LIU Ming,LUO Yuan-xu,NI Hui,et al.ACTIVE ANALYSIS OF SOYBEAN ANTIOXIDATIVE PEOTIDE FROM SOLID STATE FERMENTATION[J].Soybean Science,2007,26(03):381-385.[doi:10.3969/j.issn.1000-9841.2007.03.020]
固态发酵大豆制备的抗氧化肽的活性分析
- Title:
- ACTIVE ANALYSIS OF SOYBEAN ANTIOXIDATIVE PEOTIDE FROM SOLID STATE FERMENTATION
- 文章编号:
- 1000-9841(2007)03-0381-05
- 分类号:
- Q815S565.1
- 文献标志码:
- A
- 摘要:
- 综合评价用固态发酵法制备的大豆抗氧化肽的抗氧化活性。通过T-AOC方法测定总抗氧化能力、通过水杨酸法测定?OH、通过邻苯三酚法测定O-2、通过卵磷脂法测定脂质体过氧化、通过POV值法测定抗油脂自氧化。大豆抗氧化肽的总抗氧化能力为50.07U、对羟基自由基的清除率达到85.68%、抑制邻苯三酚自氧化能力达到23.61 U/mL、抑制脂质体过氧化率为34.58%、对油脂过氧化抑制抑制率为30%。用固态发酵法制备出的大豆抗氧化肽具有较强的还原能力、能清除羟基自由基、抑制邻苯三酚自氧化,并能够对脂质体过氧化和油脂过氧化具有一定的清除作用能力。
- Abstract:
- Synthesis analysed the antioxidative activity of soybean antioxidative peptide from solid state fermentation.Used the T-AOC method to measure the total antioxidative activity,used the salicylic acid method to measureOH ,and utilize the pyrogallic acid to measure O-2,made use of lecithin method to determine liposome oxidation and used the POV method to measure axunge oxidation .The total antioxidative activity as 50.07U,the ability of cleaning OH,O-2is 85.68% and 23.61 U/mL,the ability of restraining liposome oxidation and axunge oxidation is 34.58% and 28.52%.Soybean antioxidative peptide have the stronger deoxidize ability,it can clean away O-2andOH,and can also restrain liposome oxidation and axunge oxidation
参考文献/References:
[1]Harman D.Aging:a theory based on free radical and radiation chemistry[J]Gerontology1956,11:298-300.
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备注/Memo
基金项目:本项目获集美大学创新团队基金资助