[1]孟爽,赵洪锟,袁翠平,等.东北野生大豆核心种质单核苷酸多样性分析[J].大豆科学,2015,34(02):185-190.[doi:10.11861/j.issn.1000-9841.2015.02.0185]
　MENG Shuang,ZHAO Hong-kun,YUAN Cui-ping,et al.Genetic Diversity Analysis on SNP of Core Collection of Wild Soybean in Northeastern China.[J].Soybean Science,2015,34(02):185-190.[doi:10.11861/j.issn.1000-9841.2015.02.0185]
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东北野生大豆核心种质单核苷酸多样性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年02期 页码: 185-190 栏目: 出版日期: 2015-04-25
Title:
Genetic Diversity Analysis on SNP of Core Collection of Wild Soybean in Northeastern China.
作者:
孟爽1; 2; 赵洪锟2; 袁翠平2; 尹光3; 刘晓冬2; 王玉民2; 董英山2; 李建东1
1沈阳农业大学 农学院，辽宁 沈阳 110866；
?2吉林省农业科学院 农业生物技术研究所，吉林 长春 130033；
3深圳市高级中学，广东 深圳 518040
Author(s):
MENG Shuang1; 2;  ZHAO Hong-kun2;  YUAN Cui-ping2; YIN Guang3;  LIU Xiao-dong2;  WANG Yu-min2; ?DONG Ying-shan2;  LI Jian-dong1
1.College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China；
2.Agro-biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130033, China；
3.Shenzhen Sinior High School，Shenzhen 518040，China
关键词:
野生大豆; 核心种质; SNP; Indel; 遗传多样性
Keywords:
Wild soybean; Core collection; SNP; Indel; Genetic diversity
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.02.0185
文献标志码:
A
摘要:
对196个东北野生大豆核心种质的NRT2、AspAT2和CPK13三个基因片段进行单核苷酸扫描，共检测到94个SNPs位点及10个插入/缺失位点（Indel），碱基转换和颠换平均比例约2∶1。稀有SNPs位点（＜10%）39个，比率约为41.5%。196份核心种质平均多态性位点比例约为74%，Shannon′s指数是0.366，多样性指数为0.241；不同纬度群体的平均多样性指数随纬度下降逐渐升高，在44°N时达到峰值，然后随纬度降低缓慢下降，多样性指数变化呈近似正态曲线模式。初步推测N 42°～N45°区域为东北野生大豆的多样性中心。
Abstract:
One hundred and ninety-six wild soybean germplasm were selected from core collection of Northeastern China and the NRT2, AspAT2 and CPK13 gene sequences were used for SNP analysis. We detected 94 SNPs and 10 Indel with average proportion of nucleotide transitions and transversions of about 2∶1. There was 39 rare SNPs（＜10%）, with ratio of approximately 41.5%. The average proportion of polymorphic sites for 196 core collection was 74%, Shannon′s index of 0.366 and diversity index was 0.241. With the decline of latitude, average diversity index of populations of different latitudes was increased, reaching peak at 44°N. Changes in diversity index exhibited normal mode curve. It showed that the central diversity of wild soybean in Northeastern China was found at 42°N-45°N.

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备注/Memo

基金项目：国家自然科学基金（31300282）；吉林省科技厅应用基础项目（20130102050JC）；公益性行业（农业）科研专项（201003021）。

第一作者简介：孟爽（1988-），女，硕士，主要从事野生大豆遗传多样性研究。E-mail:mengshuang0518@126.com。通讯作者：李建东（1964-），男，教授，主要从事农业生态学和生物多样性研究。E-mail:sylijiandong@126.com；董英山（1963-），男，研究员，主要从事野生大豆资源研究。E-mail:ysdong@cjaas.com。

更新日期/Last Update: 2015-06-07