[1]杨向东,郭东全,包绍君,等.双价抗虫转基因大豆抗苜蓿夜蛾分析[J].大豆科学,2007,26(06):969-971.[doi:10.3969/j.issn.1000-9841.2007.06.033]
　YANG Xiang-dong,GUO Dong-quan,BAO Shao-jun,et al.RESISTANCE ANALYSIS OF THE BINARY INSECT-RESISTANT TRANSGENIC SOYBEAN TO HELIOTHIS VIRIPLACA[J].Soybean Science,2007,26(06):969-971.[doi:10.3969/j.issn.1000-9841.2007.06.033]
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双价抗虫转基因大豆抗苜蓿夜蛾分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年06期 页码: 969-971 栏目: 出版日期: 2007-12-25
Title:
RESISTANCE ANALYSIS OF THE BINARY INSECT-RESISTANT TRANSGENIC SOYBEAN TO HELIOTHIS VIRIPLACA
文章编号:
1000-9841(2007)06-0969-03
作者:
杨向东; 郭东全; 包绍君; 赵桂兰; 康岭生; 钱雪燕; 尹爱萍; 邢国杰
吉林省农业科学院生物技术研究中心，长春 130124
Author(s):
YANG Xiang-dong; GUO Dong-quan; BAO Shao-jun; ZHAO Gui-lan; KANG Ling-sheng; QIAN Xue-yan; YIN Ai-ping; XING Guo-jie
Biotechnology Research Center,Jilin Academy of Agriculture Sciences,Changchun 130124
关键词:
苜蓿夜蛾; 转基因大豆; 抗虫性
Keywords:
Heliothis viriplaca; Transgenic soybean; Insect resistance
分类号:
S565.103.4
DOI:
10.3969/j.issn.1000-9841.2007.06.033
文献标志码:
A
摘要:
苜蓿夜蛾是影响我国大豆生产的重要食叶性害虫之一。在实验室和网室人工接虫条件下，对已获得的遗传稳定的双价抗虫转基因大豆抗苜蓿夜蛾能力进行了分析。结果表明，与对照非转基因大豆相比，抗虫转基因大豆株系T5-150和 T5-195的叶片损害程度明显减轻；同时，苜蓿夜蛾幼虫食叶量显著下降、存活天数缩短，发育变慢，蛹化数也显著降低。表明抗虫转基因大豆抗苜蓿夜蛾能力显著提高。
Abstract:
Heliothis viriplaca is an important leaf-feeding insect for soybean.In this paper,resistance analysis of the stabilized binary insect-resistant transgenic soybean to H.viriplaca was conducted in vitro and in field conditions.The results showed that the insect-resistant transgenic lines T5-150 and T5-195 showed lower defoliation percentage than the non-transgenic ones.On the other hand,the H.viriplaca larvae feeding on the leaves of those two transgenic lines were characterized by less leaf consumption,greater larvae mortality,slower development,and less pupation ratio.Our results indicated that the resistance of the binary insect-resistant transgenic soybean to H.viriplaca was greatly enhanced.

参考文献/References:

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备注/Memo

基金项目：国家自然科学基金（C02020504）；吉林省科技发展计划项目（20050217-2，20060204）

作者简介：杨向东（1976-），男，助研，研究方向为大豆生物技术育种。Tel:0431-87063044；E-mail: xdyang020918@126.com
通讯作者：[HTSS]赵桂兰，研究员。Tel:0431-87063097；E-mail:zgl@cjaas.com

更新日期/Last Update: 2014-10-20