[1]杨宏顺陈复生.反胶束中蛋白酶对前萃取蛋白和油脂的影响[J].大豆科学,2003,22(04):257-260.[doi:10.11861/j.issn.1000-9841.2003.04.0257]
　Yang Hongshun Chen Fusheng.THE EFFECT OF PROTEASES IN REVERSE MICELLES ON THE FORWARDEXTRACTION OF SOYBEAN PROTEIN AND OIL[J].Soybean Science,2003,22(04):257-260.[doi:10.11861/j.issn.1000-9841.2003.04.0257]
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反胶束中蛋白酶对前萃取蛋白和油脂的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第22卷 期数: 2003年04期 页码: 257-260 栏目: 出版日期: 2003-11-25
Title:
THE EFFECT OF PROTEASES IN REVERSE MICELLES ON THE FORWARDEXTRACTION OF SOYBEAN PROTEIN AND OIL
文章编号:
1000 -9841(2003)04 -0257 -04
作者:
杨宏顺1陈复生2
1 .上海交通大学机械与动力工程学院, 上海200030 ;
2 .郑州工程学院粮油食品学院, 郑州450052
Author(s):
Yang Hongshun1 　Chen Fusheng2
1.School of Mechanical and Dynamic Engineering , Shanghai J iaotong University , Shanghai , 200030 ;
2 .School of Grain &Oil Food , Zhengzhou Institute of Technology , Zhengzhou , 450052
关键词:
反胶束; 大豆蛋白; 油脂; 蛋白酶; 前萃
Keywords:
Reverse micelles ; Soybean protein ; Oil ;  protease ; Forward extraction
分类号:
TS 201.2
DOI:
10.11861/j.issn.1000-9841.2003.04.0257
文献标志码:
A
摘要:
在AOT/异辛烷反胶束体系中加入蛋白酶, 研究蛋白酶对豆粉萃取分离大豆蛋白和油脂的影响。在40 -50 ℃, 对豆粉进行80 ℃, 2min 水浴预处理可提高蛋白的萃取率。与不加酶相比, 在35 -40 ℃蛋白酶可以显著提高反胶束中大豆蛋白的萃取率, 在40 ℃时, 以0 .2mol·L -1 的pH 值为8 .0的枯草杆菌中性蛋白酶AS1 .398 液作增溶液的反胶束萃取, 蛋白前萃取率达到65 .5 %, 不加酶时为56 .8 % .萃取120min时, 添加中性蛋白酶AS1 .398和碱性蛋白酶2709组的油脂的前萃取率比不加蛋白酶组低1 .75 %和0 .877 %.萃取60min时反胶束的油脂的萃取率为正己烷萃取的94 .68 %.表明蛋白酶对蛋白质进入水核过程或进入后可能起作用, 对萃取油脂作用不显著。
Abstract:
The proteases were added into the AOT/iso -octane reverse micelles to study the effects of the proteases onthe extractions of soybean protein and oil from soybean powder .Between 40 ℃ and 50 ℃, pretreatment of the soybeanpowder with 80 ℃, 2min water bath can increased the protein extraction rate .Contrast with no proteases added , proteasescould increase the protein extraction rate at 35 -40 ℃.At 40 ℃, the forward protein extraction ratewas 65 .5 %when 0 .2mol L-1 pH8 .0 neutrase AS1 .398 solution dissolved ;56 .8 %with no proteases but only KCl solution in reverse micelles.At 120min , the oil extraction rate of reverse micelles with AS1 .398 and Alkalase 2709 were 1 .75 % and 0 .877 % respectively lower than that with no proteases .At 60min , the oil extraction rate of reverse micelles was 94 .68 %of that with hexane extraction .It showed proteases have some actions on the protein before or after itdissolved into the waternucleus of reverse micelles , nowever ,with no signifluence on extraction .

参考文献/References:

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2 　杨宏顺, 陈复生, 于志玲, 等.反胶束萃取技术在食品科学中的研究进展[ J] .郑州工程学院学报,2001 , 22(4):66 -70 .
3 　杨宏顺, 陈复生, 赵俊廷.反胶束溶液同时分离大豆蛋白和油脂的前萃研究[ J] .食品科技, 2002 , 1 :16 -18 .
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6 　杨宏顺, 陈复生, 李云飞, 等.反胶束中碱性蛋白酶2709 活力的研究[ J] .食品科学, 2002 , 24(4):1-4 .
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备注/Memo

基金项目:河南省科技攻关项目(971070103)　

作者简介:杨宏顺(1977-), 男, 博士生, 研究方向食品传热质工程。

更新日期/Last Update: 2016-12-31