[1]李莉,孙欣,马君兰,等.异黄酮合成代谢调控关键酶CHS、CHI的特性与研究前景[J].大豆科学,2007,26(05):762-765.[doi:10.3969/j.issn.1000-9841.2007.05.026]
　LI Li,SUN Xin,MA Jun-lan,et al.PROGRESS ON KEY ENZYMES CHS、CHI OF ISOFLAVONES SYNTHESIZE[J].Soybean Science,2007,26(05):762-765.[doi:10.3969/j.issn.1000-9841.2007.05.026]
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异黄酮合成代谢调控关键酶CHS、CHI的特性与研究前景

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年05期 页码: 762-765 栏目: 出版日期: 2007-10-25
Title:
PROGRESS ON KEY ENZYMES CHS、CHI OF ISOFLAVONES SYNTHESIZE
文章编号:
1000-9841(2007)05-0762-04
作者:
李莉1; 孙欣1; 2; 马君兰1 ; 赵越1
1东北农业大学，哈尔滨 150030；
2黑龙江省农业科学院大豆研究所，哈尔滨150086
Author(s):
LI Li1; SUN Xin1; 2 ; MA Jun-lan1; ZHAO Yue1
1Northeast Agricultural University，Harbin 150030；
2Soybean Research Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086
关键词:
异黄酮; 查尔酮合酶（CHS）; 查尔酮异构酶（CHI）
Keywords:
Isoflavones; Chalcone synthase (CHS); Chalcone isomerase (CHI)
分类号:
Q814
DOI:
10.3969/j.issn.1000-9841.2007.05.026
文献标志码:
A
摘要:
查尔酮合酶(Chalcone synthase，CHS)与查尔酮异构酶(Chalcone isomerase，CHI)是异黄酮合成途径中的两个关键酶，它们在植物中的表达效率直接影响到异黄酮的产量。本文综述了植物CHS、CHI的功能、特性、基因结构、进化以及基因表达调控等方面研究的新进展，并对CHS、CHI研究的应用前景进行了展望，在此基础上提出了从根本上提高异黄酮产量的可行途径以及一些亟代解决的问题。
Abstract:
Chalcone synthase(CHS) and Chalcone isomerase(CHI) are the key enzymes in isoflavones synthesize，and variation of their expression might affect the content of isoflavones.The function，character，gene structure，gene evolution and expression control of CHS and CHI were summarized in this paper.The application foreground of CHS and CHI were also prospected，and the means to increase isoflavones were advised.

参考文献/References:

［1］谷利伟，谷文英，过世东.新型生长调节剂——异黄酮类植物雌激素[J].饲料添加剂，2000，21（12）：26－28.

[2]Shelagh R Muir，Geoff J Collins，Susan Robinson，et al.Over expression of petunia chalcone isomerase in tomato results in fruit containing increased levels of flavonols[J].Nature Biotechnology，2001，19：470-474.
[3]Karin Springob，Junichiro Nakajima，Mami Yamazaki，et al.Recent advances in the biosynthesis and accumulation of anthocyanins[J].Natural Product Report，2003，（20）：288-303.
[4]Kreuzaler F，Ragg H，Fautz E，et al.Uv-induction of chalcone synthase mRNA in cell suspension cultures of Pct-rose linum hotense[J].Proceedings National Academy of Science,USA，1983，80:2591-2593.
[5]Sommer H，Saedler H.Structure of the chalcone synthase gene of A ntirrhinum majus[J].Molecular and General Genetics，1986，202:429-434.
[6]王金玲，瞿礼嘉，陈军，等.CHS基因外显子2的进化规律及用于植物分子系统学研究的可行性[J].科学通报，2000,45（9）：942-950.
[7]Durbin M L，McCaig B，Clegg M T.Molecular evolution of the chalcone synthase multigene family in the morning glory genome [J].Plant Molecular Biology，2000,42:79-92.
[8]Schroder J，Raiber S，Berger T，et al.Plant polyketide synthase：a chalcone synthase-type enzyme which performs a condensation reaction with methylmalonyl-CoA in the biosynthesis of C-methylated chalcones[J].Biochemistry，1998，37：8417-8425.
[9]Arthur J M.Radiation and anthocyanin pigments in：Biological Effects of Radiation[M].Duggan B M ed.NewYork：McGraw Hill，1936:1109-1118.
[10]Thain S C，Murtas G，Lynn J R，et al.The circadian clock that controls gene expression in Arabidopsis is tissue specific[J].Plant Physiology，2002，130(1)：102-110.
[11]Leyva A，Jarillo J A，Salinas J，et al.Low temperature induces the accumulation of Phenylalanine Ammonia.Lyase and Chalcone Synthase mRNAs of Arabidopsis thaliana in a light-dependent manner[J].Plant Physiology，1995，108:39-46.
[12]Kaneko M，Itoh H，Inukai Y.Where do gibberellin biosynthesis and gibberellin signaling occur in rice plants[J].The Plant Journal，2003，35:104.
[13]Grotewold E，Peterson T.Isolation and characterization of a maize gene encoding chalcone flavonone isomerase[J].Molecular and General Genetics，1994，242 (1):1-8.
[14]Cho D S，Hong S H，Nam H G，Soh M S.FIN5 positively regulates far-red light responses in Arabidopsis thaliana[J].Plant Cell Physiology，2003，44(6)：565-572.
[15]Bieza K，Lois R.An Arabidopsis mutant tolerant to lethal ultraviolet.B levels shows constitutively elevated accumulation of flavonoids and other phenolics[J].Plant Physiology，2001，26(3)：1105.
[16]Jez J M，Noel J P.Reaction mechanism of chalcone isomerase pH dependence，diffusion control，and product binding differences[J].Journal of Biological Chemistry，2002，277(2):1361-1369.
[17]Norimoto Shimada，Toshio Aoki，Shusei Sato，et al.A cluster of genes encodes the two types of chalcone isomerase involved in the biosynthesis of general falavonoids and legume-specifics-deoxy（iso）Flavonoids in Lotus japonicas[J].Plant Physiology，2003，131（3）：941-951.
[18]Jez J M，Bowman M E，Dixon R A.Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase[J].Natural Structural Biology，2000，7(9):786-791.
[19]Gensheimer M，Mushegian A.Chalcone isomerase family and fold：no longer unique to plants[J].Protein Science，2004，13（2）：540-544.
[20]Tunen A J V，Koes R E，Mol J N M，et al.Cloning of the two chalcone flavanone isomerase genes from Petunia hybrid：coordinate，light-regulated and differential expression of flavonoid genes[J].EMBO J，1988，7：1257-1263.
[21]Tunen A J V，Hartman S A，Mol N M，et al.Regulation of chalcone isomerase （CHI）gene expression in petunia hydria：the use of alternative promoters in corolla，anthers and pollen[J].Plant Molecular Biology，1989，12：539-551.
[22]杨茂区，陈伟，冯磊，等.大豆异黄酮的生理功能研究进展[J].大豆科学，2006，3：320-324.
[23]Burbulis I E.Winkel-Shirley B.Interactions among enzymes of the Arabidopsis flavonoid biosynthetic pathway[J].Proceedings Natinonal Academy of Science，1999，96(22):29-34.

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备注/Memo

基金项目：黑龙江省教育厅资助课题（10551024）

作者简介：李莉(1977-)，女，硕士研究生，研究方向植物生理生化。
通讯作者：赵越，E-mail：yuezhao_2005@163.com; Tel：13936134146

更新日期/Last Update: 2014-10-20