MA Chao,HU Chao,LIU Ya-guang.Optimization of Imazethapyr Degradation Bacteria Z Fermentation Medi[J].Soybean Science,2010,29(01):84-87.[doi:10.11861/j.issn.1000-9841.2010.01.0084]
咪唑乙烟酸降解菌Z发酵培养基的优化
- Title:
- Optimization of Imazethapyr Degradation Bacteria Z Fermentation Medi
- 文章编号:
- 1000-9841(2010)01-0084-04
- 分类号:
- Q815
- 文献标志码:
- A
- 摘要:
- 采用摇瓶培养法对咪唑乙烟酸降解菌株Z的发酵培养基配方进行了研究;并通过单因子试验确定咪唑乙烟酸降解菌株Z的碳源、氮源及无机盐,同时应用正交试验设计对发酵培养基组分进行优化。结果表明:优化后确定的发酵培养基的配方为:麦麸皮1.5%,米糠 2%,豆粕 1.5%,酵母粉1.5%,NaCl 0.8%,KH2PO40.01%,MnCl2 0.03%。该研究结果为大规模发酵生产奠定了基础。
- Abstract:
- The paper studied on the fermentation medium of imazethapyr degradation bacteria Z by shake-flask experiment.The carbon sources,nitrogen sources and inorganic salt of imazethapyr degradation bacteria Z were determined by single ingredient experiment.The factors of fermentation medium were optimized by orthogonal design.The optimal fermentation medium was composed of wheat bran 1.5%,rice bran 2%,soybean meal 1.5%,yeast 1.5%,NaCl 0.8%,KH2PO4 0.01%,MnCl2 0.03%,and the optimized results is suitablefor large scale fermentation production.
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备注/Memo
基金项目:黑龙江省自然科学基金资助项目(C2007-20);大豆生物学省部共建教育部重点实验室开放基金资助项目(SB08B03)。