SHI Feng-yu,ZHU Ying-bo,LONG Ru,et al.Construction and Primary Analysis of a SSH Library of Wild Soybean Induced by SMV[J].Soybean Science,2011,30(06):968-971.[doi:10.11861/j.issn.1000-9841.2011.06.0968]
大豆花叶病毒诱导的野生大豆抑制差减文库构建及初步分析
- Title:
- Construction and Primary Analysis of a SSH Library of Wild Soybean Induced by SMV
- 文章编号:
- 1000-9841（2011）06-0968-04
- 关键词:
- 野生大豆; 大豆花叶病毒病; 抑制差减杂交技术（SSH）; cDNA文库
- Keywords:
- Wild soybean; Soybean Mosaic Virus; Suppression subtractive hybridization(SSH); cDNA library
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以野生大豆Bian0526为试验材料，通过抑制性差减杂交技术（SSH）构建了大豆花叶病毒（SMV）诱导的cDNA差减文库。在文库中共筛选到15 343个阳性克隆，PCR鉴定插入片段大部分集中在500~800 bp之间。利用BLAST在GenBank文库中进行序列相似性比对，获得有功能的EST 200个。对有功能注释的同源分析发现，其功能涉及植物的细胞自身保护和系统获得性抗性等方面，说明这些基因很可能参与到野生大豆抗大豆花叶病毒反应中。
- Abstract:
- To isolate the genes related to wild soybean resistance in Soybean Mosaic Virus(SMV),Suppression Subtractive Hybridization(SSH)was utilized to construct the different expression cDNA library using wild soybean variety Bian0526 inoculated with race 3 of SMV.From this library,a total of 15 343 positive clones were screened,and the length of most of the insert fragments was focused on 500-800 bp.ESTs similarity analysis of these unigenes was performed with the Blastx and Blastn against the non-redundance database in GenBank.The results showed that 200 functional ESTs had been gotten,and they were related to cell self-protection and system acquired resistance,etc.This indicated that these genes might be involved in resistance to SMV of wild soybean.
参考文献/References:
[1]胡吉成，谢淑仪.野生大豆病毒病的初步鉴定[J].植物病理学报,1984,14(2):122-123.(Hu J C,Xie S Y.Preliminary identification of wild soybean(Glycine Soja)virus diseases[J].Acta Phytopathologica Sinica,1984,14(2):122-123.) [2]孙永吉,刘玉芝,胡吉成,等.野生大豆抗花叶病毒病研究[J].大豆科学,1991,10(3):212-216.(Sun Y J,Liu Y Z,Hu J C,et al.A study on resistance of the wild soybeans to soybean mosaic virus(SMV)[J].Soybean Science,1991,10(3):212-216.) [3]来永才,林红,方万程,等.大豆优异资源筛选、评价及利用的研究[J].中国农学通报,2005,21(6):379-382.（Lai Y C,Lin H,Fang W C,et al.Research that the excellent resource of wild soybean screen appraise and utilization in Heilongjiang[J].Chinese Agricultural Science Bulletin,2005,21(6):379-382.） [4]史凤玉,朱英波,龙茹,等.野生大豆抗大豆花叶病毒病评价、聚类及性状间相关分析[J].大豆科学,2010,29(6):976-981.(Shi F Y,Zhu Y B,Long R,et al.Evaluation,cluster analysis for Glycine soja resistant to soybean mosaic virus(SMV)and correlation analysis between characters[J].Soybean Science,2010,29(6):976-981). [5]史凤玉,朱英波,李海潮,等.野生大豆叶片形态结构与抗病毒病关系的研究[J].大豆科学,2008,27(2):52-60.(Shi F Y,Zhu Y B,Li H C,et al.Relationships between SMV resistance and morphological structures in wild soybean[J].Soybean Science,2008,27(2):52-60.) [6]史凤玉,朱英波,龙茹,等.河北东部沿海地区野生大豆病毒病抗性与几种酶活性的关系[J].植物病理学报,2008,38(4):382-387.(Shi F Y,Zhu YB,Long R,et al.Relationships between soybean mosaic virus(SMV)resistance and several enzyme activities from wild soybean in the eastern coastal region of Hebei province[J].Acta Phytopathologica Sinica,2008,38(4):382-387.） [7]李开盛,曹越平.野生大豆抗花叶病毒病生化机制的研究[J].大豆科学,2011,30(2)： 254-258.(Li K S,Cao Y P.Biochemical mechanism of resistance to SMV in wild soybean(Glycine soja.)[J].Soybean Science,2011,30(2):254-258.) [8]Bevan M,Bancroft I,Bent E,et al.Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana[J].Nature,1998,391:485-488. [9]Song W Y,Wang G L,Chen L L,et al.A receptor kinase-like protein encoded by the rice disease resistance gene,Xa21 [J].Science,1995,270(5243):1804-1806. [10]Hofte H,Desprez T,Amselen J,et al.An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana[J].The Plant Journal,1993,4(6):1051-1061. [11]Borch J,Bych K,Roepstorff P,et al.Phosphorylation-independent interaction between 14-3-3 protein and the plant plasma membrane H+-ATPase[J].Biochemical Society Transactions,2002,30(4):411-415. [12]杨郁文,倪万潮,张保龙,等.陆地棉一个丝氨酸/苏氨酸激酶蛋白基因的克隆与表达分析[J].棉花学报,2006,18(3):140-144.(Yang Y W,Ni W C,Zhang B L,et al.Molecular cloning and expression analysis of a serine/threonine protein kinase gene in upland cotton[J].Cotton Science,2006 ,18(3):140-144.) [13]Schuler M A,Daniele W R.Functional genomics of P450s [J].Annual Review of Plant Biology.2003,54:627-629. [14]张萍,潘惠新,黄敏仁,等.杨四瘿螨诱导的美洲黑杨抑制消减文库的构建及分析[J].南京林业大学学报(自然科学版),2009,33(2):5-8.(Zhang P,Pang H X,Huang M Y,et al.Construction and analysis of Populus deltoids SSH cDNA library induced by Tetra lobulifera (Keifer)[J].Journal of Nanjing Forestry University(Natural Science Edition),2009,33(2):5-8.)
相似文献/References:
[1]高越,刘辉,陶波.抗草甘膦野生大豆筛选及其抗性生理机制研究[J].大豆科学,2013,32(01):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
GAO Yue,LIU Hui,TAO Bo.Screening and Physiological Mechanisms of Resistance to Glyphosate in Wild Soybeans(Glycine soja)[J].Soybean Science,2013,32(06):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
[2]王军卫,侯立江,李? 登,等.野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
WANG Jun-wei,HOU Li-jiang,LI Deng,et al.Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(06):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
[3]王军卫,侯立江,李 登,等. 野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.
WANG Jun-wei,HOU Li-jiang,LI Deng,et al. Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(06):596.
[4]王丽燕.硅对野生大豆幼苗耐盐性的影响及其机制研究[J].大豆科学,2013,32(05):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
WANG Li-yan.Effects of Silicon on Salt Tolerance of Glycine soja Seedlings and Its Mechanism[J].Soybean Science,2013,32(06):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
[5]陈丽丽,王明玖,何丽君,等.野生大豆ISSR体系的优化及其在远缘杂交后代鉴定中的利用[J].大豆科学,2013,32(04):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
CHEN Li-li,WANG Ming-jiu,HE Li-jun,et al.Optimization for ISSR Reaction System of Wild Soybean and Its Utilization in Distant Hybrid Identification[J].Soybean Science,2013,32(06):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
[6]郑世英,萧蓓蕾,金桂芳.NaCl胁迫对野生大豆和栽培大豆叶绿素及光合特性的影响[J].大豆科学,2013,32(04):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
ZHENG Shi-ying,XIAO Bei-lei,JIN Gui-fang.Effect of NaCl Stress on Chlorophyll Content and Photosynthetic Characteristics of Glycine soja and Glycine max[J].Soybean Science,2013,32(06):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
[7]韩英鹏,程 章,赵 雪,等.大豆花叶病毒病和疫霉根腐病抗性的SSR标记辅助鉴定[J].大豆科学,2013,32(06):740.[doi:10.11861/j.issn.1000-9841.2013.06.0740]
[8]徐艳平,胡翠美,张文会,等.干旱胁迫对野生大豆幼苗光合作用相关指标的影响[J].大豆科学,2013,32(03):341.[doi:10.11861/j.issn.1000-9841.2013.03.0341]
XU Yan-ping,HU Cui-mei,ZHANG Wen-hui,et al.Effect of Simulated Drought Stress on Photosynthesis Related Indexes at Seedling Stage of Wild Soybeans[J].Soybean Science,2013,32(06):341.[doi:10.11861/j.issn.1000-9841.2013.03.0341]
[9]胡卫静,何丽君,何劲莉,等.NaCl胁迫对野生与栽培大豆杂交后代株系生理指标的影响[J].大豆科学,2013,32(03):349.[doi:10.11861/j.issn.1000-9841.2013.03.0349]
HU Wei-jing,HE Li-jun,HE Jin-li,et al.Effects of NaCl Stress on Physiological Characters of Soybean Hybrids from Glycine max × Glycine soja[J].Soybean Science,2013,32(06):349.[doi:10.11861/j.issn.1000-9841.2013.03.0349]
[10]王 旻,梁 玉,王欣欣,等.即墨野生大豆主要成分及其营养价值分析[J].大豆科学,2013,32(03):355.[doi:10.11861/j.issn.1000-9841.2013.03.0355]
WANG Min,LIANG Yu,WANG Xin-xin,et al.Assessment on Nutritional Compositions and Value of Jimo Wild Soybean[J].Soybean Science,2013,32(06):355.[doi:10.11861/j.issn.1000-9841.2013.03.0355]
[11]史凤玉,朱英波,龙茹,等.野生大豆抗大豆花叶病毒病评价、聚类及性状间相关分析[J].大豆科学,2010,29(06):976.[doi:10.11861/j.issn.1000-9841.2010.06.0976]
SHI Feng-yu,ZHU Ying-bo,LONG Ru,et al.Evaluation, Cluster Analysis for Glycine soja Resistant to Soybean Mosaic Virus (SMV) and Correlation Analysis between Characters[J].Soybean Science,2010,29(06):976.[doi:10.11861/j.issn.1000-9841.2010.06.0976]
备注/Memo
基金项目：国家自然科学基金项目（30940050）；河北省自然科学基金项目(C2006000559,C2009000868)；河北省科技厅项目(06547005D-3，09220103D-11)；河北省教育厅项目（Z2008114）。