HU Shao-xin,JIANG Lian-zhou,XU De-chun,et al.Producing Technology of Antihypertensive Peptide Derived from Soybean Protein Isolated by Enzymatic Method[J].Soybean Science,2011,30(04):643-647.[doi:10.11861/j.issn.1000-9841.2011.04.0643]
大豆分离蛋白生产降血压肽酶解技术研究
- Title:
- Producing Technology of Antihypertensive Peptide Derived from Soybean Protein Isolated by Enzymatic Method
- 文章编号:
- 1000-9841（2011）04-0643-05
- 分类号:
- TS201.1
- 文献标志码:
- A
- 摘要:
- 选用6种蛋白酶水解大豆分离蛋白，根据ACE抑制率高低，最终选择胰蛋白酶为最佳蛋白酶。通过单因素试验和L16（45）正交试验研究底物浓度、水解时间、酶与底物比、温度和pH值对酶解液降血压活性的影响，确定了水解最优条件组合。结果表明：底物浓度5%，水解时间6 h，酶和底物比为0.08，温度为50℃，pH值为8.0时，所得水解液ACE抑制率最高，为76.8%。
- Abstract:
- Trypsinase was selected from six proteinases according to the angiotensin converting enzyme(ACE) inhibitory rate.Single factor experiments and orthogonal design L16(45) were adopted to investigate the effect of substrate concentration,hydrolysis time,E/S,temperature and pH value on ACE inhibitory activity of the product.The optimum trypsinase hydrolysis process for producing ACE inhibitory peptides was under 50℃,with pH 8.0,5% substrate concentration,0.08 E/S for 6 hours.After optimization,the ACE inhibitory activity reached 76.8%.
参考文献/References:
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备注/Memo
基金项目：农业部大豆产业技术体系资助项目（nycytx-004）。