[1]文瑞芝,李水芳,曾栋.染料木素和大豆素的结构分析[J].大豆科学,2008,27(01):128-133.[doi:10.11861/j.issn.1000-9841.2008.01.0128]
　WEN Rui-zhi,LI Shui-fang,and ZENG Dong.Structure Analysis of Genistein and Daidzein[J].Soybean Science,2008,27(01):128-133.[doi:10.11861/j.issn.1000-9841.2008.01.0128]
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染料木素和大豆素的结构分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年01期 页码: 128-133 栏目: 出版日期: 2008-02-25
Title:
Structure Analysis of Genistein and Daidzein
作者:
文瑞芝1; 李水芳1; 曾栋2
1 中南林业科技大学理学院，湖南长沙 410004；
2 湖南省疾病预防控制中心，湖南长沙 410005
Author(s):
WEN Rui-zhi1; LI Shui-fang1; and ZENG Dong2
1 Central South University of Forestry & Technology，Changsha 410004，Hunan；
2 Hunan Center of Disease Control and Prevention，Changsha 410005，Hunan，China
关键词:
染料木素; 大豆素; 质谱; 红外光谱; 核磁共振谱; 二维核磁共振谱; 结构分析
Keywords:
Genistein; Daidzein; Mass spectrum; Infrared spectrum; Nuclear magnetic resonance spectrum; Two-dimension NMR spectrum; Structure analysis
DOI:
10.11861/j.issn.1000-9841.2008.01.0128
文献标志码:
A
摘要:
染料木素和大豆素是大豆异黄酮苷元的两种主要成分，具有多种生物活性，关于其混合物的同时结构分析，鲜有报道。实验为确保混合物结构解析的正确性，利用质谱技术得出的物质分子离子峰及相关特征碎片峰、红外光谱技术所得的特征官能团信息、核磁共振（氢谱、碳谱及二维核磁共振谱等）技术分析出的碳氢等元素的结构分布情况，从化学式量、主要官能团及具体分子结构等方面成功地对染料木素和大豆素的混合物进行了分子结构的同时佐证解析。这避免了实验必须对混合物进行分离收集，再对各物质单独结构分析所带来的繁琐技术操作，使得方法简便，快捷省时。同时，实验所得方法为其他混合物结构解析提供技术参考。
Abstract:
Genistein and daidzain are two primary soybean isoflavones，which have important biological activities.There are few reports about their structure analysis at the same time.The molecular structures of genistein and daidzain were analyzed at the same time by mass spectrum，infrared spectrum，1H-nuclear magnetic resonance(NMR) spectrum，13C-NMR spectrum and Two-dimension-NMR spectrum，which offers a useful method for the fingerprint analysis of mixture.To make sure the truth of mixture structure analysis，the article analyzed the molecular structures of genistein and daidzain from the aspect of molecular weight，primary function group and material structure.The method makes sure of the correctness of mixtures’structures and avoides separating mixtures before analyzed.The method is proved to be easy and fast.

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备注/Memo

基金项目：中南林业科技大学人才引进科研启动经费(科研101-0648项)

作者简介：文瑞芝（1978-），女，讲师，硕士，主要从事药用植物功效成分的分离分析及结构解析。Tel：013787121542；E-mail：csuft_wrz@126.com

更新日期/Last Update: 2014-10-09