[1]刘淼,李冬梅,王志坤,等.转ThIPK2基因大豆的农艺性状及光合生理研究[J].大豆科学,2012,31(05):775-777.[doi:10.3969/j.issn.1000-9841.2012.05.018]
　LIU Miao,LI Dong-mei,WANG Zhi-kun,et al.Agronomic Traits and Photosynthetic Traits of Transgenic Soybean Overexpressing ThIPK2[J].Soybean Science,2012,31(05):775-777.[doi:10.3969/j.issn.1000-9841.2012.05.018]
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转ThIPK2基因大豆的农艺性状及光合生理研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年05期 页码: 775-777 栏目: 出版日期: 2012-10-25
Title:
Agronomic Traits and Photosynthetic Traits of Transgenic Soybean Overexpressing ThIPK2
文章编号:
1000-9841（2012）05-0775-03
作者:
刘淼1; 2; 李冬梅1; 王志坤1; 孟凡立1; 李永光1; 武小霞1; 张洪霞3; 李文滨1
1.大豆生物学教育部重点实验室，农业部大豆生物学与遗传育种北方区域重点实验室，东北农业大学 大豆研究所，黑龙江 哈尔滨 150030；
2.黑龙江省农业科学院 耕作栽培研究所，黑龙江 哈尔滨 150086；
3.中国科学院 上海生命科学研究院 植物生理生态研究所，上海 200032
Author(s):
LIU Miao1; 2; LI Dong-mei1; WANG Zhi-kun1; MENG Fan-li1; LI Yong-guang1; WU Xiao-xia1; ZHANG Hong-xia3; LI Wen-bin1
1. Breeding for Soybean in Northeast China,Ministry of Agriculture,Soybean Research Institute,Northeast Agricultural University,Harbin 150030,Heilongjiang;
2.Crop Tillage and Cultivation Institute,Heilongjiang Academy of Agricultural Sciemes,Harbin 150086,Heilongjiang;
3.National Key Laboratory of Plant Molecular Genetics,Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China
关键词:
ThIPK2; 转基因大豆; 抗逆; 生育期; 农艺性状
Keywords:
ThIPK2; Transgenic soybean; Stress-tolerance; Growth and development period; Agronomic trait
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.05.018
文献标志码:
A
摘要:
将抗逆基因ThIPK2转入东农50，得到稳定遗传株系，将T4代株系种子正常播种，将转基因植株与对照东农50的生育期、株高、主茎节数、分枝数、主茎荚数、分枝荚数、单株粒数等性状进行比较，研究ThIPK2基因对大豆农艺性状方面的影响。结果表明，在正常条件下，转基因株系和对照植株相比，生育期并没有改变，而株高、主茎荚数、分枝荚数、分枝数和单株粒数农艺性状呈显著或极显著提高，这表明ThIPK2基因对提高大豆产量有利。干旱胁迫导致大豆叶片各项光合生理指标下降；但转ThIPK2基因大豆的各项光合生理指标高于非转基因对照。经干旱胁迫后，转基因植株与对照相比积累了更多的干物质。
Abstract:
Dongnong50 was transformed with ThIPK2,a stress-tolerance gene,and transgenic progenies with ThIPK2 with stable heredity characters were obtained in this study.The seeds of T4 generation were planted on transgenic experimental base,investigated agronomic traits and compared to control plants Dongnong50.For the transgenic plants,the growth duration remained stable,while significant increase were observed for plant height,pods on main stem,pods on branch,and seeds per plant.Hence, the ThIPK2 gene is beneficial to improve soybean yields.Drought stress declined all indicators of photosynthetic physiology.But indicators of transgenic soybean were higher than non-transgenic soybean under drought stress.In addition,transgenic plants accumulated more dry matter than controls under drought condition.

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备注/Memo

基金项目：国家转基因重大专项（2011ZX08004-002）；农业部大豆产业技术体系项目（CARS-04-PS04）。

第一作者简介：刘淼(1983-)，女，在读博士，研究方向为大豆基因工程。E-mail:185661659@qq.com。?
通讯作者：李文滨（1958-），男，教授，博士生导师，主要从事大豆遗传育种研究。E-mail：wenbinli@neau.edu.cn

更新日期/Last Update: 2014-08-19