GAO Yuan,CHANG Wei,HAN Ying-peng,et al.Comparisons and Analyses on the Two Methods of Evaluating Resistance to Soybean Cyst Nematode[J].Soybean Science,2012,31(02):274-277.[doi:10.3969/j.issn.1000-9841.2012.02.023]
2种大豆胞囊线虫鉴定方法比较及分析
- Title:
- Comparisons and Analyses on the Two Methods of Evaluating Resistance to Soybean Cyst Nematode
- 文章编号:
- 1000-9841（2012）02-0274-04
- Keywords:
- Soybean Cyst Nematode; Resistance evaluation; Plastic column method; Acid magenta dyeing method
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 从计数方法、播种方法、鉴定准确性等方面对实用性较强的塑料钵柱法和酸性品红染色法2种大豆胞囊线虫鉴定方法进行了比较。塑料钵柱法鉴定时，可观察到大豆胞囊线虫病的主要发病特征，包括叶片变黄﹑植株矮小﹑根部有胞囊形成等典型症状。利用酸性品红染色法鉴定时未见叶片变黄，根部未见胞囊，但在20×显微镜下可检测处于二龄期的幼虫，可用于鉴定计数。2种鉴定方法对鉴别寄主具有同等区别抗感材料的能力，但在鉴别较大群体时品红染色法鉴定结果变异率低，同时在鉴定周期和效率上明显优于塑料钵柱法。因此，在对较大群体进行鉴定时，酸性品红染色法是一种便捷且准确性较高的鉴定方法。
- Abstract:
- The difference of two identification method of soybean cyst nematode including plastic column method and acid magenta dyeing method were compared from aspects of cyst counting methods，soybean planting method，and identification accuracy.For plastic column method,the typical symptom of infected plants including yellowed leaves,dwarfed plants and formation of cyst in root could be seen directly.For acid magenta dyeing method,the number of second-stage juveniles (J2) of SCN could be counted under the 20×microscope,while the morphological variation couldn’t be seen.The two methods had the same ability to identify resistant and susceptible materials,while acid magenta dyeing method showed higher efficiency and accuracy.Results suggest when identifying SCN resistance for larger soybean population,acid magenta dyeing method is suitable for its time-saving and high-efficiency characteristics.
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备注/Memo
基金项目：国家高技术研究发展计划（863计划）项目（2006AA100104-4）；国家重点基础研究发展计划（973计划）项目(2009CB118400)；黑龙江省教育厅面上项目（11541025）；哈尔滨市科技创新人才研究专项（2009RFQXN085）。