[1]刘丽君吴俊江高杰汪清胤黄永芬夏凯李文华.转ipt基因大豆感染SMV 1号的生理特性变化[J].大豆科学,1999,18(03):213-218.[doi:10.11861/j.issn.1000-9841.1999.03.0213]
　Liu Lijun Wu Junjiang Gao Mingjie WangQingyin HuangYongfen Xia Kai Li Wenhua.THE PHYSIOLOGY CHANGES OF SOYBEANS AFIER TRANSFER OF ipt GENE INTO SOYBEAN BY Ti PLASMID AFTER INOCULAING WITH SMV NO.1[J].Soybean Science,1999,18(03):213-218.[doi:10.11861/j.issn.1000-9841.1999.03.0213]
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转ipt基因大豆感染SMV 1号的生理特性变化

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第18卷 期数: 1999年03期 页码: 213-218 栏目: 出版日期: 1999-08-25
Title:
THE PHYSIOLOGY CHANGES OF SOYBEANS AFIER TRANSFER OF ipt GENE INTO SOYBEAN BY Ti PLASMID AFTER INOCULAING WITH SMV NO.1
作者:
刘丽君1吴俊江1高杰1汪清胤2黄永芬2夏凯3李文华4
1黑龙江省农科院大豆研究所　150086?
2哈尔滨师范大学生物系
3南京农业大学　
4东北农业大学农学院
Author(s):
Liu Lijun1 Wu Junjiang1 Gao Mingjie1 WangQingyin2 HuangYongfen2 Xia Kai3 Li Wenhua4
1 The Soybean Reseatch Institute,Heilongjiang Academy of Agricultwral Science
2 Department of Biology Harbin Normal University?
3 Naning Agricultural Universisy
4 Northeast Agricultural University
关键词:
ipt基因;  转基因植株;  病毒病
Keywords:
ipt gene;  Tramsformed plant;  SMV
DOI:
10.11861/j.issn.1000-9841.1999.03.0213
文献标志码:
A
摘要:
本文对黑龙江省推广的三个大豆品种及它的转ipt基因株系F4 ,采用生化技术酶联免疫检测技术( ELISA) ,研究了病毒病侵染条件下,转基因植株的生化特性变化及对内源激素含量及其平衡状况的影响。实验结果表明: SMV 1号诱导条件下,转基因大豆细胞中MDA含量减少, SOD酶活性较强;转7SL- ipt基因的大豆,内源激素IPA、IAA、GA含量明显增加;而转8SL- ipt基因的大豆,则通过内源激素IAA、ABA含量的增加,来改变大豆的形态发生和提高抗病性。
Abstract:
Three cultivars of soybean of Heilongjiang Province have been transformed with iptgene into the soybean plants.The ELISA was used to study the endogenous hormonal amount and their balance condition and physiological change after the ipt gene being tramsformed into soybean plants.The results showed that the content of MDA became lower and the activity of SOD enzyme became higher after the tramsformation ipt into soybean plant cell by inoculation with SMV NO.1.The amount of endogenous IPA、IAA、GA,were higher in the soybean plant transformed with 8SL-pit and caused mophologicol change of the plant and increasing resistante to diseases of soybean.

参考文献/References:

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更新日期/Last Update: 2016-12-27