[1]赵莉莉,李喜焕,李文龙,等.MYB转录因子 AtPHR1 转化大豆研究[J].大豆科学,2013,32(02):154-159.[doi:10.3969/j.issn.1000-9841.2013.02.004]
　ZHAO Li-li,LI Xi-huan,LI Wen-long,et al.Genetic Transformation of Transcription Factor AtPHR1 into Soybean by Agrobacterium-Mediated Method[J].Soybean Science,2013,32(02):154-159.[doi:10.3969/j.issn.1000-9841.2013.02.004]
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MYB转录因子 AtPHR1 转化大豆研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年02期 页码: 154-159 栏目: 出版日期: 2013-04-25
Title:
Genetic Transformation of Transcription Factor AtPHR1 into Soybean by Agrobacterium-Mediated Method
作者:
赵莉莉; 李喜焕; 李文龙; 常文锁; 张彩英
（河北农业大学 /教育部华北作物种质资源研究与利用重点实验室，河北 保定 071001）
Author(s):
ZHAO Li-li; LI Xi-huan; LI Wen-long; CHANG Wen-suo; ZHANG Cai-ying
关键词:
MYB转录因子; 农杆菌介导; 大豆; 遗传转化
Keywords:
MYB transcription factor; Agrobacterium-mediated method; Soybean; Genetic transformation
DOI:
10.3969/j.issn.1000-9841.2013.02.004
文献标志码:
A
摘要:
以MYB转录因子AtPHR1表达载体为转化对象，采用农杆菌介导子叶节转化技术将其转入栽培大豆,研究不同萌发时间、预培养时间、菌液和侵染液浓度、大豆基因型以及筛选剂浓度对转化效率的影响。结果表明:以萌发5 d大豆幼苗制备子叶节外植体，预培养1 d，农杆菌菌液OD600 为0.7，侵染液OD600 为0.5或0.7进行转化，100 mg·L-1 卡那霉素进行筛选的抗性芽诱导率最高；5个供试品种中，以五星2号的转化率最高，约为2.0%；经PCR检测转化后的大豆植株，获得了含有转录因子AtPHR1的5个大豆新材料。
Abstract:
In this study，a MYB transcription factor AtPHR1 was introduced into soybean varieties by Agrobacterium-mediated cotyledonary node transformation method to study the influence factors on transformation efficiency.The studied factors included seedling age，preincubation period，strain density，infectious concentration，kanamycin concentration and soybean genotype.The results showed that a much higher induction rate of resistant shoots was obtained when the seedling age was 5 d from germination with 1 d pre-culture，the strain density(OD600)was 0.7 and infectious concentration was 0.5 or 0.7，and also the kanamycin concentration was 100 mg·L-1.The soybean variety，Wuxing 2，with the highest transformation efficiency was about 2.0%.Finally，five new PCR positive transgenic materials with transcription factor AtPHR1 were gained.

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备注/Memo

?农业科技成果转化资金（2012GB2A200032）；国家转基因重大专项（2009ZX08004-004B）；河北省自然科学基金（C2010000749）。

更新日期/Last Update: 2014-01-23