[1]阚贵珍,童振峰,胡振宾,等.野生大豆和抗草甘膦转基因大豆杂交后代的适合度分析[J].大豆科学,2015,34(02):177-184.[doi:10.11861/j.issn.1000-9841.2015.02.0177]
　KAN Gui-zhen,TONG Zhen-feng,HU Zhen-bin,et al.Fitness of Hybrids between Wild Soybeans (Glycine soja) and the Glyphosate-resistant Transgenic Soybean (Glycine max)[J].Soybean Science,2015,34(02):177-184.[doi:10.11861/j.issn.1000-9841.2015.02.0177]
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野生大豆和抗草甘膦转基因大豆杂交后代的适合度分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年02期 页码: 177-184 栏目: 出版日期: 2015-04-25
Title:
Fitness of Hybrids between Wild Soybeans (Glycine soja) and the Glyphosate-resistant Transgenic Soybean (Glycine max)
作者:
阚贵珍; 童振峰; 胡振宾; 马德元; 张国正; 喻德跃
南京农业大学 大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点实验室，江苏 南京 210095
Author(s):
KAN Gui-zhen;  TONG Zhen-feng;  HU Zhen-bin;  MA De-yuan;  ZHANG Guo-zheng;  YU De-yue
Soybean Research Institute of Nanjing Agricultural University, National Center for Soybean Improvement / National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China
关键词:
野生大豆; 抗草甘膦转基因大豆; 杂交后代; 适合度
Keywords:
Wild soybean;  Glyphosate-resistant transgenic soybean;  Hybrids;  Fitness
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.02.0177
文献标志码:
A
摘要:
野生大豆是大豆遗传改良的重要资源。转基因大豆可能对野生大豆资源存在潜在的农业和生态风险。外源基因从抗草甘膦转基因大豆向野生大豆材料的逃逸不仅需要成功的杂交，还要依赖于杂交后代的适合度。因此野生大豆和抗草甘膦转基因大豆杂交后代的适合度分析，对评价抗草甘膦转基因逃逸引起的生态风险非常必要。在网室条件下，4个野生大豆材料和抗草甘膦转基因大豆RR能够杂交结实，获得有抗草甘膦基因杂交后代群体F1和F2（江浦野生豆-5×RR）。对杂交后代及其母本野生大豆材料的7个农艺性状进行调查，计算适合度并进行t测验分析。结果表明：在没有草甘膦的选择压力下，杂交后代在一些性状上的相对适合度高于母本野生大豆材料；江浦野生豆-5和RR杂交F2代敏感株与抗性株在7个农艺性状有相对适合度上均差异不显著
Abstract:
Wild soybeans are important resources for soybean genetic improvement. The potential agricultural and ecological risk of transgenic soybeans may pose a threat to wild soybeans. Transgene escape from glyphosate-resistant, transgenic soybeans to wild soybeans also lies on the fitness of hybrid offspring except for successful cross. Therefore, analysis on the fitness of hybrids between wild soybeans and glyphosate-resistant transgenic soybeans is essential to assess environmental consequences caused by glyphosate-resistant transgene escape. In this study, F1?hybrids carrying the glyphosate resistance gene were obtained from crosses between four wild soybean and the glyphosate-resistant transgenic soybeans RR and reared under the net house conditions. Then, F2 .hybrids were derived from the cross between the wild soybean JPWS-5 and RR. Seven agronomic traits were measured in both hybrids and wild female soybeans. The relative fitness of the hybrids and wild females were analyzed by t-test. In the absence of glyphosate, we found that the hybrids between wild soybeans and RR had the relative fitness advantage of some traits to the wild female soybeans. The analysis on the fitness of F2?hybrids between JPWS5 and RR showed that there was no difference in the fitness for the seven agronomic traits between F2hybrids carrying the glyphosate resistance gene (F+2) and F2. hybrids without the glyphosate resistance gene (F-2). The results can provide scientific experimental data and theoretical support for safety assessment of transgenic soybeans and the protection of wild soybeans.

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备注/Memo

Foundation：Transgenic Breeding Program (2008ZX08004-003)

Biography：KAN Gui-zhen (1978-), female, PhD.Major in soybean molecular genetics and breeding. E-mail: kanguizhen@njau.edu.cn.
Corresponding author: YU De-yue (1965-), male, PhD, professor.Major in plant molecular genetics and breeding. E-mail: dyyu@njau.edu.cn.

更新日期/Last Update: 2015-06-07