[1]李万林,钟姣姣,冯巩,等.大豆油中植物甾醇提取工艺及抗氧化活性研究[J].大豆科学,2014,33(05):731-734.[doi:10.11861/j.issn.1000-9841.2014.05.0731]
　LI Wan-lin,ZHONG Jiao-jiao,FENG Gong,et al.Extraction Process and Antioxidative Activity of Phytosterol in Soybean Oil[J].Soybean Science,2014,33(05):731-734.[doi:10.11861/j.issn.1000-9841.2014.05.0731]
点击复制

大豆油中植物甾醇提取工艺及抗氧化活性研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年05期 页码: 731-734 栏目: 出版日期: 2014-10-25
Title:
Extraction Process and Antioxidative Activity of Phytosterol in Soybean Oil
文章编号:
1000-9841.2014.05.0731
作者:
李万林1; 钟姣姣1; 冯巩1; 李波1; 赵红红1; 刘彩芬2; 李睿1; 刘欢1
1.陕西理工学院 化学与环境科学学院，陕西 汉中 723000;
2.陕西理工学院 生物科学与工程学院，陕西 汉中 723000
Author(s):
LI Wan-lin1; ZHONG Jiao-jiao1; FENG Gong1; LI Bo1; ZHAO Hong-hong1; LIU Cai-fen2; LI Rui1; LIU Huan1
1.College of Chemistry and Environmental Science,Shaanxi University of Technology，Hanzhong 723000，China;
2.College of Biological Science and Engineering,Shaanxi University of Technology，Hanzhong 723000,China
关键词:
大豆油; 植物甾醇; 微波; 提取工艺; 抗氧化性
Keywords:
Soybean oil; Phytosterol; Microwave; Extraction process; Antioxidative activity
分类号:
TS229
DOI:
10.11861/j.issn.1000-9841.2014.05.0731
文献标志码:
A
摘要:
以大豆油为原料，采用微波辅助提取植物甾醇，在单因素试验的基础上，通过正交试验对提取工艺进行优化。结果表明：微波辅助提取大豆油中植物甾醇最佳条件为：微波温度40℃、微波功率400 W、微波时间2 min、料液比1∶19(g∶mL），在此最佳工艺条件下，植物甾醇的提取量可达到2.317 mg.g-1。以大豆油中粗甾醇为原料，通过研究其清除羟自由基OH）的能力来确定其抗氧化性。结果表明，大豆油粗甾醇清除羟自由基(OH）的作用随质量浓度增加而增强，其IC50为16.430 mg.mL-1。
Abstract:
The extraction of phytosterol assisted by microwave was optimized through orthogonal experiments on the basis of single factor test by taking soybean oil as raw material.The results showed as follows:microwave temperature 40℃,microwave power 400 W,microwave time 2 min,ratio of material to liquid was 1 ∶19(g ∶mL),and under those conditions,the extraction volume could reach to 2.317 mg.g-1.The antioxidant was determined through studying the ability to scavenging hydroxyl free radicals(OH)of crude sterol which was extracted from soybean oil.The experimental results showed that,the ability of crude sterol scavenging hydroxyl free radical(OH)effect increasing with the concentration increasing,the IC50 was 16.430mg.mL-1.

参考文献/References:

［1］权静,卢定强,张筱.大豆功能性成分的研究现状[J].大豆通报,2004(3):27-29.(Quan J,Lu D Q,Zhang X.Recent development of soybean functional components[J].Soybean Bulletin,2004(3):27-29.)

[2]谢兵,傅相锴,隋岩,等.大豆天然甾醇的提取[J].西南师范大学学报,2005,30(5):897-900.(Xie B,Fu X K,Sui Y,et al.Extraction of soybean natural sterols[J].Journal of Southwest China Normal University,2005,30(5):897-900.)
[3]朱飞蛾,田自华,张子义.甘草总黄酮微波法提取工艺的研究[J].畜牧与饲料科学,2008(5):1-2.(Zhu F E,Tian Z H,Zhang Z Y.Licorice microwave extraction technology of total flavonoids[J].Animal Husbandry and Feed Science,2008(5):1-2.)
[4]刘海霞,王峰,赵雁武,等.苹果籽油中植物甾醇的提取及分光光度法含量测定研究[J].食品科学,2009,30(6):146-150.(Liu H X,Wang F,Zhao Y W,et al.Extraction and spectrophotometric determination of plant sterols in apple seed oil[J].Food Science,2009,30(6):146-150.)
[5]刘海霞,仇农学,王峰.苹果籽油中植物甾醇含量的薄层色谱―分光光度法测定[J].中国油脂,2008,33(11):76-79.(Liu H X,Qiu N X,Wang F.Phytosterols content in apple seed oil by thin-layer chromatography—spectrophotometric determination[J].China Oil,2008,33(11):76-79.)
[6]姚钰蓉.紫甘薯花青素的提取纯化、稳定性及抗氧化活性研究[D].保定:河北农业大学,2009.(Yao Y R.Study on extracting and purifying anthocyanin from purple sweet potato,stability and antioxidative activity[D].Baoding:Hebei Agricultural University,2009.)

相似文献/References:

[1]蔡 燕,周红波,朱金丽.低豆粕用量下脂肪氧合酶好氧催化大豆油的反应研究[J].大豆科学,2013,32(05):683.[doi:10.11861/j.issn.1000-9841.2013.05.0683]
　CAI Yan,ZHOU Hong-bo,ZHU Jin-li.Aerobic Catalyzed Lipoxygenation of Soybean Oil with Low Level Soymeal[J].Soybean Science,2013,32(05):683.[doi:10.11861/j.issn.1000-9841.2013.05.0683]
[2]李 川,万新军,任玉洁,等.固体碱K2CO3/NaX催化制备大豆生物柴油的研究[J].大豆科学,2014,33(01):110.[doi:10.11861/j.issn.1000-9841.2014.01.0110]
　LI Chuan,WAN Xin-jun,REN Yu-jie,et al.Preparation of the Soybean Biodiesel by K2CO3/NaX Solid Base[J].Soybean Science,2014,33(05):110.[doi:10.11861/j.issn.1000-9841.2014.01.0110]
[3]黄艳芹.新型固体碱催化大豆油制备生物柴油的工艺研究[J].大豆科学,2011,30(04):668.[doi:10.11861/j.issn.1000-9841.2011.04.0668]
　HUANG Yan-qin.Preparation of Biodiesel from Soybean Oil Catalyzed by New Solid Basic Catalyst[J].Soybean Science,2011,30(05):668.[doi:10.11861/j.issn.1000-9841.2011.04.0668]
[4]张新海,张守花.大豆油酯化催化剂负载型K2O固体碱的制备及性能研究[J].大豆科学,2011,30(06):1027.[doi:10.11861/j.issn.1000-9841.2011.06.1027]
　ZHANG Xin-hai,ZHANG Shou-hua.Preparation and Performance of Soybaen Oil Esterification Catalyst with Supported Solid Base K2O[J].Soybean Science,2011,30(05):1027.[doi:10.11861/j.issn.1000-9841.2011.06.1027]
[5]张守花,张新海,张洪浩.K2O/Ti-HMS固体碱催化大豆油制备生物柴油[J].大豆科学,2010,29(06):1043.[doi:10.11861/j.issn.1000-9841.2010.06.1043]
　ZHANG Shou-hua,ZHANG Xin-hai,ZHANG Hong-hao.K2O/Ti-HMS Solid Base of Soybean Oil Catalyzed Biodiesel Oil[J].Soybean Science,2010,29(05):1043.[doi:10.11861/j.issn.1000-9841.2010.06.1043]
[6]李红,汪洋,李刚.碱催化下大豆生物柴油的制备研究[J].大豆科学,2010,29(06):1052.[doi:10.11861/j.issn.1000-9841.2010.06.1052]
　LI Hong,WANG Yang,LI Gang.Preparation of the Soybean Biodiesel Catalyzed by Alkali[J].Soybean Science,2010,29(05):1052.[doi:10.11861/j.issn.1000-9841.2010.06.1052]
[7]孙玉秋,陈波水,方建华,等.大豆油及其生物柴油低温流动特性研究[J].大豆科学,2009,28(02):314.[doi:10.11861/j.issn.1000-9841.2009.02.0314]
　SUN Yu-qiu,CHEN Bo-shui,FANG Jian-hua,et al.Cold Flow Properties of Soybean Oil and Its Biodiesel[J].Soybean Science,2009,28(05):314.[doi:10.11861/j.issn.1000-9841.2009.02.0314]
[8]曾红舟,蒋惠亮,郭宏珍.强碱催化大豆油酯交换制备生物柴油[J].大豆科学,2007,26(04):583.[doi:10.3969/j.issn.1000-9841.2007.04.025]
　ZENG Hong-zhou,JIANG Hui-liang,GUO Hong-zhen.ALKALI-CATALYZED TRANSESTERIFICATION OF SOYBEAN OIL FOR PRODUCTION OF BIODIESEL[J].Soybean Science,2007,26(05):583.[doi:10.3969/j.issn.1000-9841.2007.04.025]
[9]曹秀丽,王赛丹,李肖,等.响应面试验优化大豆油甲酯合成工艺[J].大豆科学,2017,36(01):124.[doi:10.11861/j.issn.1000-9841.2017.01.0124]
　CAO Xiu-li,WANG Sai-dan,LI Xiao,et al.Optimal Synthesis of Soybean Oil Methyl Ester by Response Surface Methodology[J].Soybean Science,2017,36(05):124.[doi:10.11861/j.issn.1000-9841.2017.01.0124]

备注/Memo

基金项目：陕西省2014年省级大学生创新创业训练项目（1594）；陕西理工学院大学生创新实验项目（UIRP2014012）。

第一作者简介：李万林（1991-），男，学士，主要从事天然产物的提取、分离和纯化研究。E-mail：18049165510@163.com。

更新日期/Last Update: 2014-12-27