ZUO Yu-hu,YU Hong-mei,SONG Yu-hua.Induced Mutation Effect of Phytophthora sojae by Ultraviolet Irradiating[J].Soybean Science,2008,27(03):490-6495.[doi:10.11861/j.issn.1000-9841.2008.03.0490]
紫外线对大豆疫霉菌的诱导变异作用
- Title:
- Induced Mutation Effect of Phytophthora sojae by Ultraviolet Irradiating
- 文章编号:
- 1000-9841(2008)03-0490-06
- Keywords:
- Phytophthora sojae; Mutation; Ultraviolet irradiating
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 为明确紫外线对大豆疫霉菌的诱导突变作用,研究了紫外线诱变的突变菌株的菌落形态、菌丝生长速率、菌丝形态、卵孢子形态、产孢能力、同宗配合能力、致病力和抗药性的变异情况。结果表明,大豆疫霉菌突变菌株菌落形态、菌丝生长速率、菌丝和卵孢子形态以及同宗配合能力都发生显著变异,游动孢子产孢量和致病性明显降低。虽未发现对甲霜灵产生抗性的突变菌株,但出现了对甲霜灵敏感性增强的突变菌株。说明紫外线辐射可导致大豆疫霉菌控制生物学性状和致病性的基因发生变异,紫外线可能是引致大豆疫霉菌变异的因素之一。
- Abstract:
- To know the induced mutation effect of Phytophthora sojae by ultraviolet irradiating,the inheritance and variation of biological characteristics,pathogenicity and resistance to metalaxyl of P.sojae-mutant strains were studied.These biological characteristics include the colony morphology,mycelia growth rate,mycelia morphology,oospore morphology,sporulation,homothallism.The results indicated that the colony morphology,mycelia growth rate,mycelia morphology,oospore morphology and homothallism had obvious variation.The sporulation and pathogenicity of P.sojae-mutant strains were reduced.The sensitivity to metalaxyl of some-P.sojae-mutant strains were raised though there were not resistance to metalaxyl P.sojae-mutant strains.The genes which control the biological characteristics and pathogenicity of P.sojae-can be mutated by ultraviolet irradiating.
参考文献/References:
[1]Schmitthenner A F.Phytophthora Rot[M]//Sinclair J B,Backman P A.Compendium of soybean diseases(Third Edition).Minnesota: The American Phytopathological Society Press,1989:35-38.
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备注/Memo
基金项目:黑龙江省普通高等学校骨干教师创新能力资助计划(1055G027)。