[1]易 嘉,郭传龙,武孔焕,等.壳梭孢素对铝敏感型黑大豆铝耐受性的影响[J].大豆科学,2014,33(03):389-392.[doi:10.11861/j.issn.1000-9841.2014.03.0389]
　YI Jia,GUO Chuan-long,WU Kong-huan,et al.Effects of Fusicoccin on Aluminum Tolerance in Alsensitive Black Soybean[J].Soybean Science,2014,33(03):389-392.[doi:10.11861/j.issn.1000-9841.2014.03.0389]
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壳梭孢素对铝敏感型黑大豆铝耐受性的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年03期 页码: 389-392 栏目: 出版日期: 2014-06-25
Title:
Effects of Fusicoccin on Aluminum Tolerance in Alsensitive Black Soybean
作者:
易 嘉; 郭传龙; 武孔焕; 王 琳; 赵秀玲; 李昆志; 陈丽梅
昆明理工大学 生物工程技术研究中心，云南 昆明 650500
Author(s):
YI Jia; GUO Chuan-long; WU Kong-huan; WANG Lin; ZHAO Xiu-ling; LI Kui-zhi; CHEN Li-mei
Biotechnology Research Centre,Kunming University of Science and Technology,Kunming 650500,China
关键词:
黑大豆; 14-3-3蛋白; 质膜H+-ATPase; 壳梭孢素; 铝胁迫
Keywords:
Black soybean; 14-3-3 proteins; Plasma membrane H+ATPase; Fusicoccin; Al stress
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.03.0389
文献标志码:
A
摘要:
以铝敏感型黑大豆（简称SB）为材料，研究铝胁迫下壳梭孢素（FC）对SB根铝耐受性的影响。结果表明：在50 μmol.L-1铝胁迫下，添加1 μmo.L-1 FC处理8 h能够显著缓解铝胁迫对SB根生长的抑制作用，使其根的相对生长率（RRG）增加36.4%。免疫共沉淀分析结果表明50 μmol.L-1铝胁迫下添加1 μmo.L-1FC增强SB根中14-3-3蛋白与磷酸化质膜H+- ATPase的结合，使根尖质膜H+-ATPase活性提高约1倍，根氢泵活性和质子外排氢离子能力显著增强，根尖柠檬酸的分泌量增加显著，增加约2倍。这些结果证实铝胁迫下FC用于增强SB根中14-3-3蛋白和质膜H+-ATPase的相互作用和质膜H+-ATPase的活性，从而增加SB根柠檬酸的分泌作用及其对铝胁迫的耐受性。
Abstract:
Effect of FC on aluminum tolerance of Alsensitive black soybean(SB)roots under Al stress were investigated in this research.The results showed that adding 1 μmol.L-1FC for 8 h,it could significantly release the inhibition of 50 μmol.L-1Al stress on the root growth of SB,and increase its relative root growth(RRG)by 36.4%.CoImmunoprecipitation(Co-IP)analysis showed that adding 1 μmol.L-1FC could enhance the interaction between 14-3-3 proteins and phosphorylated PM H+-ATPase,which increased PM H+-ATPase activity to approximately 1 fold,significantly increasing proton efflux and citrate secretion to approximately 2fold in SB roots under 50 μmol.L-1 Al stress.These results confirmed FC was used for enhancing interaction of the 14-3-3 protein and PM H+-ATPase and the activity of PM H+-ATPase,thereby increasing citrate secretion and Altolerance in SB roots under Al stress.

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备注/Memo

收稿日期：2013-11-06

基金项目：国家重点基础研究发展计划“973计划”(2007CB108901)。第一作者简介：易嘉(1985-)，男，硕士，主要从事植物代谢途径基因工程方面的研究。E-mail:yijiad013@163.com。
通讯作者：陈丽梅(1963-)，女,教授，主要从事植物代谢途径基因工程方面的研究。E-mail:chenlimeikm@126.com。

更新日期/Last Update: 2014-08-01