Yan Shengrong Zhang Lan Zhou Qing.EFFECTOF LANTHANUM ON MEMBRANE LIPID PREOXIDATION OFSOYBEAN SEEDLINGS UNDES SUPPLEMENTAL UV- BIRRADIATION[J].Soybean Science,2006,25(02):145-148.[doi:10.11861/j.issn.1000-9841.2006.02.0145]
镧对 UV - B 胁迫下大豆幼苗膜脂过氧化影响*
- Title:
- EFFECTOF LANTHANUM ON MEMBRANE LIPID PREOXIDATION OFSOYBEAN SEEDLINGS UNDES SUPPLEMENTAL UV- BIRRADIATION
- 文献标志码:
- A
- 摘要:
- 采用水培实验方法进行 LaCl 3 对大豆(Clycine max )幼苗最适喷施浓度的筛选, 并在此基础上初步研究了 La 对紫外辐射(UV - B:0. 15W cm- 2, 0. 30W cm- 2 )胁迫下大豆幼苗膜脂过氧化的影响. 实验结果表明, 20mg L- 1La 能显著提高大豆叶片 CAT 、POD 等抗氧化酶活性, 降低细胞质膜透性和 MDA 含量 ,改善大豆幼苗抗氧化代谢功能. La +UV - B 和 UV - B 组细胞质膜透性和 MDA 含量的动态走势相近 ,仅为前者的增幅低于后者, La +T 1 组的增幅低于 La +T 2 ,表明 La 能够缓解 UV - B 辐射造成的膜脂过氧化作用 ,且 La 对 T 1 的防护效果优于 T 2 。
- Abstract:
- Effect of lanthanum (La 3 + )on membrane lipid peroxidation of soybean seedlings under elevatedultraviolet - B radiation (UV - B :280~ 330nm)at 15μW /cm 2 and 30μW /cm 2 level was studied with hydro-ponics under laboratory conditions. Firstly , such indexes of plasmolemma permeality , MDA content , CATand POD activities were utilized to choose the best spraying concentration of rare earth to soybean seed-lings. Our result showed that 20mg L - 1La significantly increased activities of CAT and POD , decreasedplasmolemma permeality and MDA content and that improved antioxidant metabolism of soybean seed-lings. The plasmolemma permeality and MDA content of soybean seedlings gradually increased during sup-plemental ultraviolet - B radiation and then decreased after ultraviolet - B stress. The dynamic trend ofplasmolemma permeality and MDA content in soybean seedlings exposed to ultraviolet - B radiation withthe pretreatment of lanthanum was similar to the above UV - B treatment. However , the range of varia-tion of UV - B and La treatment was lower than only UV - B treatment ;that of low dosage of UV - B andLa treatment was also lower than high dosage of UV - B and La treatment. It could be concluded that Lacould mitigate membrane lipid peroxidation caused by UV - B stress and that its protective effects againstlow UV - B radiation was better than that of high UV - B radiation.
参考文献/References:
1 UNEP , 2002. Executive Summary. Final of UNEP /WMO Scien-tific Assessment of Ozone Depletion :2002. Prepared by the Sci-entific A ssessment Panel of the Montreal Protocol on Substancesthat Deplete the Ozone Layer[ R] . UNEP, Nairobi(released 23August 2002)
备注/Memo
基金项目:国家发改委稀土专项基金(GFZ040628)与江苏省科技厅高新技术项目基金(GB2005040)资助作者简介:闫生荣(1981- ), 男, 硕士研究生。联系人:周青, E - mail:zhouqeco @y ahoo. com. cn.