[1]王 　惠　段玉玺 *　陈立杰　邱丽娟　常汝镇.一个与大豆黄色种皮相关的 RAPD 标记*[J].大豆科学,2005,24(01):17-20.[doi:10.11861/j.issn.1000-9841.2005.01.0017]
　Wang Hui Duan Yuxi * Chen Lijie Qiu Lijuan Chang Ruzhen.A RAPD MARKER RELATED WITH YELLOW SEED COATOF SOYBEAN[J].Soybean Science,2005,24(01):17-20.[doi:10.11861/j.issn.1000-9841.2005.01.0017]
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一个与大豆黄色种皮相关的 RAPD 标记*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第24卷 期数: 2005年01期 页码: 17-20 栏目: 出版日期: 2005-02-25
Title:
A RAPD MARKER RELATED WITH YELLOW SEED COATOF SOYBEAN
作者:
王 　惠1　段玉玺1 *　陈立杰1　邱丽娟2　常汝镇2
(1. 沈阳农业大学 植物线虫学研究室 ,沈阳 110161 ;2. 中国农科院作物所农业部作物种质资源与生物技术重点开放实验室, 北京 100081)
Author(s):
Wang Hui 1 　Duan Yuxi 1 * 　Chen Lijie 1 　Qiu Lijuan 2 　Chang Ruzhen 2
(1. Nematology Lab, Shenyang Agricultural University , Shenyang 110161 ;2. Key Lab of CropGermplasm &Biotechnology , Ministry of Agriculture , Instituteof Crops, Chinese Academy ofAgriculture Science , Beijing 100081)
关键词:
RAPD ; 分子标记 ; 大种皮颜色
Keywords:
RAPD; Molecular marker ; Seed coat color
DOI:
10.11861/j.issn.1000-9841.2005.01.0017
文献标志码:
A
摘要:
利用BSA 法对11个黑色种皮的大豆品种和 12 个黄色种皮的大豆材料的基因组 DNA 进行了 RAPD 分析, 供试的 100 个随机引物有 66 个产生了 RAPD 扩增产物 ,其中产生 RAPD 多态性的随机引物有 15 个 。在所产生的扩增产物中 , 获得了一个与大豆种皮色相关的特异 DNA 片段S79500 。同时,采用该标记对辽豆 10 号×PI437654 杂交后代种皮颜色有分化的群体和黄 、青 、黑、褐色种皮的大豆进行检测 ,经验证这个 RAPD 标记具有较高的重复性和稳定性 ,可用于辅助选育优良的黄色种皮的大豆新品种 。
Abstract:
BSA method was employed to analyze genome DNA of 11 black coat seed soybean and 12 yellowcoat seed soybean by RAPD method. The bands were produced for 66 primers among 100 primers. Therewere polymorphism bands for 15 primers. In all products amplified, a special DNA fragment , S79500 wasclosely related with the yellow coat of soybeans. The primer S79500 became a RAPD marker for the yellowcoat of soybean. In another hand, the S79500 also was used to test the filial generation with yellow ,green, black and brown coat from Liaodou 10 ×PI437654. The results showed that the S79500 was verystable and repeatable. The RAPD marker S79500 was useful to assistant the selection for soybean hybridbreeding.

参考文献/References:

1　Michelmore R W , Paran I, Kesseli R V. Identification of markerslinked to disease - resistance genes by using segregating analysis:A rapid method to detect markers in specific genomic regions byusing segregating populations[ J] . Proc Natl Acad Science USA ,1991, 88:9828 - 9832.

2　关荣霞, 常汝镇, 邱丽娟, 等. 用于 SSR 分析的大豆 DNA 的快速提取[ J] . 大豆科学, 2003, 22(1):73 - 74.
3 　Matson. A. L. Evidence of a fourth gene for resistance to soybeancyst nematode[ J] . Crop Science, 1965, 5:477.
4　Williams J G K , Kubelic A R. DNA polymorphism amplified byarbitrary primers are useful genetic marker[ J] . Nucleic AcidsRes, 1990, 18(22):6531 - 6535.
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备注/Memo

基金项目:国家自然科学基金课题(39770494)资助

作者简介:王惠(1972 -), 女, 博士研究生, 主要从事抗大豆胞囊线虫基因的抗性机制和分子标记研究。 E- mail:wanghuisy nd@yahoo.com. cn
通讯作者:段玉玺*(1964 -), 教授, 博士生导师, Tel:024- 88454528, Fax :024 - 88492630, E - mail:duanyx @syau. edu. cn

更新日期/Last Update: 2016-12-22