[1]黄芸,崔力剑,窦玉红,等.紫外分光光度法测定大豆中异黄酮的含量[J].大豆科学,2007,26(02):273-275.[doi:10.3969/j.issn.1000-9841.2007.02.032]
　HUANG Yun,CU I Li-jian,DOU Yu-hong,et al.DETERMINATION OF SOYBEAN ISOFLAVONES WITH ULTRA-VIOLET SPECTROPHOTOMETRY[J].Soybean Science,2007,26(02):273-275.[doi:10.3969/j.issn.1000-9841.2007.02.032]
点击复制

紫外分光光度法测定大豆中异黄酮的含量

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年02期 页码: 273-275 栏目: 出版日期: 2007-04-25
Title:
DETERMINATION OF SOYBEAN ISOFLAVONES WITH ULTRA-VIOLET SPECTROPHOTOMETRY
文章编号:
1000-9841(2007)02-0273-03
作者:
黄芸; 崔力剑2; 窦玉红2; 王鑫国2; 牛丽颖2
1.河北医科大学生药学教研室，石家庄 050017；
2.河北医科大学中医学院，石家庄 050091
Author(s):
HUANG Yun1; CU I Li-jian2; DOU Yu-hong2; WANG Xin-guo2; NIU Li-ying2
1.Department of Pharmacognosy,Hebei Medical University,Shijiazhuang 050017；
2.Department of Traditional Chinese Medicine,Hebei Medical University,Shijiazhuang 050091
关键词:
大豆异黄酮; 氢氧化钠; 分光光度法; 含量测定
Keywords:
Soybean isoflavones; NaOH; UV spectrophtometry; Quantitative determination
分类号:
TS207.3
DOI:
10.3969/j.issn.1000-9841.2007.02.032
文献标志码:
A
摘要:
建立一种测定大豆中异黄酮类成分含量的紫外分光光度法分析方法。以染料木素为对照品，利用染料木素与氢氧化钠产生反应，在271 nm波长处有最大吸收峰，用紫外分光光度法测定大豆中总异黄酮的含量。大豆中总异黄酮的含量为4.784 mg/g，加样回收率为100.9%，相对标准偏差为2.0%。方法简便、准确、重现性好，可作为检查大豆中异黄酮的含量的一种手段，适用于大豆及其保健品的日常分析和质量控制。
Abstract:
A UV spectrophotometry used for determination of soybean isoflavones was established.Genistein was selected as the reference substance.Based on the reaction of genistein and NaOH,which has the maximum absorption in 271 nm wavelength,ultra-violet spectrophotometry method was used for determination of isoflavones in Glycine max(L.) Merr..The total content of isoflavones in soybean extracts was 4.784 mg/g with mean recovery 100.9%,and the RSD was 2.0%.The method is suitable for the routine analysis and quality control of the soybean and function food with easy-to-handle,good accuracy and reproducibility.

参考文献/References:

［1］牛丽颖,王鑫国,葛喜珍,等.淡豆豉提取物降糖有效部位研究[J].中药药理与临床,2002,20(5):21-22.

［2］C．Lee Holder，Mona I.Churchwell，Daniel R.Doerge.Quantification of Soy Isoflavones，Genistein and Conjugates in Rat Blood Using LC/ES-MS［J］.Agriculture Food Chemistry.，1999，47：3764-3770.
［3］张立.RP-HPLC法测定大豆提取物中大豆苷元、染料木素、大豆苷、染料木苷的含量[J].中草药,2001,32(2):118-120.
［4］G S Mcleod ,M J Sheperd.Determination of the ionization constants of isoflavonrs by capillary electrophoresis [J].Czech Journd of Food Science,1999,17(2):61-67.
［5］张玉梅,孙学斌,高旭年,等.紫外分光光度法测定大豆总异黄酮的含量[J].中国食品卫生杂志,2000,12(4):7-8.
［6］崔力剑,黄芸,杜淑娟,等.紫外分光光度法测定淡豆豉中异黄酮的含量[J].河北中医药学报,2004,19(3):32-33.
［7］袁金斌,卢建中.紫外分光光度法测定大豆总异黄酮的含量[J].大豆科学,2004,23(2):147-150.
［8］窦玉红,崔力剑,黄芸,等.正交设计优选淡豆豉中总异黄酮的提取工艺[J].河北中医药学报,2006,21(2):33-35.

相似文献/References:

[1]徐美蓉,李玉芳,董煚,等.大豆异黄酮超声波辅助提取条件优化的研究[J].大豆科学,2013,32(01):102.[doi:10.3969/j.issn.1000-9841.2013.01.024]
　XU Mei-rong,LI Yu-fang,DONG Jiong,et al.Optimization of Soy Isoflavone Extraction Assisted with Ultrasonic[J].Soybean Science,2013,32(02):102.[doi:10.3969/j.issn.1000-9841.2013.01.024]
[2]陈玉胜.大豆异黄酮抗高尿酸血症活性研究初探[J].大豆科学,2013,32(02):279.[doi:10.3969/j.issn.1000-9841.2013.02.032]
　CHEN Yu-sheng.Study on Anti-hyperuricemia Activity of Soybean Isoflavone[J].Soybean Science,2013,32(02):279.[doi:10.3969/j.issn.1000-9841.2013.02.032]
[3]沈丹萍,王小平,秦立强.大豆异黄酮摄入与卵巢癌关系的Meta分析[J].大豆科学,2013,32(06):814.[doi:10.11861/j.issn.1000-9841.2013.06.0814]
　SHEN Dan-ping,WANG Xiao-ping,QIN Li-qiang.Isoflavones Intake and Risk of Ovarian Cancer:A Meta Analysis of Epidemiological Study[J].Soybean Science,2013,32(02):814.[doi:10.11861/j.issn.1000-9841.2013.06.0814]
[4]何恩铭,沈瑞池,王 伟,等.紫外分光光度法测定豆渣提取物中大豆异黄酮含量的研究[J].大豆科学,2013,32(06):818.[doi:10.11861/j.issn.1000-9841.2013.06.0818]
　HE En-ming,SHEN Rui-chi,WANG Wei,et al.Determination of Isoflavones in Extraction of Soybean Dregs Using Ultraviolet Spectrophotometry[J].Soybean Science,2013,32(02):818.[doi:10.11861/j.issn.1000-9841.2013.06.0818]
[5]彭 游,喻国贞,郎少杰,等.新型大豆异黄酮萘磺酸酯前药的设计合成[J].大豆科学,2013,32(06):854.[doi:10.11861/j.issn.1000-9841.2013.06.0854]
　PENG You,YU Guo-zhen,LANG Shao-jie,et al.Design and Synthesis of Novel Genistein Naphthylsulfonate Prodrugs[J].Soybean Science,2013,32(02):854.[doi:10.11861/j.issn.1000-9841.2013.06.0854]
[6]刘少静,王多宁,刁颖博,等.大豆异黄酮纯化工艺研究[J].大豆科学,2013,32(04):535.[doi:10.11861/j.issn.1000-9841.2013.04.0535]
　LIU Shao-jing,WANG Duo-ning,DIAO Ying-bo,et al.Optimization on the Purification Technologies of Soybean Isoflavone[J].Soybean Science,2013,32(02):535.[doi:10.11861/j.issn.1000-9841.2013.04.0535]
[7]尹学哲,金延华,金梅花,等.大豆异黄酮对内毒素及D-氨基半乳糖所致小鼠急性肝损伤的保护作用[J].大豆科学,2013,32(04):544.[doi:10.11861/j.issn.1000-9841.2013.04.0544]
　YIN Xue-zhe,JIN Yan-hua,JIN Mei-hua,et al.Protective Effects of Soy Isoflavones on Acute Liver Injury Induced by Lipopolysaccharide and D-galactosamine in Mice[J].Soybean Science,2013,32(02):544.[doi:10.11861/j.issn.1000-9841.2013.04.0544]
[8]董军奎,葛晓月.大豆异黄酮：一种多功能植物药[J].大豆科学,2013,32(04):565.[doi:10.11861/j.issn.1000-9841.2013.04.0565]
　DONG Jun-kui,GE Xiao-yue.Soy Isoflavone:The Multipurpose Phytopharm[J].Soybean Science,2013,32(02):565.[doi:10.11861/j.issn.1000-9841.2013.04.0565]
[9]李怡然,赵丽芹,李爱科,等.豆浆中水溶性大豆异黄酮检测方法的建立[J].大豆科学,2014,33(03):429.[doi:10.11861/j.issn.1000-9841.2014.03.0429]
　LI Yi-ran,ZHAO Li-qin,LI Ai-ke,et al.Construction of Detection Method for Water soluble Isoflavone Content in Soybean Milk[J].Soybean Science,2014,33(02):429.[doi:10.11861/j.issn.1000-9841.2014.03.0429]
[10]刘中华,胡春红,郭 婕,等.低温豆粕中异黄酮和皂甙的微波同步提取工艺[J].大豆科学,2014,33(03):443.[doi:10.11861/j.issn.1000-9841.2014.03.0443]
　LIU Zhong-hua,HU Chun-hong,GUO Jie,et al.Microwave Extraction of Isoflavones and Saponins in the Low temperature ?Soybean Meal[J].Soybean Science,2014,33(02):443.[doi:10.11861/j.issn.1000-9841.2014.03.0443]

备注/Memo

基金项目：河北省科学技术研究与发展计划资助项目（05276101D-78）

作者简介：黄芸（1974 -），女，博士，讲师，主要从事天然产物活性成分研究与开发。 Tel: 0311-86265628

更新日期/Last Update: 2014-10-22