CHENG Chun-ming,YANG Cun-yi,MA Qi-bin,et al.Diversity Analysis of GmPAP1 of Wild Soybean in South China[J].Soybean Science,2010,29(06):920-924.[doi:10.11861/j.issn.1000-9841.2010.06.0920]
华南野生大豆GmPAP1基因多样性分析
- Title:
- Diversity Analysis of GmPAP1 of Wild Soybean in South China
- 文章编号:
- 1000-9841(2010)06-0920-05
- Keywords:
- Wild soybean; GmPAP1; Diversity
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- GmPAP1属低磷诱导基因,通过同源克隆测序,分析68份来源于华南亚热带地区江西、湖南、福建、广西等地野生大豆GmPAP1基因的多样性。与原基因序列GmPAP1(AF236108)比较, 68份种质资源共有22个SNPs和6个Indels,22个SNPs中有13处发生转换,9处发生颠换,转换所占比例接近2/3,有14个发生了非同义突变,表明该基因在不同地区的大豆中具有较高的多样性。
- Abstract:
- GmPAP1 is a gene induced by low-phosphorus condition, the sequence polymorphisms of GmPAP1 in comparison with the control gene sequence (AF236108)from Genebank were analyzed by cloning in 68 wild soybean genotypes mainly from the subtropical zone: Jiangxi, Hunan, Fujian province and Guangxi Autonomous Region in South China. The results showed that, 22 SNPs and 6 Indels were found. Among these polymorphic sites, 13 transition, 9 transversion and 14 non-synonymous mutations were detected, indicating the abundance of gene diversity among these genotypes.
参考文献/References:
[1]Kim M Y, Ha B K, Jun T H, et al. Single nucleotide polymorphism discovery and Linkage mapping of lipoxygenase-2gene(Lx2)in soybean[J]. Euphytiea, 2004, 135: 169-177. [2]Batley J, Barker G, Sullivan H O, et al. Mining for single nueleotide polymorphisms and insertions/deletions in maize expressed sequence tag data [J]. Plant Physiology, 2003, 132: 84-91. [3]李英慧. 中国大豆地方品种遗传多样性分析及大豆抗胞囊线虫SNAP标记发掘[D]. 北京:中国农业科学院,2005:56-58.(Li Y H. Genetic structure and diversity for chinese soybean (Glycine max L.) landrances in China and SNAPs marker development at rhg1?locus underlying resistance to Soybean Cyst Nematode[D]. Beijing: Chinese Academy of Agricultural Sciences, 2005: 56-58.) [4]严小龙,廖红,年海, 等. 根系生物学:原理与应用[M]. 北京:科学出版社,2007: 138-140.(Yan X L, Liao H, Nian H, et al. Root biology: Principles and Applications[M]. Beijing:Science Press, 2007: 138-140.) [5]李东屏,王道文. 拟南芥紫色酸性磷酸酶基因(AtPAP)对磷饥饿的响应[J]. 生命科学研究,2003,7(1):65-69. (Li D P, Wang D W. Responses of putative purple acid phosphatase genes in Arabidopsis thaliana (AtPAP) to phosphorus starvation[J] .Life Science Research, 2003, 7(1):65-69.) [6] Schenk G, Guddat L W, Ge Y, et al. Identification of mammalian-like purple acid phosphatases in a wide range of plants[J]. Gene, 2000, 250: 117-125. [7]Liao H, Wonga F L, Phang T H, et al.?GmPAP3,?a novel purple acid phosphatase-like gene in soybean induced by NaCl stress but not phosphorus deficiency [J]. Gene, 2003, 318: 103-111. [8]邹洪锋. 野生、半野生和栽培大豆的单核苷酸多态性[D]. 南昌:南昌大学,2005. (Zhou H F. Single nucleotide polymorphisms in G.soja, G.gracilis?and G.max[D]. Nanchang: Nanchang University, 2005.) [9]Lander E S. The new genomics: global views of biology [J]. Science, 1996, 274: 536-539. [10]邹喻苹,葛颂. 新一代分子标记—SNPs及其应用生物多样性[J]. 生物多样性,2003,11(5):370-382. (Zhou Y P, Ge S. A novel molecular marker—SNPs and its application [J]. Biodiversity Science, 2003,11(5):370-382.) [11]Halushka M K, Fan J B, Bentley K, et al. Patterns of single nucleotide polymorphisms in candidate genes for blood-pressure homeostasis[J]. Nature Genetics, 1999, 22(3):239-247. [12]Tenaillon M I, Sawkins M C, Long A D, et al. Patterns of DNA sequence polymorphisms along chromosome 1 of maize(Zea mays L)[J]. Proceedings of the National Academy of Sciences, 2001, 98(16):9161-9166. [13]徐豹,徐航,庄炳昌,等. 中国野生大豆(G. soja) 籽粒性状的遗传多样性及其地理分布[J]. 作物学报, 1995,21(6):733-739 .( Xu B, Xu H, Zhuang B C, et al. Polymorphism and geographical distribution of seed characters of wild soybean (G.soja)?in China[J]. Acta Agronomica Sinica,1995, 21(6):733-739.) [14]董英山,庄炳昌,赵丽梅,等.中国野生大豆遗传多样性中心[J]. 作物学报,2000,26(5):521-527.(Dong Y S, Zhuang B C, Zhao L M,et al. The Genetic diversity centers of annual wild soybean in China[J]. Acta Agronomica Sinica, 2000,26(5):521-527.) [15]丁艳来,赵团结,盖钧镒. 中国野生大豆的遗传多样性和生态特异性分析[J]. 生物多样性,2008,16(2):133-142. (Ding Y L,Zhao T J,Gai J Y . Genetic diversity and ecological differentiation of chinese annual wild soybean(G.soja) [J]. Biodiversity Science 2008,16(2):133-142.) [16]Wang K J, Li X H, Li F S. Phenotypic diversity of the big seed type subcollection of wild soybean (Glycine soja?Sieb. et Zucc.) in China[J]. Genetic Resources and Crop Evolution, 2008, 55:1335-1346.
相似文献/References:
[1]高越,刘辉,陶波.抗草甘膦野生大豆筛选及其抗性生理机制研究[J].大豆科学,2013,32(01):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
GAO Yue,LIU Hui,TAO Bo.Screening and Physiological Mechanisms of Resistance to Glyphosate in Wild Soybeans(Glycine soja)[J].Soybean Science,2013,32(06):76.[doi:10.3969/j.issn.1000-9841.2013.01.018]
[2]王军卫,侯立江,李? 登,等.野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
WANG Jun-wei,HOU Li-jiang,LI Deng,et al.Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(06):596.[doi:10.11861/j.issn.1000-9841.2013.05.0596]
[3]王军卫,侯立江,李 登,等. 野生大豆紫色酸性磷酸酶PAP1基因的克隆及分析[J].大豆科学,2013,32(05):596.
WANG Jun-wei,HOU Li-jiang,LI Deng,et al. Cloning and Sequence Analysis of Purple Acid Phosphotase PAP1 Gene in Wild Soybean[J].Soybean Science,2013,32(06):596.
[4]王丽燕.硅对野生大豆幼苗耐盐性的影响及其机制研究[J].大豆科学,2013,32(05):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
WANG Li-yan.Effects of Silicon on Salt Tolerance of Glycine soja Seedlings and Its Mechanism[J].Soybean Science,2013,32(06):659.[doi:10.11861/j.issn.1000-9841.2013.05.0659]
[5]陈丽丽,王明玖,何丽君,等.野生大豆ISSR体系的优化及其在远缘杂交后代鉴定中的利用[J].大豆科学,2013,32(04):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
CHEN Li-li,WANG Ming-jiu,HE Li-jun,et al.Optimization for ISSR Reaction System of Wild Soybean and Its Utilization in Distant Hybrid Identification[J].Soybean Science,2013,32(06):459.[doi:10.11861/j.issn.1000-9841.2013.04.0459]
[6]郑世英,萧蓓蕾,金桂芳.NaCl胁迫对野生大豆和栽培大豆叶绿素及光合特性的影响[J].大豆科学,2013,32(04):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
ZHENG Shi-ying,XIAO Bei-lei,JIN Gui-fang.Effect of NaCl Stress on Chlorophyll Content and Photosynthetic Characteristics of Glycine soja and Glycine max[J].Soybean Science,2013,32(06):486.[doi:10.11861/j.issn.1000-9841.2013.04.0486]
[7]徐艳平,胡翠美,张文会,等.干旱胁迫对野生大豆幼苗光合作用相关指标的影响[J].大豆科学,2013,32(03):341.[doi:10.11861/j.issn.1000-9841.2013.03.0341]
XU Yan-ping,HU Cui-mei,ZHANG Wen-hui,et al.Effect of Simulated Drought Stress on Photosynthesis Related Indexes at Seedling Stage of Wild Soybeans[J].Soybean Science,2013,32(06):341.[doi:10.11861/j.issn.1000-9841.2013.03.0341]
[8]胡卫静,何丽君,何劲莉,等.NaCl胁迫对野生与栽培大豆杂交后代株系生理指标的影响[J].大豆科学,2013,32(03):349.[doi:10.11861/j.issn.1000-9841.2013.03.0349]
HU Wei-jing,HE Li-jun,HE Jin-li,et al.Effects of NaCl Stress on Physiological Characters of Soybean Hybrids from Glycine max × Glycine soja[J].Soybean Science,2013,32(06):349.[doi:10.11861/j.issn.1000-9841.2013.03.0349]
[9]王 旻,梁 玉,王欣欣,等.即墨野生大豆主要成分及其营养价值分析[J].大豆科学,2013,32(03):355.[doi:10.11861/j.issn.1000-9841.2013.03.0355]
WANG Min,LIANG Yu,WANG Xin-xin,et al.Assessment on Nutritional Compositions and Value of Jimo Wild Soybean[J].Soybean Science,2013,32(06):355.[doi:10.11861/j.issn.1000-9841.2013.03.0355]
[10]程鹏,徐鹏飞,范素杰,等.野生大豆接种大豆疫霉根腐病菌后过氧化物酶(POD)活性变化[J].大豆科学,2013,32(02):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
CHENG Peng,XU Peng-fei,FAN Su-jie,et al.Response of POD Activity in Glycine soja ?Inoculated by Phytophthora sojae[J].Soybean Science,2013,32(06):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
备注/Memo
基金项目:国家自然科学基金资助项目(30771364)。