[1]刘琦,夏善勇,张军,等.农杆菌介导植酸酶基因转化大豆的研究[J].大豆科学,2011,30(03):397-400.[doi:10.11861/j.issn.1000-9841.2011.03.0397]
　LIU Qi,XIA Shan-yong,ZHANG Jun,et al.Transformation of Phytase Gene into Soybean via Agrobacterium-mediated Method[J].Soybean Science,2011,30(03):397-400.[doi:10.11861/j.issn.1000-9841.2011.03.0397]
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农杆菌介导植酸酶基因转化大豆的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第30卷 期数: 2011年03期 页码: 397-400 栏目: 出版日期: 2011-06-25
Title:
Transformation of Phytase Gene into Soybean via Agrobacterium-mediated Method
文章编号:
1000-9841(2011)03-0397-04
作者:
刘琦1; 夏善勇1; 张军2; 段忠卫2; 刘鑫磊3; 李希臣1
1.黑龙江省农业科学院 生物技术研究所，黑龙江 哈尔滨 150086；
2.东北农业大学 生命科学学院，黑龙江 哈尔滨 150086；
3.黑龙江省农业科学院 大豆研究所，黑龙江 哈尔滨 150086
Author(s):
LIU Qi1; XIA Shan-yong1; ZHANG Jun2; DUAN Zhong-wei2; LIU Xin-lei3; LI Xi-chen1
1. Biotechnology Research Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086;
2.Life Science College of Northeast Agricultural University，Harbin 150030;
3.Soybean Research Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
关键词:
大豆胚尖; 农杆菌; 植酸酶基因
Keywords:
Embryonic tips of soybean;  Agrobacterium;  Phytase gene
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.03.0397
文献标志码:
A
摘要:
以大豆胚尖为受体，利用农杆菌介导法将来源于泡盛曲霉的植酸酶基因phy转入黑农37和吉林小粒豆中。此法取材方便，操作简单，产生嵌合体的可能性低。在筛选培养基中经草胺膦（1.0 mg·L-1）筛选，获得抗性植株。对获得的草胺磷抗性植株采用除草剂（10%Basta）筛查表型鉴定与目的基因、筛选标记基因PCR分子检测相结合的方法，检测结果直观、可信度高。最终获得5株阳性植株，转化率为0.5%。
Abstract:
The Phy gene cloned from Aspergillus awamori was transformed into soybean varieties Heinong37 and Jilinxiaoli soybean through Agrobacterium-mediated method with soybean embryonic tips as acceptors. The method has advantage of easy operation and lower chimera rate. The regenerated plants were screened by glufosinate-ammonium（1.0 mg·L-1）of medium. The regenerated plantlets resistant to glufosinate-ammonium were detected by combining the PCR analysis of the target gene as well as selective marker gene and the herbicide screening. The result was intuitive and credible. Finally， 5 positive plants were obtained, and the transformation efficiency reached 0.5%.

参考文献/References:

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备注/Memo

基金项目：国家转基因专项资助项目（ZJN04—0402）；黑龙江省农业科学院创新工程资助项目。

第一作者简介：刘琦（1980-），女，硕士，从事作物生物技术研究。 E-mail：liuqi0316@163.com。
通讯作者：李希臣（1968-），男，研究员，主要从事作物生物技术研究。E-mail：lixichen1968@163.com。

更新日期/Last Update: 2014-09-12