ZHANG Hong-xia,FENG Rui-hua,GUAN Da-wei,et al.Screening of Superior Soybean Rhizobial Strains and Analyzing of Different Inoculation Methods in Loess Plateau Region of China[J].Soybean Science,2010,29(06):996-1002.[doi:10.11861/j.issn.1000-9841.2010.06.0996]
黄土高原地区优良大豆根瘤菌的筛选与接种方式研究
- Title:
- Screening of Superior Soybean Rhizobial Strains and Analyzing of Different Inoculation Methods in Loess Plateau Region of China
- 文章编号:
- 1000-9841（2010）06-0996-07
- Keywords:
- Screening; Rhizobium; Soybean; Drought-resistant; Superior strain; Inoculation method
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 采用蛭石和土壤盆栽试验，从黄土高原地区17个大豆品种上采集根瘤，对根瘤进行分离纯化、回接验证和遗传多样性分析，根据遗传多样性分析结果、分离宿主和分离地点，选出代表菌株用于筛选与黄土高原地区面积种植最大的大豆品种晋豆25相匹配的优良菌株，并在田间进行了根瘤菌喷施、拌种和种下接种不同接种方式效果比较试验。以瘤数、瘤干重、植株干重、植株全氮量为指标，通过蛭石盆栽试验初筛获得与晋豆25共生匹配效果好的根瘤菌10株；其进一步的耐旱试验和土壤盆栽复筛结果表明：菌株Bradyrhizobium liaoningense 4345和Sinorhizobium fredii 4338在结瘤能力、固氮能力、竞争能力和耐旱性能方面最好，揭示B. liaoningense 4345和S. fredii 4338具有良好应用前景。在田间进行的根瘤菌3种接种方式小区试验中，B. liaoningense 4345喷施处理在大豆植株干重、植株全氮量、占瘤率和产量等方面均显著高于另2个处理和对照，S. fredii 4338拌种和喷施较好，表明黄土高原地区大豆根瘤菌的适宜接种方式是喷施和拌种。
- Abstract:
- Ten superior rhizobia strains matched well with the soybean (Glycine max) cultivar Jindou 25 were screened from 20 rhizobia varieties that isolated from Loess Plateau region according to nodule numbers, nodule dry weights, plant dry weights and total N contents by vermiculite and soil pot experiments. Subsequently, three inoculation methods were adopted in field experiments and two strains of Bradyrhizobium liaoningense 4345 and Sinorhizobium fredii 4338 were selected by their performance of nitrogen fixing and competitive nodulation ability from the 10 strains in drought resistant and soil pot experiments. Spraying showed better effects in plant dry weight, nodulation competitiveness, seed yield and total N content than the other two treatments for B. liaoningense 4345. Moreover, seed dressing showed the best effects in plant dry weight, nodulation competitiveness, seed yield and total N content for S. fredii 4338 in the field experiment. The results indicated that the inoculation methods of spraying and seed dressing were Suitable for rhizobium application in Loess Plateau region.
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备注/Memo
基金项目：现代农业产业技术体系建设专项资助项目(nycytx-0O4)；中央级公益性科研院所科研业务费专项资助项目（2010-34）。