ZHANG Yan,LI Xiao-wei,ZHANG Hai-jun,et al.Establishment of an Optimized System about Extraction-Determination of Soybean Isoflavone[J].Soybean Science,2011,30(01):141-143,145.[doi:10.11861/j.issn.1000-9841.2011.01.0141]
大豆异黄酮提取-测定优化体系的建立
- Title:
- Establishment of an Optimized System about Extraction-Determination of Soybean Isoflavone
- 文章编号:
- 1000-9841（2011）01-0141-03
- Keywords:
- Soybean isoflavones; Ultrasonic wave; Three-wave length UV spectrohotometry; Optimized system
- 分类号:
- TS201.1
- 文献标志码:
- A
- 摘要:
- 利用超声波法提取大豆异黄酮，结合三波长紫外分光光度法测定大豆异黄酮含量，对大豆异黄酮提取方法进行优化。结果获得的最优提取条件为：料液比1∶20、乙醇浓度50%、提取时间30 min，提取2次；并用三波长法测定24个大豆品种异黄酮含量，发现品种间异黄酮含量有显著差异，其中吉林32和吉林35含量最高，分别达到了0.292%和0.259%。
- Abstract:
- The method to extract soybean isoflavone by ultrasonic wave was optimized, the content of which was determined by three-wave length UV spectrohotometry, in order to develop a high performance extraction-determination method of isoflavones in soybean. Results showed that the optimized condition were as follows: the ratio of material to solvent was 1∶20,concentration of ethanol was 50%,extraction time was 30 min,with 2 replicates. Twenty-four soybean varieties was mesured, their contents varied significantly. The content of soybean isoflavones was highest in Jilin 32 and Jilin 35,0.292% and 0.259%， respectively.
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备注/Memo
基金项目：转基因生物新品种培育重大专项子课题（2008ZX08004-003）；国家自然科学基金面上项目（30971808）；吉林省科技发展计划重点项目（20080204）。