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[1]汪美汐,胡佳文,马丽,等.超声波法提取异黄酮的研究进展[J].大豆科学,2015,34(04):717-722.[doi:10.11861/j.issn.1000-9841.2015.04.0717]
　WANG Mei-xi,HU Jia-wen,MA Li,et al.Research Progress on Isoflavon Extraction by Ultrasonic Method[J].Soybean Science,2015,34(04):717-722.[doi:10.11861/j.issn.1000-9841.2015.04.0717]

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超声波法提取异黄酮的研究进展

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷期数: 2015年04期页码: 717-722 栏目: 出版日期: 2015-08-25

Title:: Research Progress on Isoflavon Extraction by Ultrasonic Method

作者:: 汪美汐; 胡佳文; 马丽; 刘畅; 万端极; 周宝晗; 湖北工业大学轻工学部，湖北武汉 430068

Author(s):: WANG Mei-xi; HU Jia-wen; MA Li; LIU Chang; WAN Duan-ji; ZHOU Bao-han; Light Industry Division, Hubei University of Technology, Wuhan 430068, China

关键词:: 超声波; 异黄酮; 提取; 研究进展

Keywords:: Ultrasonic; Isoflavones; Extraction; Research progress

DOI:: 10.11861/j.issn.1000-9841.2015.04.0717

文献标志码:: A

摘要:: 通过超声波与传统的加热回流和索氏提取方法、新型的亚临界水萃取方法、正交试验方法和其它辅助的超声提取法的比较，对超声波法提取异黄酮的技术进行总结和归纳。结果表明：超声波能快速、高效的从各种原料中提取异黄酮，并对设备要求较低，因此超声波作为一种经济型的提取方式，是一个可持续发展行业的需求。文章为进一步简化工艺流程, 提高提取率提供了理论基础。

Abstract:: The research progress about isoflavones extraction and purification was briefly reviewed. Compared ultrasonic method with conventional heating reflux extraction and Soxhlet extraction, subcritical water extraction, the orthogonal experiment and other auxiliary ultrasonic extraction method, ultrasonic extraction technology of isoflavones was summarized and analyzed. The results showed that isoflavones could be quickly, high efficiently extracted from various raw materials, and low requirement on equipment by ultrasonic method. So the ultrasonic extraction was an economical alternative to traditional extraction processes, which could meet the industry demand for a sustainable development.This review provided theoretical foundation for further simplifying technological process and improving extraction rate of soybean isoflavones.

参考文献/References:

［1］高秀芝，刘慧，丁雪莲，等.大豆异黄酮的研究与应用进展［J］.食品科学，2004,25(11)：386-391(Gao X Z,Liu H,Ding X L,et al.Progress on the study and application of soybean isoflavones［J］Food Science,2004,25(11):386-391)

［2］孙玲, 魏振承, 徐志宏, 等.大豆异黄酮提取纯化及其抗衰老作用初探［J］.食品科学, 2002,23(8):267-270(Sun L,Wei Z C,Xu H Z,et al.Preliminary study on extraction, purification and anti-senility function of isoflavones［J］.Food Science, 2002,23(8):267-270)

［3］孙克杰，汤坚.异黄酮类化合物在不同氧化体系中的作用研究［J］.食品科学, 2001,22(3):21-26(Sun K J,Tang J.Study on the effect of isoflavones in different oxidation system［J］.Food Science, 2001,22(3):21-26)

［4］刘志胜，李里特，辰巳英三.大豆异黄酮及其生理功能研究进展［J］.食品工业科技，2000，21(1):78-80(Liu Z S,Li L T, Tatsumi Eizo.The research on soybean isoflavones and its physiological functions［J］. Science and Technology of Food Industry,2000, 21(1):78-80)

［5］Terigar B,Balasubramanian S,Boldor D,et al.Continuous microwave-assisted isoflavone extraction system:Design and performance evaluation ［J］.Bioresource Technology,2010,101(7):2466-2471

［6］江英, 邓辉, 党亚丽,等．大豆异黄酮提取条件的研究［J］．食品研究与开发,2004，25(3)：67(Jiang Y,Deng H,Dang Y L,et al.Research of soybean isoflavone extraction conditions［J］Food Research and Development,2004, 25(3):67)

［7］袁龙, 王晓磊，刘建周．大豆中异黄酮提取条件及其在提取液中的存在形式［J］．徐州师范大学学报(自然科学版)，2004, 22(3):55(Yuan L,Wang X L,Liu J Z. Study on the extraction conditions of isoflavones from soybean meal and their existing forms in the extracting solvent［J］. Journal of Xuzhou Normal University (Natural Science Edition),2004, 22(3):55)

［8］王丽娟，张永忠，张丽丽.超声波辅助法提取大豆酱渣饼中大豆异黄酮［J］.中国油脂，2009,34（5）：52-55(Wang L J,Zhang Y Z,Zhang L L.Ultrasound-assisted extraction isoflavone from soybean sauce cake［J］China oils and fats,2009,34(5)：52-55)

［9］张炳文，宋永生，郝征红，等．大豆异黄酮酸水解工艺的研究探讨［J］．中同粮油学报，2003，18(3)：44.(Zhang B W,Song Y S,Hao Z H,et al. Research process on soybean isoflavones acid hydrolysis［J］.Journal of the Chinese Cereals and Oils Association,2003，18(3)：44)

［10］汪海波，刘大川，李永明,等．酸水解法提取大豆异黄酮甙元工艺研究［J］．食品科学，2003，42(4)：98(Wang H B,Liu D C,Li Y M,et al.Acid hydrolysis method to extract soybean isoflavone aglycone［J］.Food Science，2003，42(4)：98)

［11］刘中华，赵锦慧，梁少君.微波辅助提取豆粕中大豆异黄酮［J］大豆科学，2012, 31（6）：993-995 (Liu Z H,Zhao J H,Liang S J.Microwave-assisted extraction of soybean isoflavone from soybean meal［J］Soybean Science,2012,31(6)：993-995)

［12］Zuo Y B,Zeng A W,Yuan X G, et al. Extraction of soybean isoflavones from soybean meal with aqueous methanol modified supercritical carbon dioxide［J］.Journal of Food Engineering,2008,89(4):384-389

［13］陈赟,李建明,章丽娟,等.亚临界水萃取技术及其在天然产物分析中的应用［J］中药材,2009, 32(4):636-641(Chen Y, Li J M, Zhang L J, et al.Subcritical water extraction technology and its application in the analysis of natural products［J］.Journal of Chinese Medicinal Materials,2009,32(4):636-641)

［14］Kamaljit V, Raymond M, Lloyd S, et al.Applications and opportunities for ultrasound assisted extraction in the food industry .A review［J］.Innovative Food Science and Emerging Technologies,2008,9:161-169

［15］刘祥义. 超声波提取元宝枫叶总黄酮方法研究［J］.云南化工，2003，30(1)：27 (Liu X Y.Study on the extraction of flavonoids from the leaves of acer truncatum by ultreasonic treatment［J］.Yunnan Chemical Technology，2003，30（1）：27)

［16］钱丽丽,左锋，唐彦军.大豆异黄酮提取方法的研究进展［J］.黑龙江八一农垦大学学报,2006,18(5):64-67(Qian L L,Zuo F,Tang Y J. Research on several extraction methods of soy isoflavone［J］Journal of Heilongjiang August First Land Reclamation University,2006,18(5):64-67)

［17］史宣明, 岳琳, 武丽荣.大豆异黄酮的提取与精制［J］.中国油脂, 2001, 26(2):3-5(Shi X M, Yue L, Wu L R. The extraction and refining of Soybean isoflavone［J］. China Oils and Fats, 2001, 26(2):3-5)

［18］彭游，余盛禄.大豆异黄酮提取研究最新进展［J］.大豆科学, 2012, 31(2)：320-323.(Peng Y, Yu S L. Advances in extraction of soy isoflavones［J］. Soybean Science, 2012, 31(2):320-323)

［19］Mauricio A, Rostagno, Miguel P, et al.Ultrasoung-assisted extraction of soy isoflavones［J］.Journal of Chromatography A, 2003, 1012(2):119-128

［20］Mauricio A,Rostagno,Miguel P,et al.Ultrasound-assisted extraction of isoflavones from soy beverages blended with fruit juices［J］.Analytica Chimica Acta, 2007, 597: 265-272

［21］谢明杰，宋明，邹翠霞.超声波提取大豆异黄酮［J］.大豆科学，2004，23（1）：75-76.(Xie M J, Song M, Zou C X. Ultrasonic extraction of soybean isoflavones ［J］.Soybean Science,2004, 23(1):75-76)

［22］田琳,尉震,石军, 等.超声波法在大豆异黄酮提取中的应用［J］.科技创新导报，2009(12):111.(Tian L,Wei Z,Shi J,et al.The ultrasonic method in the application of the soybean isoflavone extraction［J］.Science and Technology Innovation Herald,2009(12):111)

［23］肖虹,张晓丽,申纯渥,等.超声乙醇法提取大豆异黄酮的研究［J］.食品与发酵工业，2006(5):138-139 (Xiao H, Zhang X L, Shen C W, et al. Study of extraction isoflavone from soybean by ultrasonic ethanol method［J］Food and Fermentation Industries,2006(5):138-139)

［24］丛艳波.槐角异黄酮的提取、纯化及水解工艺的研究［D］.哈尔滨:东北农业大学,2011.(Cong Y B.Fructus sophorae isoflavone extraction, purification and research of hydrolysis process［D］.Harbin:Northeast Agricultural University,2011)

［25］周丽.葛根异黄酮的提取、纯化及水解工艺的研究［D］.哈尔滨:东北农业大学,2012(Zhou L.The extraction, purification of pueraria isoflavones and hydrolysis process research［D］.Harbin:Northeast Agricultural University,2012)

［26］潘廖明，姚开，贾冬英,等.超声辅助提取大豆异黄酮的研究［J］.中国油脂， 2003, 28(11):85-87(Pan L M,Yao K,Jia D Y,et al. Extraction of soybean isoflavone assisted by ultrasonic wave［J］.China Oils and Fats,2003, 28(11)：85-87)

［27］郭建,王梦凡,齐崴,等.响应面优化超声辅助提取大豆异黄酮联产分离蛋白和低聚糖［J］.大豆科学,2012,31(4):655-660(Guo J, Wang M F, Qi W, et al. Optimization of ultrasonicassisted extraction of soybean isoflavones by response surface methodology and simultaneous preparation of protein isolates and oligosaccharides ［J］.Soybean Science, 2012,31(4):655-660)

［28］左蕾蕾,刘文洁,曾凡骏.葛根综合利用与超声辅助提取葛根异黄酮工艺的研究［J］.食品与发酵科技,2011,47(4):60-63(Zuo L L, Liu W J, Zeng F J.The comprehensive utilization of pueraria and ultrasonic assisted extraction of isoflavones from pueraria residue［J］. Food and Fermentation Technology, 2011, 47(4):60-63)

［29］豆亚静，张晓龙，常丽新，等.响应面优化超声波法提取黑豆异黄酮的工艺研究［J］食品工业科技, 2013, 34(5):259-263(Dou Y J, Zhang X L, Chang L X, et al.Research of ultrasonic wave extraction of isoflavone from black soybean by response surface methodology［J］ Science and Technology of Food Industry, 2013, 34(5):259-263.

［30］刘军海,耿敬章.响应面法优化超声辅助提取豆粕中异黄酮工艺研究［J］.中国油脂,2013,38(4):72-74(Liu J H, Geng J Z. Optimization of ultrasonic-assisted extraction of isoflavone from soybean meal by response surface methodology［J］.China Oils and Fats,2013,38(4):72-74)

［31］李万林.豆粕中大豆异黄酮的超声辅助提取工艺研究［J］.皮革与化工,2014, 31(1):13-16(Li W L.Study on technology of ultrasound-assisted extracting for soy isoflavones in soybean meal［J］.Leather and Chemicals,2014,31(1):13-16)

［32］孙体健,王浩江,曹晓峰,等.发酵豆粕中大豆异黄酮的超声波提取工艺［J］.中国食品卫生杂志, 2006, 18(5):418-420 (Sun T J,Wang H J,Cao X F,et al.Study on conditions of ultrasonic extraction of soybean isoflavones from fermented soybean cake［J］.Chinese Journal of Food Hygiene, 2006, 18(5):418-420)

［33］史娟.豆渣中大豆异黄酮的超声提取研究［J］.江苏调味副食品,2010,27(4):10-13(Shi J.A research on extraction of soybean isoflavones from soybean residue by ultrasound［J］Jiangsu Condiment and Subsidiary Food,2010,27(4):10-13)

［34］刘建平,杨小敏,王雪芳,等.大豆豆渣中异黄酮的超声提取工艺研究［J］安徽农业科学,2010,38(4):2042-2043(Liu J P, Yang X M, Wang X F, et al.Study on the ultrasonic extraction technology of isoflavone from soybean residues［J］. Journal of Anhui Agriculture,2010,38(4):2042-2043)

［35］黄芸,崔力剑,窦玉红,等.中药淡豆豉异黄酮类化合物的超声循环提取［J］.精细化工, 2008,25(3):231-233(Huang Y,Cui L J,Dou Y H,et al.Ultrasonic and circulated extraction of total isoflavones from semen sojae praeparatum［J］.Fine Chemicals,2008,25(3):231-233)

［36］陈雅维，周惠云，王建普.纤维素酶解-超声偶联法提取葛根中总异黄酮的工艺优化［J］.天然产物研究与开发, 2012,24(7):933-938(Chen Y W,Zhou H Y,Wang J P.Cellulase coupling with ultrasound assisted extraction of total isoflavonoids from radix puerariae［J］.Natural Product Research and Development，2012, 24(7):933-938)

［37］李翠鸥,陈敬荣,郑尧杰,等.两相体系超声辅助酶水解法提取异黄酮苷元［J］.广东药学院学报, 2013,29(5):525-529(Li C O,Chen J R,Zheng Y J,et al.Study on extracting isoflavone aglycones with ultrasonic-assisted enzymatic hydrolysis in two-phase solvent system［J］.Journal of Guangdong Pharmaceutical University,2013,29(5):525-529)

［38］范蕴芳，胡碧纯，陈慧，等.超声-微波辅助提取葛根异黄酮工艺研究［J］.安徽农业科技，2013,41(12):5495-5497(Fan Y F,Hu B C,Chen H,et al.Study on the ultrasonic microwave assisted extraction technology of isoflavonoids from radix pueraria［J］.Journal of Anhui Agriculture, 2013,41(12):5495-5497)

［39］徐化能.葛藤异黄酮的浸取与分离过程研究［D］.杭州：浙江大学, 2007(Xu H N.Extraetion and separation of isoflavones from stem of Pueraria lobata (Wild) Ohwi ［D］Hangzhou: Zhe jiang University, 2007)

［40］张艳.大豆异黄酮测定方法的优化及大豆F3H基因沉默表达载体的构建［D］.吉林:吉林大学，2011(Zhang Y.The optimization of the determination of soybean isoflavones and soybean F3H gene silencing construction of expression vector ［D］.Jilin: Jilin University,2011)

［41］鞠兴荣，袁建，汪海峰.三波长紫外分光光度法测定大豆异黄酮含量的研究［J］. 食品科学，2001,22(5):46-48 (Ju X R, Yuan J, Wang H F. Studying on the determination of soybean isoflavone content by three wavelengths UV spectrophotometry［J］. Food Science, 2001,22(5):46-48)

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备注/Memo

基金项目：湖北工业大学大学生创新创业训练计划(201310500008)。

第一作者简介：汪美汐(1993-),女,主要从事药理学研究。E-mail: 18064070277@189.com。

717通讯作者：周宝晗(1980-)，女，博士，副教授，主要从事有机合成研究。E-mail: zhoubaohan@126.com。

更新日期/Last Update: 2015-09-01