[1]陈宏宇　文景芝　于鑫.大豆疫霉菌与其它几种疫霉菌同工酶电泳比较研究[J].大豆科学,2001,20(01):1-4.[doi:10.11861/j.issn.1000-9841.2001.01.001]
　Chen Hong yuWen Jing zhiYu Xin.STUDIES ON ISOZYME ELECTROPHORESIS OF Phytophthora sojaeAND OTHER Ph ytophthora SPECIES[J].Soybean Science,2001,20(01):1-4.[doi:10.11861/j.issn.1000-9841.2001.01.001]
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大豆疫霉菌与其它几种疫霉菌同工酶电泳比较研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第20卷 期数: 2001年01期 页码: 1-4 栏目: 出版日期: 2001-02-25
Title:
STUDIES ON ISOZYME ELECTROPHORESIS OF Phytophthora sojaeAND OTHER Ph ytophthora SPECIES
文章编号:
1000- 9841( 2001) 01- 0001- 04
作者:
陈宏宇　文景芝　于鑫
东北农业大学植物保护系, 150030
Author(s):
Chen Hong yu　Wen Jing zhi　Yu Xin
Department of Plant Protection , Northeast Agriculture University , Harbin, 150030
关键词:
大豆疫霉菌;  酯酶;  超氧化物歧化酶; 淀粉酶;  过氧化氢酶;  同工酶
Keywords:
Phytophthora sojae ;  Esterase;  Seproxide di smutase;  Diastase;  Catalase;  Iso zyme
分类号:
S435. 651. 1+ 6
DOI:
10.11861/j.issn.1000-9841.2001.01.001
文献标志码:
A
摘要:
本文对大豆疫霉菌( Phy tophthora sojae )的15个菌株及其它7种疫霉的8个菌株进行了4种同工酶电泳比较研究。结果表明,大豆疫霉菌种内菌株间4种同工酶基本一致。疫霉属种间酯酶( EST)和超氧化物歧化酶( SOD)酶谱存在明显差异,表现为酶带的多少和Rf 值的不同; 淀粉酶( DIA)和过氧化氢酶( CAT)同工酶酶谱差异不很明显,与某些疫霉种酶谱相似,只表现为酶带强度上的差异。本文首次报道了大豆疫霉菌的SO D、CAT和DIA同工酶,并指出EST和SOD同工酶在P . sojae种的鉴定上具有重要意义,为P. sojae种的鉴定提供一个辅助性手段。
Abstract:
Polyacrylamicide g el elect ropho retic analysis o f i so zymes of 15 st rains o f P. sojae and 8 st rainso f sev en o ther Ph ytophthora species w as studied. The result s show higher similari ties betw een 15 st rainsf rom P. sojae in four iso zyme zymog rams. There a re no ma rked di fferences o f DIA a nd CAT zymog ramsbetw een P. sojae and sev en o ther Ph ytophthora species. Marked dif ferences o f EST and SOD zymog rams among P. so jae a nd sev en o ther Ph ytophthora species indica te that ES T and SOD i so zyme are useful and reliablesupplementary tool s fo r identificatio n of P. sojae.

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备注/Memo

项目来源: 省科委重点攻关项目

作者简介: 陈宏宇( 1976- ) ,女,东北农业大学植物病理专业在读研究生。

更新日期/Last Update: 2016-12-28