[1]郭传龙,赵艳,武孔焕,等.AMP对铝胁迫诱导丹波黑大豆柠檬酸分泌及其铝耐受性的影响[J].大豆科学,2015,34(01):82-86.[doi:10.11861/j.issn.1000-9841.2015.01.0082]
　GUO Chuan-long,ZHAO Yan,WU Kong-huan,et al.Effects of AMP on the Al-induced Citrate Exudation and Al-resistance of Tamba Black Soybean[J].Soybean Science,2015,34(01):82-86.[doi:10.11861/j.issn.1000-9841.2015.01.0082]
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AMP对铝胁迫诱导丹波黑大豆柠檬酸分泌及其铝耐受性的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年01期 页码: 82-86 栏目: 出版日期: 2015-02-25
Title:
Effects of AMP on the Al-induced Citrate Exudation and Al-resistance of Tamba Black Soybean
作者:
郭传龙; 赵艳; 武孔焕; 李昆志; 陈丽梅
昆明理工大学 生命科学与技术学院生物工程技术研究中心，云南 昆明 650500
Author(s):
GUO Chuan-long;  ZHAO Yan;  WU Kong-huan;  LI Kun-zhi;  CHEN Li-mei
Biotechnology Research Centre, Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Kunming 650500, China
关键词:
铝胁迫; 丹波黑大豆; 铝诱导的柠檬酸分泌; 14-3-3蛋白; 质膜H+ATP酶
Keywords:
Al stress;  Al resistant black soybean;  14-3-3 protein; Plasma membrane H+-ATPase; Adenosine 5’-monophosphate
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.01.0082
文献标志码:
A
摘要:
为了考察AMP（Adenosine 5’ monophosphate）对铝耐受型丹波黑大豆（RB）柠檬酸的分泌及铝抗性的影响，在50 mmol?L-1铝溶液中添加100 mmol?L-1?AMP共处理RB，结果表明RB根的相对生长率显著小于单独用50 mmol?L-1铝胁迫处理的植株。免疫共沉淀分析显示铝胁迫下AMP的存在降低RB根中14-3-3蛋白与磷酸化质膜H+ATP酶的相互作用，使根尖质膜H+ATP酶活性降低约1倍，氢泵活性和H+分泌能力显著降低，根柠檬酸的分泌量减少约1倍。说明铝胁迫下AMP通过抑制14-3-3蛋白和质膜H+ATP酶的互作来减少RB根尖的氢泵活性和柠檬酸分泌作用，从而降低RB根的相对生长率及其对铝胁迫的耐受性。
Abstract:
To investigate the effect of Adenosine 5’ monophosphate (AMP) on the citrate secretion and Alresistance of Alresistant Tamba black soybean (RB). RB was cotreated with AMP and 50 mmol?L-1?Al. The results showed that the relate root growth rate of RB cotreated with 100 mmol?L-1?AMP and 50 mmol?L-1?Al for 8 h was significantly lower than that of the plants treated with 50 mmol?L-1?Al alone. Co-immunoprecipitation showed that the presence of AMP under Al stress decreased the Al-enhanced interaction of PM H+-ATPase and 14-3-3 protein in root tips of RB, which reduced PM H+-ATPase activity by approximately 1-fold and thereby led to a significant decrease in H+ efflux and citrate secretion (approximately 1-fold) in RB roots. Those results indicated that AMP decreases H+ activity and citrate exudation in RB via its inhibition on the interaction between 14-3-3 protein and PM H+-ATPase under Al stress, thereby reducing the relate root growth rate of RB and its Al-tolerance.

参考文献/References:

［1］Ma J F, Furukawa J. Recent progress in the research of external Al detoxification in higher plants:A minireview ［J］. Journal of Inorganic Biochemistry, 2003, 97:46-51.

［2］Ohno T, Koyama H, Hara T. Characterization of citrate transport through the plasma membrane in a carrot mutant cell line with enhanced citrate excretion ［J］. Plant Cell Physiology, 2003, 44 (2):156-162.
［3］Shen H, He L, Sasaki T, et al. Citrate secretion coupled with the modulation of soybean root tip under aluminum stress:Up-regulation of transcription, translation, and threonine-oriented phosphorylation, of plasma membrane H+-ATPase ［J］. Plant Physiology, 2005, 138:287-296.
［4］Kim Y S, Park W, Nian H, et al. Aluminum tolerance associated with enhancement of plasma membrane H+-ATPase in the root apex of soybean ［J］. Soil Science & Plant Nutrition, 2010, 56 (1):140-149.
［5］Sehnke P C, DeLille J M, Ferl R J. Consummating signal transduction: the role of 14-3-3 proteins in the completion of signal-induced transitions in protein activity ［J］. Plant Cell, 2002, 14 (Sup.): 339–354.
［6］Guo C L, Chen Q, Chen X Q, et al. Al-enhanced expression and interaction of 14-3-3 protein and plasma membrane H+-ATPase is related to Al-induced citrate secretion in an Al-resistant black soybean ［J］. Plant Molecular Biology Reporter, 2013, 31(4) DOI:10.1007/s11105-013-0569-0.?
［7］Chen Q, Guo C L, Wang P, et al. Up-regulation and interaction of the plasma membrane H+-ATPase and the 14-3-3 protein are involved in the regulation of citrate exudation from the broad bean (Vicia faba L.) under Al stress ［J］. Plant Physiology and Biochemistry, 2013, 70:504-511.
［8］Camoni L, Visconti S, Marra M, et al. Adenosine 5’-Monophosphate inhibits the association of 14-3-3 protein with the plant plasma membrane H+-ATPase ［J］. Journal of Biological Chemistry, 2001, 276(34):31709-31712.
［9］Toroser D, Athwal G S, Huber S C. Site-specific regulatory interaction between spinach leaf sucrose-phosphate synthase and 14-3-3 proteins ［J］. FEBS Letters, 1998, 435(1): 110-114.
［10］Paul A L, Sehnke P C, Ferl R J. Isoform-specific subcellular localization among 14-3-3 proteins in Arabidopsis seems to be driven by client interactions ［J］. Molecular Biology of the Cell, 2005, 16:1735-1743.
［11］Zhao R, Dielen V, Kinet J M, et al. Cosuppression of a plasma membrane H+-ATPase isoform impairs sucrose translocation, stomatal opening, plant growth, and male fertility ［J］. Plant Cell, 2000, 12(4):535-546.
［12］Ligaba A, Yamaguchi M, Shen H, et al. Phosphorus deficiency enhances plasma membrane H+-ATPase activity and citrate exudation in greater purple lupin (Lupinus pilosus) ［J］. Function Plant Biology,2004, 31(11):1075-1083.
［13］Ma gorzata J R. Plant plasma membrane H+-ATPase in adaptation of plants to abiotic stresses［M］//Shanker A, Venkateswarlu B. Abiotic stress response in plants-physiological, biochemical and genetic perspectives. Rijeka, Croatia: InTech, 2011, 197-218.

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备注/Memo

基金项目：国家自然科学基金(31260063)。

第一作者简介：郭传龙（1987-），男，硕士，主要从事环境植物分子生物学方面的研究。E-mail:gclong123@126.com。
通讯作者：陈丽梅（1963-），女，教授，主要从事环境植物分子生物学方面的研究。E-mail:chenlimeikm@126.com。

更新日期/Last Update: 2015-04-12