LUO Qiu-lan,ZHOU Shi,ZHAO Lin,et al.Study on Hypocotyl-based Agrobacterium-mediated Transformation of Soybean[J].Soybean Science,2010,29(06):925-929.[doi:10.11861/j.issn.1000-9841.2010.06.0925]
农杆菌介导的下胚轴法转化大豆研究
- Title:
- Study on Hypocotyl-based Agrobacterium-mediated Transformation of Soybean
- 文章编号:
- 1000-9841（2010）06-0925-05
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以下胚轴为外植体，采用农杆菌介导方法成功将大豆的蓝光受体启动子pGmPLP1导入大豆基因组中，并且该基因在后代中得到稳定遗传表达。比较了栽培品种黑农44用子叶节法和下胚轴法转化的区别，发现用子叶节法不能获得高效转基因植株的品种可以顺利通过下胚轴法进行转化。
- Abstract:
- The study successfully transformed the soybean blue light receptor gene promoter pGmPLP1 into soybean genome by hypocotyl-based Agrobacterium-mediated transformation method, and the gene was steady expressed in progenies. Also the comparison between cultivar Heinong 44 transformed by cotyledon nodes and hypocotyls was conducted. The results showed that the cultivars that cannot efficiently transformed by cotyledon nodes method could successfully transformed by hypocotyl method.
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备注/Memo
基金项目：国家自然科学基金面上项目(30971810)。