[1]芦春斌,金庆敏,杨冬宇.广州市农贸市场中转基因大豆的检测[J].大豆科学,2012,31(04):680-684.[doi:10.3969/j.issn.1000-9841.2012.04.034]
　LU Chun-bin,JIN Qing-min,YANG Dong-yu.Detection of the Roundup Ready Soybean in the Market of Guangzhou[J].Soybean Science,2012,31(04):680-684.[doi:10.3969/j.issn.1000-9841.2012.04.034]
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广州市农贸市场中转基因大豆的检测

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年04期 页码: 680-684 栏目: 出版日期: 2012-08-25
Title:
Detection of the Roundup Ready Soybean in the Market of Guangzhou
文章编号:
1000-9841（2012）04-0680-05
作者:
芦春斌; 金庆敏; 杨冬宇
暨南大学 生命科学技术学院 生殖免疫研究所,广东 广州 510632
Author(s):
LU Chun-bin; JIN Qing-min; YANG Dong-yu
Institute of Reproductive Immunology,College of Life Science and Technology,Jinan University,Guangzhou 510632,Guangdong,China
关键词:
转基因大豆; 检测; 聚合酶链式反应
Keywords:
Transgenic soybean; Detection; PCR
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.04.034
文献标志码:
A
摘要:
对广州市农贸市场中销售的大豆进行转基因大豆的初步筛查，以检测在广州市场是否有转基因大豆流通和销售，为相关标识和监管提供实验依据。根据转基因大豆内参凝集素Lectin基因及外源基因CaMV35S、NOS及EPSPS基因序列设计4对引物，对收集的大豆样品提取其基因组DNA后进行PCR检测。结果在所收集的14份大豆样品中检测出1份转基因大豆，表明在广州农贸市场中存在转基因大豆，并在市场流通，提醒相关部门需根据国家规定需要加强监管力度。
Abstract:
In recent years,with the development of transgenic technology,more and more transgenic plants have been marketed.Among genetically modified plants,the planting area and yield of transgenic soybean ranked in the top in the world.GM soybean has also become the largest import crop in China.This study was aimed to monitor the presence of transgenic soybean in the market of Guangzhou city.In Guangzhou market,circulating soybeans were sampled to detect genetically modified soybeans.Four primer pairs of EPSPS,lectin,CaMV35S promoter and NOS terminator targeted the sequence of foreign genes and reference gene,Lectin，were designed.Genomic DNA of soybean samples were extracted and amplified by PCR,and the detection results showed the presence of one line of transgenic soybean among 14 samples.Therefore,it was strongly suggested that the supervision of circulation and sale of soybean in free-market must be strengthened by governmental reinforcement followed governmental regulations.

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备注/Memo

基金项目：转基因生物新品种培育项目（2011ZX08012-005）；广东省科技计划项目（2010B031600108）。

第一作者简介：芦春斌(1964-），男，副研究员, 博士，硕士生导师， 研究方向为转基因与生物安全。E-mail:tcblu@jnu.edu.cn。

更新日期/Last Update: 2014-08-19