PDF下载 +分享

微信公众号：大豆科学

[1]李建东,景小原,燕雪飞.黄淮野生大豆表型性状多样性研究[J].大豆科学,2015,34(05):741-751.[doi:10.11861/j.issn.1000-9841.2015.05.0741]
　LI Jian-dong,JING Xiao-yuan,YAN Xue-fei.Study on Phenotypic Diversity of Wild Soybean in Huang-Huai Populations[J].Soybean Science,2015,34(05):741-751.[doi:10.11861/j.issn.1000-9841.2015.05.0741]

点击复制

黄淮野生大豆表型性状多样性研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷期数: 2015年05期页码: 741-751 栏目: 出版日期: 2015-10-25

Title:: Study on Phenotypic Diversity of Wild Soybean in Huang-Huai Populations

作者:: 李建东; 景小原; 燕雪飞; 沈阳农业大学农学院，辽宁沈阳 100866

Author(s):: LI Jian-dong; JING Xiao-yuan; YAN Xue-fei; College of Agronmy, Shenyang Agricultural University, Shenyang 110866, China

关键词:: 野生大豆; 农艺性状; 种群变异; 表型多样性; 巢式设计; 聚类分析; 相关分析

Keywords:: Wild soybean; Agronomic traits; Population variation; Phenotypic diversity; Nest design; Hierarchical cluster analysis; Correlation analysis.

DOI:: 10.11861/j.issn.1000-9841.2015.05.0741

文献标志码:: A

摘要:: 以黄淮地区具有代表性的4个野生大豆居群为研究试材，对百粒重、生育期、荚果长、荚果宽、株高、每荚粒数、分枝数、茸毛色、主茎、粒色、萌发时间、叶形12个重要农艺性状进行系统比较分析，运用变异系数、多重比较和巢式方差分析研究了居群间和居群内的表型变异；应用相关分析揭示了表型性状间及其与地理、气候因子间的相关性以及表型变异的地理格局；并且运用主成分分析、非加权配对算数平均分（UPGMA）聚类分析进行了居群分类。结果表明：居群间的变异远大于居群内的变异，居群分化较大，百粒重与纬度显著正相关、生育期与纬度显著负相关，粒色与经度、年降水量显著相关，其它9个性状没有特别明显的相关关系；表型性状的欧式距离与地理距离相关不显著。百粒重、荚果长、荚果宽、茸毛色、叶形5个表型性状指标是造成表型差异的主要形态性状。分枝数与百粒重呈显著负相关，荚果宽与每荚粒数、萌发时间呈显著相关水平。利用群体间欧式距离表明，在欧式距离4.67处可以将居群分为3类，在欧式距离5.12处可以将居群分为2类。

Abstract:: A set of 114 Glycine.soja covering 4 populstions of Huang-Huai were selected to study the phenotype variability and relationships.Twelve morphological characteristics such as 100-seed weight, maturity, pod length, pod width, plant height, seeds number per pod, branch number, pubescence color, main stem, seed coat color, germination time, leaf shape were discussed by comparing and analyzing with the methods of nest design, multi-comparsion, related analysis and hierarchical cluster analysis .The results indicated that there were significant differences in phenotypic variation among/within populations. In different individuals,simpson index ranged from 0.220 5 to 0.951 2, Shannon-Weaver index ranged from 0.067 7 to 3.591 6, and the F value varying from 0.53 to 18.68.In different individuals,the variation coefficient of seed colour was 68.93%,while the variation coefficient of maturity was only 5.52%. Most phenotypic traits were significiantly correlated. 100-seed weight and maturity were significantly positive correlated with latitude, the correlation coefficient were respectively 0.984* and 0.929*.Seed color was significantly correlated with longitude and annual precipitation (0.981* and 0.968*). It also showed that there was no significant correlation of Glycine-soja between phenotypic characteristics and geographic distance.100-seed weight, pod length, pod width, pubescence color and leaf shape were the main morphological characters of phenotypic differences. The Huang-Huai populations could be divided into three groups according to the UPGMA cluster analysis when the educlidean distance was 4.67 and two groups when the euclidean distance was 5.12.Phenitypic variation within populations was greatly smaller than that between populations.Compared to other characters,the stability of seed was higher than that of other traits.

参考文献/References:

［1］顾万春,王琪,游应天,等.森林遗传资源学概论［J］.北京:科学出版社,1998:16-20(Gu W C,Wang Q,You Y T,et al.The study of forest genetic resources［J］.Beijing:Science Press,1998:16-20)

［2］李斌,顾万春,卢宝明.白皮松天然群体种实表型多样性研究［J］.生物多样性,2002,10(2):181-188(Li B, Gu W C, Lu B M. A study on phenotypic diversity of seeds and cons characteristics in Pinus bungeana［J］.Biodiversity Science,2002,10(2):181-188)

［3］李斌,顾万春.白皮松保育遗传学—天然群体遗传多样性评价与保护策略［J］林业科学,2005(1):57-64(Li B,Gu W C.Consevation genetics of Pinus bungeana-Evaluation and conservation of natural populations genetic diversity［J］.Scientia Silvae Sinicae,2005(1):57-64)

［4］Schaal B A, Leverich W J, Rogstad S H. Comparison of methods for assessing genetic variation in plant conservation biology［M］//Falk D A,Holsinger K E. Genetics and conservation of rare plants New York: Oxford University Press,1991:123-134

［5］葛颂.酶电泳资料和系统与进化植物学研究综述［J］武汉植物学研究,1994,12（1）:71-84(Ge S. Reviewed on enzyme electrophoresis data and systematic and evolutionary botany［J］ Wuhan Botanical Research,1994,12（1）：71-84)?

［6］葛颂,洪德元.遗传多样性及其检测方法［M］//钱迎倩,马克平.生物多样性研究的原理与方法.北京:中国科学技术出版社,1994:123-140(Ge S, Hong D Y. The genetic diversity and its detection methods［M］//Qian Y Q, Ma K P. The principle and methods of biodiversity research. Beijing: Science and Technology of China Press, 1994,123-140)

［7］邱丽娟,常汝镇.大豆种质资源描述规范和数据标准［M］.北京:中国农业出版社,2006(Qiu L J,Chang R Z.The description specification and standard data of G.soja germplasm［M］. Beijing: Agricultural Press, 2006.

［8］中国科学院生物多样性委员会.生物多样性研究的原理与方法［M］.北京:中国科学技术出版社,1994:21-30 (Biodiversity Committee of Sciences in the Chinese Academy. The principle and methods of biodiversity research［M］. Beijing: Science and Technology of China Press, 1994:21-30)

［9］刘德金,肖承和.农业试验设计与分析［M］.北京:中国农业科学技术出版社,2005(Liu D J,Xiao C H.The design and analysis of agricultural experiment［M］.Beijing: Agricultural Science and Technology of China Press, 2005)

［10］何显静等.SAS系统在川百合形态变异式样研究中的运用［J］. 云南大学学报, 2003, 25 (S): 75-77. (He J X,et al. SAS system used in the study of lily variation patterns［J］.Journal of Yunnan University,2003, 25 (S): 75-77)

［11］顾万春.统计遗传学［M］.北京:科学出版社,2004:13-18(Gu W C. Statistical genetics［M］. Science press,2004:13-18)

［12］罗建勋,顾万春.云杉天然群体表型多样性研究［J］.林业科学,2005,41(2):66-71.(Luo J X, Gu W C. Study on phenotypic diversity of natural populations in Picea asperata［J］. Scientia Silvae Sinicae, 2005,41(2):66-71)

［13］Hotelling H.Analysis of a complex of statistical variables into principal components［J］.Journal Educational Psychology, 1933,24:417-441

［14］Wright S.Isolation by distance under diverse systems of mating［J］.Genetics, 1946,31:39-59

［15］徐豹,徐航,庄炳昌,等.中国野生大豆(G.soja)籽粒性状想遗传多样性及其地理分布［J］.作物学报,1995,21(6):733-739.(Xu B, Xu H, Zhuang B C, et al.Study on genetic diversity and their geographical distribution of G.soja in China［J］.Acta Agronomica Sinica, 1995,21(6):733-739)

［16］张林,罗天祥.植物叶寿命及其相关叶性状的生态研究进展［J］.植物生报, 2004,28(6):844-852.(Zhang L,Luo T X. Plant leaf life span and its related ecological research progress of leaf traits［J］. Journal of Plant, 2004,28(6):844-852)

［17］王英姿,洪伟,吴承祯,等.灵石山米储林优势种群不同叶龄叶属性的研究［J］.福建林学院学报, 2009,9(3):284-291(Wang Y Z,Hong W,Wu C Z,et al.Study on the dominant population attributes of different leaf age leaves in Lingshi Sammy［J］. Journal of Fujian Forestry College, 2009,9(3):284-291)

［18］Fuchs E J,Hamrick J L.Genetic diversity in he endangered topical.Gttaizcum sanctm(Zygophyllaceae)［J］Journal of Heredity, 2010,101(3):284-291.

［19］兰彦平,周连第,姚研武,等.中国板栗种质资源的AFLP分析［J］.园艺学报,2010,37(9):1499-1506.(Lan Y P,Zhou L D,Yao Y W,et al.Analysis on the AFLP of Chinese chestnut germplasm resources［J］. Journal of Horticulture, 2010,37(9):1499-1506)

［20］杨静.植物遗传变异及其检测方法［J］. 武汉植物学研究,1991,9:185-195.(Yang J.Infraspecific variation in plant and the exploring methods［J］.Journal of Wuhan Botanical Research, 1991,9:185-195)

［21］葛颂,王慧箐,张春明.巴米山遗传多样性及群体遗传多样性研究［J］.园艺学报, 1997,39:266-271.(Ge S,Wang H Q,Zhang C M.Genetic diversity and population differentitain of Cathaya argyrophylla in Bamian Mounain［J］. Journal of Horticulture,1997,39:266-271)

［22］葛颂,王明霞,陈云文.马尾松同工酶技术及群体结构分析［J］.林业科学, 1988,24:399-409(Ge S,Wang M X,Chen Y W.An analysis if populatio genetic stucture of masson pine by isozyme technique［J］.Scientia Silvae Sinicae, 1988,24:399-409)

［23］Daniel L H, Andrew G C.Principles of population genetics［M］.USA:Sinauer Associates,1989

［24］Hamrick J L,Godt M J W.Allozyeme diversity in plant species［J］. Sinauer, 1990,15:23-31

［25］Volis S, Yakubov B, Irina S, et al.Tests for adaptive RAPD variation in population genetic structure of wild barley, Hordeum spontaneum Koch［J］.Biological Journal of the Linnean Society,2001,74:289-303

［26］Knapp E E,Rice K I Genetic stucture and gene flow in Elymus glaucus(blue wildrye):Implications for native grassland restoration［J］.Restoration Ecology 1996,4:1-10.

相似文献/References:

[1]林凡敏,柏锡,樊超,等.转GsGST14耐盐碱基因大豆的农艺性状调查[J].大豆科学,2013,32(01):56.[doi:10.3969/j.issn.1000-9841.2013.01.013]
　LIN Fan-min,BAI Xi,FAN Chao,et al.Investigation and Analysis of the Main Agronomic Traits of Different Transgenic Soybean Lines with GsGST14 Gene[J].Soybean Science,2013,32(05):56.[doi:10.3969/j.issn.1000-9841.2013.01.013]
[2]张惠君,路茸,王海英,等.始花期追施尿素对早熟菜用大豆农艺性状和产量的影响[J].大豆科学,2013,32(01):68.[doi:10.3969/j.issn.1000-9841.2013.01.016]
　ZHANG Hui-jun,LU Rong,WANG Hai-ying,et al.Effect of Topdressing Urea at R1 on Agronomic Traits and Yield of Early-Mature Vegetable-Type Soybeans[J].Soybean Science,2013,32(05):68.[doi:10.3969/j.issn.1000-9841.2013.01.016]
[3]高越,刘辉,陶波.抗草甘膦野生大豆筛选及其抗性生理机制研究[J].大豆科学,2013,32(01):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
　GAO Yue,LIU Hui,TAO Bo.Screening and Physiological Mechanisms of Resistance to Glyphosate in Wild Soybeans(Glycine soja)[J].Soybean Science,2013,32(05):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
[4]王军卫,侯立江,李? 登,等.野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
　WANG Jun-wei,HOU Li-jiang,LI Deng,et al.Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(05):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
[5]王军卫,侯立江,李登,等. 野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.
　WANG Jun-wei,HOU Li-jiang,LI Deng,et al. Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(05):596.
[6]雍太文,刘小明,肖秀喜,等.不同种子处理对苗期干旱胁迫条件下大豆农艺性状、产量及品质的影响[J].大豆科学,2013,32(05):620.[doi:10.11861/j.issn.1000-9841.2013.05.0620]
　YONG Tai-wen,LIU Xiao-ming,XIAO Xiu-xi,et al.Effects of Different Seed Treatments on Agronomic Properties,Yield and Quality of Soybean under Drought Stress at Seedling Stage[J].Soybean Science,2013,32(05):620.[doi:10.11861/j.issn.1000-9841.2013.05.0620]
[7]王丽燕.硅对野生大豆幼苗耐盐性的影响及其机制研究[J].大豆科学,2013,32(05):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
　WANG Li-yan.Effects of Silicon on Salt Tolerance of Glycine soja Seedlings and Its Mechanism[J].Soybean Science,2013,32(05):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
[8]陈丽丽,王明玖,何丽君,等.野生大豆ISSR体系的优化及其在远缘杂交后代鉴定中的利用[J].大豆科学,2013,32(04):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
　CHEN Li-li,WANG Ming-jiu,HE Li-jun,et al.Optimization for ISSR Reaction System of Wild Soybean and Its Utilization in Distant Hybrid Identification[J].Soybean Science,2013,32(05):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
[9]赵婧,邱强,张鸣浩,等.高产大豆品种的生理特征和产量性状研究[J].大豆科学,2013,32(04):482.[doi:10.11861/j.issn.1000-9841.2013.04.0482]
　ZHAO Jing,QIU Qiang,ZHANG Ming-hao,et al.Physiological Characters and Yield Components of High-yield Soybean Cultivars[J].Soybean Science,2013,32(05):482.[doi:10.11861/j.issn.1000-9841.2013.04.0482]
[10]郑世英,萧蓓蕾,金桂芳.NaCl胁迫对野生大豆和栽培大豆叶绿素及光合特性的影响[J].大豆科学,2013,32(04):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
　ZHENG Shi-ying,XIAO Bei-lei,JIN Gui-fang.Effect of NaCl Stress on Chlorophyll Content and Photosynthetic Characteristics of Glycine soja and Glycine max[J].Soybean Science,2013,32(05):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
[11]李炜,肖佳雷,毕影东,等.黑龙江省野生大豆资源农艺性状和品质性状的遗传多样性分析[J].大豆科学,2015,34(01):9.[doi:10.11861/j.issn.1000-9841.2015.01.0009]
　LI Wei,XIAO Jia-lei,BI Ying-dong,et al.Diversity of Wild Soybean Resources Based on Agronomic and Quality Traits in Heilongjiang Province[J].Soybean Science,2015,34(05):9.[doi:10.11861/j.issn.1000-9841.2015.01.0009]
[12]王岚,孙君明,王连铮.野生大豆资源在海南和北京种植的生育期与部分农艺性状调查简报[J].大豆科学,2018,37(王连铮先生专辑):86.[doi:10.11861/j.issn.1000-9841.2016.06.1047]
　WANG Lan,SUN Jun-ming,WANG Lian-zheng.Brief Report of Growth Period and Agronomic Trait of Wild Soybean Planting in Hainan and Beijing[J].Soybean Science,2018,37(05):86.[doi:10.11861/j.issn.1000-9841.2016.06.1047]

备注/Memo

第一作者简介：李建东（1964-），男，教授，博导，主要从事农业生态系统管理、农田生态学、入侵生态学及生物多样性的研究。E-mail:sylijiandong@126.com。

更新日期/Last Update: 2015-11-07