[1]王萍　王罡 　季静　周智明　郭威　拉巴　吴颖.农杆菌介导大豆未成熟子叶的遗传转化[J].大豆科学,2004,23(02):86-90.[doi:10.11861/j.issn.1000-9841.2004.02.0086]
　Wang Ping Wang Gang Ji Jing Zhou Zhiming Gui Wei La Ba Wu Ying.GENETIC TRANSFORMATION OF IMMATURE COTYLEDON VIA AGROBACTERIUMTUMEFACIENS IN SOYBEAN[J].Soybean Science,2004,23(02):86-90.[doi:10.11861/j.issn.1000-9841.2004.02.0086]
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农杆菌介导大豆未成熟子叶的遗传转化

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第23卷 期数: 2004年02期 页码: 86-90 栏目: 出版日期: 2004-05-25
Title:
GENETIC TRANSFORMATION OF IMMATURE COTYLEDON VIA AGROBACTERIUMTUMEFACIENS IN SOYBEAN
文章编号:
1000 -9841(2004)02 -0086 -05
作者:
王萍　王罡 　季静　周智明　郭威　拉巴　吴颖
解放军军需大学植物基因工程研究中心, 长春130062
Author(s):
Wang Ping 　Wang Gang 　Ji Jing 　Zhou Zhiming 　Gui Wei 　La Ba 　Wu Ying
Research Center For Plant Genet ic Engineering , Changchun Universi ty of Agricultural andAnimal Sciences , Changchun 130062
关键词:
大豆(Glycine max ); 农杆菌; 遗传转化; PCR 检测
Keywords:
Soybean (Glycine max); Agrobacterium tumefaciens ; Genetic t ransfo rmation ; PCR detection
分类号:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2004.02.0086
文献标志码:
A
摘要:
用农杆菌LBA4404(pGBI121S4ABC)转化5 个大豆品种的未成熟子叶, 经卡那霉素筛选得到抗性植株, 进一步用PCR 检测, 获得10 株PCR 阳性植株。同时发现, 不同大豆品种农杆菌转化的效率是不同的, 吉林43 较易于转化;共培养时间是影响转化效率的主要因子。
Abstract:
Immature cotyledon of 5 dif ferent soybean varieties were t ransfo rmed by Ag robacterium tumefaciensLBA4404(pGBI121S4ABC)and resistant plants to kanamy cin w ere obtained .10 egenerated plant s g ave positivePCR reaction .Transfo rmation ef ficiency w as different among 5 varieties .Jilin 43 was easy for t ransforming byAgrobacterium tumefaciens .The time of co -cultivation is one of the most impo rtant factors for transformat ioneff iciency .

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备注/Memo

基金项目:国家植物转基因中试及产业化基地专项基金(项目编号J99 -B -001)

作者简介:王萍(1957 -), 女, 教授, 博士, 主要从事作物遗传育种与植物转基因研究。E -mail:y_pw ang @yahoo .com .cn

更新日期/Last Update: 2016-12-31