the resistant identification of soybean pod borer with T0?positive plants, we discovered that the resistance of C-1 was higher than Dongnong 8004.

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备注/Memo

基金项目：国家自然科学青年基金（31201229）；中国博士后科学基金（2012M510914）；黑龙江省博士后基金（LBH-Z11223）；黑龙江省普通高等学校青年学术骨干支持计划项目（1253G008）；东北农业大学博士启动基金（2012RCB07）；东北农业大学2014年大学生SIPT计划创新训练项目。

第一作者简介：贾宝辉（1986-），女，硕士，主要从事大豆基因工程及抗虫方面的研究。E-mail:505400196@qq.com。
通讯作者：李文滨（1958-）, 男，教授，博导，主要从事大豆遗传育种研究。E-mail: wenbinli@yahoo.com。

更新日期/Last Update: 2015-11-09