[1]任武贤,王星星,戴东升.纳豆激酶液态发酵工艺的优化[J].大豆科学,2015,34(01):128-130.[doi:10.11861/j.issn.1000-9841.2015.01.0128]
　REN Wu-xian,WANG Xing-xing,DAI Dong-sheng.Research on Liquid Fermentation Process of Nattokinase[J].Soybean Science,2015,34(01):128-130.[doi:10.11861/j.issn.1000-9841.2015.01.0128]
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纳豆激酶液态发酵工艺的优化

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年01期 页码: 128-130 栏目: 出版日期: 2015-02-25
Title:
Research on Liquid Fermentation Process of Nattokinase
作者:
任武贤; 王星星; 戴东升
亚宝药业集团股份有限公司, 山西 芮城 044600
Author(s):
REN Wu-xian; WANG Xing-xing; DAI Dong-sheng
Yabao Pharmaceutical Group Co. Ltd, Ruicheng 044600,China
关键词:
纳豆激酶; 液态发酵工艺; 碳源; 溶解氧
Keywords:
Nattokinases; Fermentation; Carbon source; Dissolved oxygen
分类号:
TQ920.6
DOI:
10.11861/j.issn.1000-9841.2015.01.0128
文献标志码:
A
摘要:
对由纳豆芽孢杆菌（Bacillus natto）液体摇瓶发酵生产纳豆激酶的发酵条件进行优化，并对5L发酵罐放大工艺进行研究。结果表明：以4% (w/v) 的可溶性淀粉作为碳源，种龄10 h，接种量3% (v/v)，通气量0.5 vvm，发酵罐转速由220 r?min-1提升至700 r?min-1，使发酵罐中溶解氧保持在20%以上，以上条件更有利于纳豆激酶的表达。条件优化后，摇瓶中纳豆激酶的表达量由低于1 000 IU?mL-1，提高到2 051.3 IU?mL-1（以尿激酶活为标准）。
Abstract:
The liquid fermentation process of nattokinase was studied both in shake flask experiment and 5L fermentor. The results showed that the best condition was 4% (w/v) soluble starch as carbon source, 10 h bacteria age, 3% (v/v) inoculum concentration, 0.5 vvm ventilation，and the rotate speed of fermentor increased from 220 r?min-1?to 700 r?min-1. All of those above could keep the dissolved oxygen in fermentor above 20%. After optimized, the expression quantity of Nattokinase in the shaker increased from lower than 1 000 IU?mL-1?to 2 051.3 IU?mL-1, equal to urokinase.

参考文献/References:

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备注/Memo

基金项目：山西省科技基础条件平台建设项目（2011091013）。

第一作者简介：任武贤（1960-），高级工程师，主要从事制药及药物研发研究。E-mail:renwuxian@yabaoyaoye.com。
通讯作者：戴东升（1972-），男，高级工程师，主要从事生物制药研究。E-mail:dongshengdai@126.com。

更新日期/Last Update: 2015-04-13