[1]李相全,张忠亮,陶波,等.抗草甘膦转基因大豆对土壤酶活性及微生物数量的影响[J].大豆科学,2015,34(04):648-652.[doi:10.11861/j.issn.1000-9841.2015.04.0648]
　LI Xiang-quan,ZHANG Zhong-liang,TAO Bo,et al.Effects of Genetic Modified Glyphosate Resistant Soybean on Soil Enzyme Activity and Microorganisms[J].Soybean Science,2015,34(04):648-652.[doi:10.11861/j.issn.1000-9841.2015.04.0648]
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抗草甘膦转基因大豆对土壤酶活性及微生物数量的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年04期 页码: 648-652 栏目: 出版日期: 2015-08-25
Title:
Effects of Genetic Modified Glyphosate Resistant Soybean on Soil Enzyme Activity and Microorganisms
作者:
李相全1; 张忠亮1; 陶波1; 邱丽娟2
1.东北农业大学 农学院，黑龙江 哈尔滨 150030；
?2.中国农业科学院 作物科学研究所，北京 100081
Author(s):
LI Xiang-quan1;  ZHANG Zhong-liang1;  TAO Bo1;  QIU Li-juan2
1.College of Agriculture, Northeast Agricultural University, Harbin 150030, China;?
2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
关键词:
转基因大豆; 脲酶; 脱氢酶; 蔗糖酶; 微生物
Keywords:
Genetically modified soybean;  Urease;  Dehydrogenase;  Sucrase;  Microorganism
DOI:
10.11861/j.issn.1000-9841.2015.04.0648
文献标志码:
A
摘要:
利用生物学试验方法，对种植抗草甘膦转基因大豆呼交06-698及其非转基因亲本蒙豆12后的土壤酶活性及土壤微生物数量进行了测定，考察抗草甘膦转基因大豆对土壤生态环境的影响。结果表明：抗草甘膦转基因大豆呼交06-698与其亲本非转基因大豆蒙豆12在土壤酶活性和微生物数量两方面的变化趋势相似，不同时期土壤酶活性与微生物数量存在差异，同一时期抗草甘膦转基因大豆土壤脱氢酶、脲酶、蔗糖酶活性无显著性差异，土壤细菌数量差异不显著，仅在播种前和苗期抗草甘膦转基因大豆土壤真菌和放线菌数量显著高于非转基因大豆，其它时期土壤真菌和放线菌数量差异不显著。
Abstract:
The biological experiments was conducted to study soil ecological environment of Round Read soybean .aThe results showed that: the changes of enzyme activities and microbe quantity were roughly with the same trend in the soil of RRS Hujiao06-698 and its parent traditional soybean Mengdou 12. Soil enzyme activities and microbe quantity had certain difference in different growth stages Compared with non-transgenic soybean treatment, activities of soil enzymes had no significant difference in the same growth stage. And the amount of soil bacteria had also no significant difference in the same growth period. In pre-sowing and seedling stage, the soil fungi and actinomycete of RR soybean were significantly higher than those of cultural soybean.

参考文献/References:

［1］聂呈荣,王建武,骆世明.转基因植物对农业生物多样性的影响［J］. 应用生态学报,2003,14(8):1369-1373(Nie C R, Wang J W, Luo S M.Effect of transgenic plants on biodiversity of agroecosystem［J］. Chinese Journal of Applied Ecology, 2003,14(8):1369-1373)

［2］沈法富,韩秀兰,范术丽.转Bt-基因抗虫棉微生物区系和细菌生理群多样性的变化［J］ 生态学报,2004,24(3):432-437(Shen F F, Han X L, Fan S L. Changes in microbial flora and bacterial physiological group diversity in rhizosphere soil of transgenic Bt cotton［J］ Acta Ecologica Sinica, 2004,24(3):432-437)
［3］Romeis J, Battini M, Bigler F.Transgenic wheat with enhanced fungal resistance causes no effects Folsomia canadia (Collembola Isotomidae)［J］. Pedobiologia,2003,47:141-147
［4］范巧兰,陈耕,李永山,等.转Bt-基因棉花对土壤酶活性的影响［J］.农学学报,2013,3(1):32-35(Fan Q L, Chen G, Li Y S, et al. Effects of transgenic Bt cotton on soil enzymes［J］Journal of Agriculture, 2013,3(1):32-35)
［5］董莲华,孟盈,王晶. 转 Bt + CpTI 基因棉花对土壤细菌及氨氧化细菌数量的影响［J］. 微生物学报,2014,54(3):309-318(Dong L H, Meng Y, Wang J.Effects of transgenic Bt + CpTI cotton on rhizosphere bacteria and ammonia oxidizing bacteria population［J］ Acta Microbiologica Sinica, 2014,54(3):309-318)
［6］宋亚娜,苏军,陈睿,等转cry1Ac/cpti基因水稻对土壤酶活性和养分有效性的影响［J］ 生物安全学报,2011,20(3):243-248(Song Y N, Su J, Chen R, et al.Effect of cry1Ac/cpti- transgenic rice on enzyme activity and nutrient availability in paddy soil［J］Journal of Biosafety, 2011,20(3):243-248)
［7］陈晓雯,林胜,尤民生,等-转基因水稻对土壤微生物群落结构及功能的影响［J］.生物安全学报,2011,20(2):151-159(Chen X W, Lin S, You M S, at al. Effects of transgenic rice on the structure and function of soil microbial communities［J］. Journal of Biosafety, 2011,20(2):151-159)
［8］章秋艳,李刚,杨志国,等. 转基因大豆种植对根际土壤酶活性和养分的影响［J］. 中国油料作物学报,2014,36(3):409-413(Zhang Y Q, Li G, Yang Z G, et al. Effects of transgenic soybean on enzyme activities and nutrients in rhizosphere soil［J］. Chinese Journal of Oil Crop Sciences, 2014,36(3):409-413)
［9］陶波,蒋凌雪,沈晓峰,等.抗草甘膦转基因大豆对和非土壤酶活性的影响［J］.作物杂志,2011(1):45-49(Tao B, Jiang L X, Shen X F, at al. Effects of genetic modified glyphosate resistant soybean on soil enzyme es activities in rhizosphere and non-rhizosphere［J］.Crops, 2011(1):45-49)
［10］陶波,蒋凌雪,沈晓峰,等.抗草甘膦转基因大豆对和非土壤可培养菌的影响［J］. 东北农业大学学报,2011,42(1):10-16（Tao B, Jiang L X, Shen X F, at al.Effect of genetic modified glyphosate resistant soybean on soil culturable microorganisms in rhizosphere and non-rhizosphere［J］. Journal of Northeast Agricultural University, 2011,42(1):10-16）
［11］周洁,于崧,王珊珊,等.抗盐碱转基因大豆对根际土壤固氮细菌多样性的影响［J］. 大豆科学,2013,32(6):801-805(Zhou J, Yu S, Wang S S, et al.Effects of salinization resistance transgenic soybeans on rhizosphere soil nitrogen-fixing bacterial diversity［J］.Soybean Science, 2013,32(6):801-805)
［12］关松荫.土壤酶及其研究法［M］.北京:农业出版社, 1986:274-340(Guan S Y. Soil enzyme and its research method［M］. Beijing: Agricutural Press, 1986:274-340)
［13］Liu W, Lua H H, Wua W X, et al. Transgenic Bt rice does not affect enzyme activities and microbial composition in the rhizosphere during crop development［J］. Soil Biology and Biochemistry, 2008, 40: 475-486
［14］黄代中,肖文娟,刘云兵,等.浅水湖泊沉积物脱氢酶活性的测定及其生态学意义［J］. 湖泊科学,2009,21(3):345-350 (Huang D Z, Xiao W J, Liu Y B, at al. Determination of dehydrogenase activity in sediment of shallow lakes and its ecological significance［J］ Journal of Lake Science,2009,21(3):345-350)
［15］吴凡,林桂潮,吴坚文,等.转AtPAP15-基因大豆种植对土壤养分及酶活性的影响［J］. 土壤学报,2013,50(3):600-608(Wu F, Lin G C, Wu J W, at al. Effects of planting AtPAP15 transgenic on soil nutrients and enzyme activities in rhizosphere［J］Acta Pedologica Sinica, 2013,50(3):600-608)
［16］乔琦,丁伟,李新海,等.转基因抗旱大豆对土壤酶活性的影响［J］.东北农业大学学报,2010,41(12):11-14(Qiao Q, Ding W, Li X H, at al. Effect of transgenic drought resistant soybean on soil enzyme activity［J］. Journal of Northeast Agricultural University, 2010,41(12):11-14)
［17］刘佳,刘志华,徐广惠,等.抗草甘膦转基因大豆(RRS)对微生物和土壤氮素转化的影响［J］.农业环境科学学报,2010,29(7):1341-1345.（Liu J, Liu Z H, Xu G H, et al.Effects of roundup ready soybean（RRS）on microorganisms and nitrogen transformation in the rhizospheric soil［J］.Journal of AgroEnvironment Science, 2010,29(7):1341-1345）
［18］吕晓波,王宏燕,刘琦,等.抗草甘膦转基因大豆(RRS)在黑土生态系统种植的安全性研究［J］. 大豆科学,2009,28(2):260-265(Lyu X B, Wang H Y, Liu Q, et al. Biosafety of roundup ready soybean(RRS) planted in black soil ecosystem［J］. Soybean Science, 2009,28(2):260-265)
［19］袁红旭,张建中,郭建夫,等.种植转双价抗真菌基因水稻对微生物群落及酶活性的影响［J］土壤学报,2005,42(1):122-126(Yuan H X, Zhang J Z, Guo J F, at al. Activities of microbes and enzymes in soil after growing transgenic rice with two extra anti-fungus genes［J］Acta Pedologica Sinica, 2005,42(1):122-126)
［20］万小羽,梁永超,李忠佩,等.种植转Bt-基因棉对土壤生物活性的影响［J］.生态学报,2007,27(12):5414-5420(Wan X Y, Liang Y C, Li Z P, et al.Effect of planting transgenic Bt cotton on soil enzymatic and m icrobial activities［J］.Acta Ecologica sinica, 2007,27(12):5414-5420)
［21］Jepson P C, Croft B A, Pratt G E. Test systems to determine the ecological risks posed by toxin release from bacillus thuringiensis genes in crop plants［J］.Molecular Ecology,2008,3(1):81-89
［22］Oger P, Petit A, Dessaux Y.Genetically engineered plants producing opines alter their biological environment［J］. Nature Biotechnology, 1997, 15(4):369-372
［23］Northcott G L, Jones K C. Experimental approaches and analyticaltechniques for determining organic compound bound residues in soil and sediment［J］. Environmental Pollution, 2000, 108(1):19-43
［24］Kremer J R, Means E N, Kim S. Glyphosate affects soybeanroot exudation and rhizosphere micro-organisms［J］. International Journal of Environmental Analytical Chemistry,2005, 85(15):1165-1174.

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备注/Memo

基金项目：转基因生物新品种培育重大专项（2013ZX08004-001）。

第一作者简介：李相全（1989-），男，硕士，主要从事除草剂应用技术研究。E-mail：18646649654@163.com。
通讯作者：陶波（1963-），男，教授，博导，主要从事除草剂生物化学及应用技术。E-mail：botaol@163.com。

更新日期/Last Update: 2015-09-01