SHI Qing,LI Hui-ping,HUANG Da-zhuang,et al.Producing Conditions of Chlamydospore of Fusarium virguliforme sp.nov.in Liquid Culture[J].Soybean Science,2011,30(05):813-817.[doi:10.11861/j.issn.1000-9841.2011.05.0813]
北美大豆猝死综合症病菌厚垣孢子的液体培养条件
- Title:
- Producing Conditions of Chlamydospore of Fusarium virguliforme sp.nov.in Liquid Culture
- 文章编号:
- 1000-9841(2011)05-0813-05
- 关键词:
- 北美大豆猝死综合症病菌; 厚垣孢子; 液体培养条件
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 用液体培养的方法,研究培养基、pH值、温度以及氧气等因素对北美大豆猝死综合症病菌(Fusarium virguforme sp.nov.)菌株F-171厚垣孢子产生的影响。结果表明:菌株F-171产生厚垣孢子的最适液体培养条件为7 500 r·min-1、2 min匀浆断裂菌丝,在250 mL三角瓶装入80 mL大豆茎秆或豆芽汁培养液,在pH 6,25~30℃下,以120 r·min-1振荡培养。最佳液体培养条件下的产孢曲线表明,随培养时间的延长,厚垣孢子产量逐渐增加,10 d后产量最高,达到3.15×105个·mL-1。
- Abstract:
- The effects of culture medium,pH,temperature and oxygen on the chlamydospore production of Fusarium virguliforme sp.nov. strain F-171 were studied by liquid culture method.The results showed that,the best liquid culture conditions for the chlamydospore production of the strain F-171 were,homogenized hyphae at 7500 r·min-1 for 2 min,filled 80 mL liquid culture of soybean stem or sprout juice in the 250 mL flask,and incubated at pH 6,25-30℃ and 120 r·min-1 shaking culitivation environment.Chlamydospore production curve in the best liquid culture conditions showed that the number of chlamydospore increased gradually with prolonging of the incubation time,reached the peak(3.15×105spores·mL-1)10 days after incubation.
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备注/Memo
收稿日期:2011-05-27基金项目:国家质检总局科技计划资助项目(2009IK264)。