[1]毕影东,李 炜,林 红,等.大豆种间杂交异黄酮含量的杂种优势分析[J].大豆科学,2012,31(02):203-207.[doi:10.3969/j.issn.1000-9841.2012.02.009]
　BI Ying-dong,LI Wei,LIN Hong,et al.Heterosis of Soybean Isoflavone Content in Interspecific Hybridization[J].Soybean Science,2012,31(02):203-207.[doi:10.3969/j.issn.1000-9841.2012.02.009]
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大豆种间杂交异黄酮含量的杂种优势分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年02期 页码: 203-207 栏目: 出版日期: 2012-04-25
Title:
Heterosis of Soybean Isoflavone Content in Interspecific Hybridization
文章编号:
1000-9841（2012）02-0203-05
作者:
毕影东1; 李 炜1; 林 红2; 肖佳雷1; 李 琬1; 杨雪峰2; 来永才1
1.黑龙江省农业科学院 耕作栽培研究所，黑龙江 哈尔滨150086；
2.黑龙江省农业科学院 作物育种研究所，黑龙江 哈尔滨150086
Author(s):
BI Ying-dong1; LI Wei1; LIN Hong2; XIAO Jia-lei1; LI Wan1; YANG Xue-feng2; LAI Yong-cai1
1.Institute of Crop Tillage and Cultivation,Heilongjiang Academic of Agricultural Sciences,Harbin 150086;
2.Crops Breeding Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang,China
关键词:
大豆\[Glycine max(L.)Merr.\]; 异黄酮; 杂种优势
Keywords:
Soybean〔Glycine max (L.) Merr.〕; Isoflavone; Heterosis
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.02.009
文献标志码:
A
摘要:
选用野生大豆、栽培大豆按异黄酮含量不同（高、中、低）配制杂交组合，对33个组合进行杂种优势分析。结果表明：中亲优势为正向优势的组合为15个，占全部组合的45.5%。具有超高亲优势的组合为12个，其中高异黄硐含量母本组合5个，占全部正向超高亲优势的41.7%，说明高异黄酮亲本杂种优势明显。18个栽培与野生杂交组合中超高亲优势正向优势组合为11个，中亲优势正向优势组合为13个，说明栽培与野生杂交组合的杂种优势明显，但14个栽培×野生杂交组合F2优势降低，表现为自交衰退，因此获得高异黄酮的后代材料仍需进一步选择。
Abstract:
In order to analyze heterosis of isoflavone content in interspecific hybridization,33 hybridized combinations were obtained by 15 accessions of Glycine max and Glycine soja which were different in isoflavone content(high,middle and low).Isoflavone concentrations of F1,F2 as well as their parents were determined by high performance liquid chromatography.Fifteen combinations which accounted for 45.5% of total had positive mid-parent heterosis.Twelve combinations showed positive over-parent heterosis,among which,five combinations had higher isoflavone content in female parent.Among 18 combinations between Glycine max and Glycine soja,11 had positive over-parent heterosis and 13 had mid-parent heterosis.Results suggest heterosis of isoflavone content were obvious when parents with higher isoflavone content were selected or interspecific hybridization were conducted.Heterosis of isoflavone content in 14 interspecific hybridization combinations showed inbreeding depression in F2 generation.Therefore,it is essential to do further selection in following generations for high isoflavone breeding.

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备注/Memo

基金项目：公益性行业（农业）科研专项经费（2000903040）；转基因生物新品种培育重大专项（2011ZX08004-003）；公益性行业（农业）科研专项经费项目（200903007-09）

第一作者简介：毕影东(1974-)，男，博士，现从事野生大豆种质资源创新与利用研究。E-mail:ydbi308@163.com。
通讯作者：来永才(1964-)，男，研究员，博士，现从事野生大豆种质资源创新及利用研究。E-mail:yame0451@163.com。

更新日期/Last Update: 2014-08-15