[1]Shuxian-Li,Anatoliy Lygin,Olga Zernova,等.接种茄病镰刀菌（Fusarium solani f. sp. glycines）对不同基因型大豆植株和须根的影响[J].大豆科学,2008,27(02):275-282.[doi:10.11861/j.issn.1000-9841.2008.02.0275]
　Shuxian-Li,Anatoliy Lygin,Olga Zernova,et al.Genotype Response of Soybean (Glycine max) Whole Plants and Hairy Roots to Fusarium solani f.sp.glycines Infection[J].Soybean Science,2008,27(02):275-282.[doi:10.11861/j.issn.1000-9841.2008.02.0275]
点击复制

接种茄病镰刀菌（Fusarium solani f. sp. glycines）对不同基因型大豆植株和须根的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年02期 页码: 275-282 栏目: 出版日期: 2008-04-30
Title:
Genotype Response of Soybean (Glycine max) Whole Plants and Hairy Roots to Fusarium solani f.sp.glycines Infection
文章编号:
1000-9841(2008)02-0275-08
作者:
Shuxian-Li1;  Anatoliy Lygin2;  Olga Zernova2;  Vera Lozovaya2;  Glen L. Hartman3; Jack M. Widholm4
1United States Department of Agriculture-Agricultural Research Services (USDA-ARS), Crop Genetics & Production Research Unit, Stoneville, MS 38776; 2 Department of Crop Sciences, University of Illinois; 3 USDA-ARS and Department of Crop Sciences, University of Illinois; 4 Department of Crop Sciences, University of Illinois, 1101 W. Peabody Dr. Urbana, IL 61801, USA
Author(s):
Shuxian-Li1; Anatoliy Lygin2; Olga Zernova2; Vera Lozovaya2; Glen L.Hartman3; Jack M.Widholm4
1 United States Department of Agriculture-Agricultural Research Services (USDA-ARS)，Crop Genetics & Production Research Unit，Stoneville，MS 38776；2Department of Crop Sciences，University of Illinois;3 USDA-ARS and Department of Crop Sciences，University of Illinois；4 Department of Crop Sciences，University of Illinois，1101 W.Peabody Dr.Urbana，IL 61801，USA
关键词:
细菌; 根毛; 大豆; 猝死综合症
Keywords:
Fungal pathogen; Hairy roots; Soybean; Sudden death syndrome
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.02.0275
文献标志码:
A
摘要:
茄病镰刀菌(Fusarium solani f. sp. glycine)是一种土生细菌，通过侵染大豆根系引发猝死综合症。利用温室盆栽试验和须根培养试验研究了接种茄孢镰刀菌对13个不同基因型大豆的影响。结果表明：接种后所有盆栽大豆主根都有明显深褐色的侵染病斑；移植后21 d 测定了盆栽植株叶部发病程度，Peking 表现最为严重，然后依次为Spencer,Ripley,P3981,Williams 82,Essex,Forrest,Iroquois,PI 520733,Hartwig,PI 567650B,Jack,和PI 567374。叶部发病程度与冠高(r=-0.422P=0.0018)、冠重(r=-0.857,P< 0.0001)和根重(r=-0.732,P<0.0001)呈显著负相关。主根病斑长度与叶部发病程度没有相关性，表明大豆对病原菌的抗性不能仅通过根系得到充分控制。对培养的大豆须根接种茄病镰刀菌菌丝体10 d 后，不同基因型大豆的菌落直径的变化范围为17～40 mm,差异显著 (P=0.05)，其中Spencer 和 Peking须根上的菌落直径显著(P=0.05)大于PI 567374 和 PI 520733。对Spencer 和PI 567374的须根接种10 μL茄病镰刀菌常量成分悬浮液，10 d 后菌落直径分别为50 和38 mm，差异显著(P=0.05)。通常，不同基因型大豆间茄病镰刀菌在培养须根上的生长与整株的症状间有一定的相关性，但不总是这样，这是因为即使根系对毒素产生抗性来减少叶部病害症状，但并不是所有的大豆都表现出明显的根系抗性。
Abstract:
Fusarium solani f.sp.glycines，a soilborne fungus，infects soybean roots and causes sudden death syndrome.The response of 13 soybean genotypes to F.solani f.sp.glycines infection was tested with potted greenhouse grown plants and with cultured hairy roots.The taproots of all genotypes grown in the greenhouse had dark brown lesions following inoculation.Foliar disease severity for greenhouse grown plants measured 21 days after planting was greatest for Peking，followed by Spencer，Ripley，P3981，Williams 82，Essex，Forrest，Iroquois，PI 520733，Hartwig，PI 567650B，Jack，and PI 567374.There were significant negative correlations between foliar disease severity and shoot length(r=-0.422，P=0.0018)，shoot weight (r=-0.857，P<0.0001)，root weight (r=-0.732，P<0.0001)，and total plant dry weights(r=-0.855，P<0.0001).The taproot lesion lengthwas not correlated with foliar disease severity indicating that soybean resistance may not be fully controlled at the root level.When cultured hairy roots were inoculated with F.solani f.sp.glycines mycelial plugs，the colony diameters after 10 days were significantly (P=0.05)different among soybean genotypes ranging from 17 to 40 mm.Fungal colony diameters on hairy roots of Spencer and Peking were greater(P=0.05)than on PI 567374 and PI 520733.In another experiment，following inoculation of Spencer and PI 567374 hairy roots with 10 μL of F.solani f.sp.glycines macroconidial suspensions，10-day-colony diameters were 50 and 38 mm，respectively (P=0.05).While there was generally a correlation between the growth of F.solani f.sp.glycines on the cultured hairy roots and the whole plant symptoms of the different genotypes，this was not always the case.The exceptions may be due to the fact that none of the genotypes showed clear root resistance even though they may show toxin resistance that would result in fewer foliar symptoms.

参考文献/References:

[1]Roy K W.Fusarium solani on soybean roots: Nomenclature of the causal agent of sudden death syndrome and identity and relevance of F.solani form B[J].Plant Disease，1997,81: 259-266.

[2]Roy K W，Lawrence G W，Hodges H H，et al.Sudden death syndrome of soybean Fusarium solani as incitant and relation of Heterodera glycines to disease severity[J].Phytopathology，1989,79: 191-197.
[3]Rupe J C.Frequency and pathogenicity of Fusarium solanirecovered from soybeans with sudden death syndrome[J].Plant Disease，1989,73: 581-584.
[4]Rupe J C，Hartman G L.Sudden death syndrome［M］∥Compendium of Soybean Diseases，4th?ed.Hartman G L，Sinclair J B，Rupe J C.American Phytopathological Society，St.Paul，MN.1999:37-39.
[5]Wrather J A，Koenning S R，Anderson T R.Effect of diseases on soybean yields in the United States and Ontario (1999-2002)[J].Plant Health Progress，2003,doi:10.1094/PHP-2003-0325-01-RV.
[6]Hartman G L，Huang Y H，Nelson R L，et al.Germplasm evaluation of Glycine max for resistance to Fusarium solani，the causal organism of sudden death syndrome[J].Plant Disease，1997，81: 515-518.
[7]Mueller D S，Hartman G L，Nelson R L，et al.Evaluation of Glycine max germplasm for resistance to Fusarium solanif.sp.glycines[J].Plant Disease，2002,86: 741-746.
[8]Huang Y H，Hartman G L.Reaction of selected soybean genotypes to isolates of Fusarium solani f.sp.glycines and their culture filtrates[J].Plant Disease,1998,82: 999-293.
[9]Mueller D S，Li S，Hartman G L，et al.Use of aeroponic chambers and grafting to study partial resistance to Fusarium solani f.sp.glycines in soybean[J].Plant Disease，2002，86: 1223-1226
[10]Luo Y，Myers O，Lightfoot D A,et al.Root colonization of soybean cultivars in the field by Fusarium solanif.sp.glycines［J］.Plant Disease,1999，83: 1155-1159.
[11]Njiti V N，Johnson J E，Torto T A，et al.Inoculum rate influences selection for field resistance to soybean sudden death syndrome in the greenhouse[J].Crop Science，2001，41: 1726-1731.
[12]Hillstrand D S，Auld D L.Comparative evaluation of four techniques for screening winter peas(Pisum sativum ssp.arvense)for resistance to Phoma medicaginisvar.pinodella[J].Crop Science，1982，22: 282-287.
[13]Bristow P R，Wyllie T D.Reaction of soybean cultivars to Macrophomina phaseolina as seedlings in the growth chamber and field[J].Transactions of the Missouri Academy of Science，1984,18: 5-10.
[14]Cho J H，Rupe J C，Cummings M S，et al.Isolation and identification of Fusarium solani f.sp.glycines from soil on modified Nash and Snyder’s medium[J].Plant Disease，2001,85: 256-260.
[15]Tsror L，Hazanovsky M.Effect of coinoculation by Verticillium dahliae and Colletotrichum coccodes on disease symptoms and fungal colonization in four potato cultivars[J].Plant Pathology (Oxford)，2001，50: 483-488.
[16]Gray L E，Achenbach L A.Severity of foliar symptoms and root and crown rot of soybean inoculated with various isolates and inoculum rates of Fusarium solani[J].Plant Disease，1996，80: 1197-1199.
[17]Wagner R E，Wilkinson H T.An aeroponics system for investigating disease development on soybean taproots infected with Phytophthora sojae[J].Plant Disease，1992,76: 610-614.
[18]Du Toit L J，Kirby H W，Pedersen W L.Evaluation of an aeroponics system to screen maize genotypes for resistance to Fusarium graminearum seedling blight[J].Plant Disease，1997,81: 175-179.
[19]Morgan J A，Shanks J V.Inhibitor studies of tabersonine metabolism in Catharanthus roseus hairy roots[J].Phytochemistry (Oxford)，1999,51: 61-68.
[20]Kifle S，Shao M，Jung C，et al.An improved transformation protocol for studying gene expression in hairy roots of sugar beet (Beta vulgaris L.)[J].Plant Cell Reports，1999,18: 514-519.
[21]Cho H J，Farrand S K，Noel G R，et al.High-efficiency induction of soybean hairy roots and propagation of the soybean cyst nematode[J].Planta，2000,210: 195-204.
[22]Savka M A，Ravillion B，Noel G R，et al.Induction of hairy roots on cultivated soybean genotypes and their use to propagate the soybean cyst nematode[J].Phytopathology，1990，80: 503-508.
[23]Lozovaya V V，Lygin A V，Zernova O V，et al.Isoflavonoid accumulation in soybean hairy roots upon treatment with Fusarium solani[J].Plant Physiology and Biochemistry，2004,42: 671-679.
[24]Lozovaya V V，Lygin A V，Zernova O V，et al.Modification of phenolic metabolism in soybean hairy roots through down regulation of chalcone synthase or isoflavone synthase[J].Planta，2007，225: 665-679.
[25]Mugnier J，Mosse B.Vesicular-arbuscular mycorrhizal infection in transformed root-inducing T DNA roots grown axenically[J].Phytopathology,1987,77: 1045-1050.
[26]Stephens P A，Nickell C D，Lim S M.Sudden death syndrome development in soybean cultivars differing in resistance to Fusarium solani[J].Crop Science，1993,33: 63-66.
[27]Li S，Lygin A V，Zernova O V，et al.Response of hairy roots of different soybean genotypes to Fusarium solani f.sp.glycines[J].Phytopathology，2002,92: S47.
[28]Murashige T，Skoog F.A revised medium for rapid growth and bioassays with tobacco tissue cultures[J].Physiologia Plantarum，1962,5: 473-498.
[29]Gamborg O L，Miller R A，Ojima K.Nutrient requirements of suspension cultures of soybean root cells［J］.Experimental Cell Research[J].1968，50: 151-158.
[30]Huang Y H，Hartman G L.A semi-selective medium for detecting Fusarium solani，the causal organism of soybean sudden death syndrome[J].Phytopathology，1996，86: S12.
[31]Li S，Hartman G L,Widholm J M.Viability staining of soybean suspension-cultured cells and a seedling stem cutting assay to evaluate phytotoxicity of Fusarium solani f.sp.glycines culture filtrates[J].Plant Cell Reports，1999,18: 375-380.

相似文献/References:

[1]黄姗姗,段玉玺,陈立杰,等.诱导大豆抗逆细菌的筛选及分子鉴定[J].大豆科学,2011,30(02):205.[doi:10.11861/j.issn.1000-9841.2011.02.0205]
　HUANG Shan-shan,DUAN Yu-xi,CHEN Li-jie,et al.Screening and Molecular Identification of Bacteria Induced Anti-adversity Effect in Soybean[J].Soybean Science,2011,30(02):205.[doi:10.11861/j.issn.1000-9841.2011.02.0205]
[2]罗在全,段玉玺,王媛媛,等.细菌SnebYK发酵液诱导大豆抗阿特拉津的研究[J].大豆科学,2010,29(02):284.[doi:10.11861/j.issn.1000-9841.2010.02.0284]
　LUO Zai-quan,DUAN Yu-xi,WANG Yuan-yuan,et al.Fermentation Broth of Bacteria SnebYK Induced Anti-atrazine Effect in Soybean[J].Soybean Science,2010,29(02):284.[doi:10.11861/j.issn.1000-9841.2010.02.0284]
[3]曲晓华,戴艳婷,赵晓燕.基于rDNA测序的连作大豆土壤微生物群落变化规律研究[J].大豆科学,2024,43(05):632.[doi:10.11861/j.issn.10009841.2024.05.0632]

备注/Memo

Shuxian-Li, Ph. D. Research Plant Pathologist.E-mail: shuxian.li@ars.usda.gov; Phone: 1-(662) 686-3061; Fax: 1-(662) 686-5218.

更新日期/Last Update: 2014-10-10