[1]李小平,曾庆发,赵娟.大豆生长素响应因子GmARF16器官表达特征及抗降解表达载体的构建[J].大豆科学,2014,33(05):661-666.[doi:10.11861/j.issn.1000-9841.2014.05.0661]
　LI Xiao-ping,ZEN Qing-fa,ZHAO Juan.Expression Pattern of GmARF16 in Different Soybean Organs and Construction of Resistant Version Vector for GmARF16[J].Soybean Science,2014,33(05):661-666.[doi:10.11861/j.issn.1000-9841.2014.05.0661]
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大豆生长素响应因子GmARF16器官表达特征及抗降解表达载体的构建

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年05期 页码: 661-666 栏目: 出版日期: 2014-10-25
Title:
Expression Pattern of GmARF16 in Different Soybean Organs and Construction of Resistant Version Vector for GmARF16
文章编号:
1000-9841.2014.05.0661
作者:
李小平; 曾庆发; 赵娟
淮北师范大学 生命科学学院/安徽省资源植物重点实验室，安徽 淮北 235000
Author(s):
LI Xiao-ping; ZEN Qing-fa; ZHAO Juan
College of Life Sciences,Huaibei Normal University/Anhui Plant Resource Key Lab,Huaibei,235000,China
关键词:
生长素响应因子; 构建; MicroRNA; 表达分析
Keywords:
Auxin response factors; Construction; MicroRNA; Expression analysis
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0661
文献标志码:
A
摘要:
通过挖掘大豆基因组信息并参考拟南芥同源基因序列，分析了大豆(Glycine max)的生长素响应因子GmARF16和Gm-miR160作用位点，设计出mGmARF16序列并成功构建了pGmARF16∷mGmARF16双元表达载体。此外，通过实时荧光定量PCR(qPCR)分析了GmARF16表达模式，发现该基因在大豆根、茎、叶、花、果荚和种子中均有一定量的表达，其中花中表达最强，茎中表达最弱；复叶中表达量高于子叶和第一对真叶。
Abstract:
By mining soybean and Arabidopsis genome data,we analyzed the interaction between auxin response factor GmARF16 and Gm-miR160,a kind of the microRNA and generated resistant version fragment mGmARF16.To looking for function of GmARF16,a binary vector pGmARF16∷mGmARF16 was constructed using mGmARF16 through Topo cloning and LR reaction.Moreover,real-time quantity PCR was used to investigate expression pattern of GmARF16 throughout the life span of soybean.GmARF16 was found to be -expressed at high levels in flowers and at low levels in stem.Interestingly,its transcriptional level was found higher in trifoliolate than either in cotyledons or in unifoliolate.

参考文献/References:

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备注/Memo

基金项目：国家自然科学基金（30970245）。

第一作者简介：李小平（1972-），男，博士，副教授，主要从事植物分子生物学研究。E-mail：1244232074@qq.com。
通讯作者：赵娟（1978-），女，硕士，副教授，主要从事分子遗传学研究。E-mail:1540912306@qq.com。

更新日期/Last Update: 2014-12-25